Combinatorial Models of Gene Assembly

2005 ◽  
pp. 188-195
Author(s):  
Tero Harju
Keyword(s):  
Gene Assembly

scholarly journals Functional cysteine-less subunits of the transporter associated with antigen processing (TAP1 and TAP2) by de novo gene assembly

FEBS Letters ◽  
2002 ◽  
Vol 533 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Susanne Heintke ◽  
Min Chen ◽  
Ulrike Ritz ◽  
Brigitte Lankat-Buttgereit ◽  
Joachim Koch ◽  
...  
Keyword(s):  
Antigen Processing ◽  
De Novo ◽  
Gene Assembly ◽  
De Novo Gene

Parallelism in Gene Assembly

2005 ◽  
pp. 138-148 ◽  
Author(s):  
Tero Harju ◽  
Chang Li ◽  
Ion Petre ◽  
Grzegorz Rozenberg
Keyword(s):  
Gene Assembly

scholarly journals Hierarchical assembly and disassembly of a transcriptionally active RAG locus in CD4+CD8+ thymocytes

2018 ◽  
Vol 216 (1) ◽  
pp. 231-243 ◽  
Author(s):  
Abani Kanta Naik ◽  
Aaron T. Byrd ◽  
Aaron C.K. Lucander ◽  
Michael S. Krangel
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
Molecular Mechanisms ◽  
Promoter Activation ◽  
Active Chromatin ◽  
Gene Assembly ◽  
Down Regulation ◽  
Hierarchical Assembly ◽  
Rag2 Gene

Expression of Rag1 and Rag2 is tightly regulated in developing T cells to mediate TCR gene assembly. Here we have investigated the molecular mechanisms governing the assembly and disassembly of a transcriptionally active RAG locus chromatin hub in CD4+CD8+ thymocytes. Rag1 and Rag2 gene expression in CD4+CD8+ thymocytes depends on Rag1 and Rag2 promoter activation by a distant antisilencer element (ASE). We identify GATA3 and E2A as critical regulators of the ASE, and Runx1 and E2A as critical regulators of the Rag1 promoter. We reveal hierarchical assembly of a transcriptionally active chromatin hub containing the ASE and RAG promoters, with Rag2 recruitment and expression dependent on assembly of a functional ASE–Rag1 framework. Finally, we show that signal-dependent down-regulation of RAG gene expression in CD4+CD8+ thymocytes depends on Ikaros and occurs with disassembly of the RAG locus chromatin hub. Our results provide important new insights into the molecular mechanisms that orchestrate RAG gene expression in developing T cells.

Accessibility control of variable region gene assembly during T-cell development

1998 ◽  
Vol 165 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Barry P. Sleckman ◽  
Craig H. Bassing ◽  
Christiana G. Bardon ◽  
Ami Okada ◽  
Bernard Khor ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Development ◽  
Variable Region ◽  
Cell Development ◽  
Gene Assembly ◽  
Region Gene ◽  
Variable Region Gene

scholarly journals Orchestrating T-cell receptor α gene assembly through changes in chromatin structure and organization

2010 ◽  
Vol 49 (1-3) ◽  
pp. 192-201 ◽  
Author(s):  
Han-Yu Shih ◽  
Bingtao Hao ◽  
Michael S. Krangel
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Chromatin Structure ◽  
Cell Receptor ◽  
Gene Assembly

scholarly journals Sequential vs. parallel complexity in simple gene assembly

2008 ◽  
Vol 395 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Miika Langille ◽  
Ion Petre
Keyword(s):  
Gene Assembly ◽  
Parallel Complexity

Comparison of Multiple Gene Assembly Methods for Metabolic Engineering

2007 ◽  
pp. 703-710
Author(s):  
Chenfeng Lu ◽  
Karen Mansoorabadi ◽  
Thomas Jeffries
Keyword(s):  
Metabolic Engineering ◽  
Gene Assembly ◽  
Multiple Gene

Computing Through Gene Assembly

2007 ◽  
pp. 91-105 ◽  
Author(s):  
Tseren-Onolt Ishdorj ◽  
Ion Petre
Keyword(s):  
Gene Assembly
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