Methodology Involved in the Osteogenic Differentiation of Mesenchymal Stem Cells on Chitosan–Collagen Nanofibers Incorporated with Titanium Dioxide Nanoparticles

2019 ◽  
pp. 85-94
Author(s):  
Roqia Ashraf ◽  
Hasham S. Sofi ◽  
Faheem A. Sheikh
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Titanium Dioxide ◽  
Osteogenic Differentiation ◽  
Titanium Dioxide Nanoparticles

Toxic effect of titanium dioxide nanoparticles on human mesenchymal stem cells

2020 ◽  
Vol 16 (3) ◽  
pp. 321-330
Author(s):  
Peralta-Vega Adriana-Berenice ◽  
Parra-Barrera Alberto ◽  
Ramos-Godínez María del Pilar ◽  
López-Marure Rebeca ◽  
Arellano-Galindo José ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Titanium Dioxide ◽  
Toxic Effect ◽  
Titanium Dioxide Nanoparticles ◽  
Human Mesenchymal Stem Cells

scholarly journals Titanium dioxide nanotubes increase purinergic receptor P2Y6 expression and activate its downstream PKCα–ERK1/2 pathway in bone marrow mesenchymal stem cells under osteogenic induction

2021 ◽  
Author(s):  
Chen Wang ◽  
Yanchang Liu ◽  
Xianbo Shang ◽  
Sai Ma ◽  
Huihui Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Titanium Dioxide ◽  
Osteogenic Differentiation ◽  
Purinergic Receptor ◽  
Pure Titanium ◽  
Titanium Implants ◽  
Marrow Mesenchymal Stem Cells ◽  
Osteogenic Effect

Abstract Background Titanium dioxide (TiO2) nanotubes can improve the osseointegration of pure titanium implants, but this exact mechanism has not been fully elucidated. The purinergic receptor P2Y6 is expressed in bone marrow mesenchymal stem cells (BMSCs) and participates in the regulation of bone metabolism. However, it is unclear as to whether P2Y6 is involved in the osteogenic differentiation of BMSCs induced by TiO2 nanotubes. Methods TiO2 nanotubes were prepared on the surface of titanium specimens using the anodizing method. The surface morphology of the nanotubes was observed by a scanning electron microscope, and the elemental analysis was carried out by X-ray energy dispersive spectroscopy. Quantitative reverse transcriptase polymerase chain reaction and western blotting were used to detect the expression of P2Y6, markers of osteogenic differentiation, and PKCα–ERK1/2. Results The average inner diameter of the TiO2 nanotubes increases with an increase in voltage (voltage range of 30–90V), and the expression of P2Y6 in BMSCs could be upregulated by TiO2 nanotubes in osteogenic culture. Inhibition of P2Y6 expression partially inhibited the osteogenic effect of TiO2 nanotubes and downregulated the activity of the PKCα–ERK1/2 pathway. The osteogenic effect of TiO2 nanotubes when combined with P2Y6 agonists was more pronounced. Conclusions TiO2 nanotubes can promote the P2Y6 expression of BMSCs during osteogenic differentiation and promote osteogenesis by activating the PKCα–ERK1/2 pathway.

BMP9 Regulates Osteogenic Differentiation of Mesenchymal Stem Cells Through JNKs Kinase Pathway

2013 ◽  
Vol 40 (12) ◽  
pp. 1220
Author(s):  
Jing XU ◽  
Dan ZHAO ◽  
Jian WANG ◽  
WenJuan WANG ◽  
JinYong LUO
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Kinase Pathway

Wnt7a Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells

2019 ◽  
Author(s):  
Leiluo Yang ◽  
Qing Li ◽  
Junhong Zhang ◽  
Pengcheng Li ◽  
Chaoliang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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