scholarly journals GUAIFENESIN MODIFIES AIRWAY MUCUS, REDUCES MUCUS-CELL SURFACE INTERACTIONS, AND INCREASES MUCOCILIARY TRANSPORT IN AN ORGANOTYPIC IN VITRO MODEL OF MUCUS HYPERSECRETION

CHEST Journal ◽  
2020 ◽  
Vol 158 (4) ◽  
pp. A1682-A1683
Author(s):  
Phillip Clapp ◽  
Holly Thacker ◽  
Ly Nguyen ◽  
Miriam Figueira ◽  
Karin Nicholson ◽  
...  
Keyword(s):  
Cell Surface ◽  
In Vitro Model ◽  
Surface Interactions ◽  
Mucociliary Transport ◽  
Mucus Cell ◽  
Mucus Hypersecretion ◽  
Cell Surface Interactions ◽  
Airway Mucus ◽  
Vitro Model

scholarly journals Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

Materials ◽  
2021 ◽  
Vol 14 (19) ◽  
pp. 5858
Author(s):  
Ana Carolina Batista Brochado ◽  
Victor Hugo de Souza ◽  
Joice Correa ◽  
Suzana Azevedo dos Anjos ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
...  
Keyword(s):  
Cell Surface ◽  
3D Model ◽  
Bone Cells ◽  
Three Dimensional ◽  
Surface Interactions ◽  
Force Microscopy ◽  
Tissue Therapy ◽  
Cell Surface Interactions ◽  
Titanium Surfaces

Successful biomaterials for bone tissue therapy must present different biocompatible properties, such as the ability to stimulate the migration and proliferation of osteogenic cells on the implantable surface, to increase attachment and avoid the risks of implant movement after surgery. The present work investigates the applicability of a three-dimensional (3D) model of bone cells (osteospheres) in the evaluation of osteoconductive properties of different implant surfaces. Three different titanium surface treatments were tested: machined (MA), sandblasting and acid etching (BE), and Hydroxyapatite coating by plasma spray (PSHA). The surfaces were characterized by Scanning Electron Microscopy (SEM) and atomic force microscopy (AFM), confirming that they present very distinct roughness. After seeding the osteospheres, cell–surface interactions were studied in relation to cell proliferation, migration, and spreading. The results show that BE surfaces present higher densities of cells, leaving the aggregates towards than titanium surfaces, providing more evidence of migration. The PSHA surface presented the lowest performance in all analyses. The results indicate that the 3D model allows the focal analysis of an in vitro cell/surfaces interaction of cells and surfaces. Moreover, by demonstrating the agreement with the clinical data observed in the literature, they suggest a potential use as a predictive preclinical tool for investigating osteoconductive properties of novel biomaterials for bone therapy.

scholarly journals Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

2007 ◽  
Vol 2007 ◽  
pp. 1-19 ◽  
Author(s):  
M. Jäger ◽  
C. Zilkens ◽  
K. Zanger ◽  
R. Krauspe
Keyword(s):  
Cell Surface ◽  
Cell Types ◽  
Surface Interactions ◽  
Orthopaedic Implants ◽  
Nanostructured Surfaces ◽  
Major Interest ◽  
Cell Surface Interactions ◽  
Biomaterial Application ◽  
Different Cell Types

Cell-surface interactions play a crucial role for biomaterial application in orthopaedics. It is evident that not only the chemical composition of solid substances influence cellular adherence, migration, proliferation and differentiation but also the surface topography of a biomaterial. The progressive application of nanostructured surfaces in medicine has gained increasing interest to improve the cytocompatibility and osteointegration of orthopaedic implants. Therefore, the understanding of cell-surface interactions is of major interest for these substances. In this review, we elucidate the principle mechanisms of nano- and microscale cell-surface interactions in vitro for different cell types onto typical orthopaedic biomaterials such as titanium (Ti), cobalt-chrome-molybdenum (CoCrMo) alloys, stainless steel (SS), as well as synthetic polymers (UHMWPE, XLPE, PEEK, PLLA). In addition, effects of nano- and microscaled particles and their significance in orthopaedics were reviewed. The significance for the cytocompatibility of nanobiomaterials is discussed critically.

scholarly journals Desmosome development in an in vitro model.

1980 ◽  
Vol 85 (3) ◽  
pp. 695-702 ◽  
Author(s):  
H M Dembitzer ◽  
F Herz ◽  
A Schermer ◽  
R C Wolley ◽  
L G Koss
Keyword(s):  
Cell Surface ◽  
In Vitro Model ◽  
Human Cancer ◽  
Filament Formation ◽  
Extracellular Material ◽  
Close Apposition ◽  
Mixed Suspension ◽  
Cancer Line ◽  
Vitro Model

A model has been devised to study the in vitro formation of desmonsomes. The model is based on the differential labeling of two subpopulations of a desmosome-forming human cancer line (C4I). The labeled subpopulations are dispersed, preincubated separately on a shaking water bath for 24 h to allow the internalization of desmosome fragments and the repair of the cell surface, and then mixed, and allowed to aggregate. Aliquots of the mixed suspension are fixed at various intervals. The time between mixing and fixation represents the maximum age of any junction between dissimilarly labeled cells. The beginnings of desmosome formation were observed within a few minutes after the beginning of aggregation. Close apposition of cell membranes was seen immediately after mixing, followed within 15 min by the appearance of a submembrane density in one or both of the interacting cells. Intracytoplasmic filament formation takes place at between 15 and 30 min. Desmosome formation is complete by 90 min. The process is accompanied by a progressive widening of the extracellular space and the desification and organization of the extracellular material and the submembrane plaques.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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