scholarly journals Development of Metabolic Phenotype in Phenylketonuria: Evaluation of the Blaskovics Protein Loading Test at 5 Years of Age

2015 ◽  
pp. 77-84
Author(s):  
P. Burgard ◽  
◽  
E. Mönch ◽  
J. Zschocke ◽  
U. Wendel ◽  
...  
Keyword(s):  
Metabolic Phenotype ◽  
Loading Test ◽  
Protein Loading ◽  
Protein Loading Test

Ultrasonographic Intraparenchymal Renal Resistive Index Variation for Assessing Renal Functional Reserve in Patients Scheduled for Cardiac Surgery: A Pilot Study

2021 ◽  
pp. 1-8
Author(s):  
Sara Samoni ◽  
Gianluca Villa ◽  
Silvia De Rosa ◽  
Faeq Husain-Syed ◽  
Gabriele Guglielmetti ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Pilot Study ◽  
Resistive Index ◽  
Renal Resistive Index ◽  
Roc Curve Analysis ◽  
Loading Test ◽  
Protein Loading ◽  
The Us ◽  
Sensitivity Specificity ◽  
Protein Loading Test

<b><i>Introduction:</i></b> Cardiac surgery-associated acute kidney injury (CSA-AKI) is a common complication in patients undergoing cardiac surgery. Preoperative renal functional reserve (RFR) has been demonstrated to be highly predictive of CSA-AKI. We have previously demonstrated that intraparenchymal renal resistive index variation (IRRIV) measured by ultrasound (US) can identify the presence of RFR in healthy individuals. This study aimed (1) to examine the correlation between the US IRRIV test and RFR measured through the protein loading test in patients undergoing elective cardiac surgery and (2) to determine the value of the 2 methods for predicting occurrence of AKI or subclinical AKI after cardiac surgery. <b><i>Methods:</i></b> Consecutive patients scheduled for cardiac surgery were enrolled for this pilot study. The protein loading test and the IRRIV test were performed in all patients 2 days before cardiac surgery. Correlation between IRRIV and RFR was tested using Pearson correlation analysis. Association between presence of RFR and positive IRRIV test, presence of RFR and AKI and subclinical AKI, and positive IRRIV test and AKI and subclinical AKI was evaluated using logistic regression analysis. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the values of IRRIV for predicting RFR, RFR for predicting AKI and subclinical AKI, and IRRIV for predicting AKI and subclinical AKI. <b><i>Results:</i></b> Among the 31 patients enrolled, significant association was found between IRRIV and RFR (<i>r</i> = 0.81; 95% CI: 0.63–0.90; <i>p</i> &#x3c; 0.01). The association between RFR and IRRIV was described in 27/31 (87.1%) patients. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the IRRIV test were 100, 84, 60, and 100%, respectively. In ROC curve analysis, the area under the curve (AUC) was 0.80 (95% CI: 0.64–0.96). After cardiac surgery, 1/31 (3.2%) patient had AKI and 12/31 (38.7%) had subclinical AKI. RFR predicted subclinical AKI (odds ratio [OR] = 0.93; 95% CI: 0.87–0.98; <i>p</i> = 0.02). The sensitivity, specificity, PPV, and NPV of the RFR were 61, 88.8, 80, and 76%, respectively; the AUC was 0.75 (95% CI: 0.59–0.91). IRRIV predicts subclinical AKI (OR = 0.79; 95% CI: 0.67–0.93; <i>p</i> = 0.005). The sensitivity, specificity, PPV, and NPV of the IRRIV test were 46.1, 100, 100, and 72%, respectively; the AUC was 0.73 (95% CI: 0.58–0.87). <b><i>Conclusion:</i></b> This pilot study suggests that a positive IRRIV test can significantly predict the presence of RFR in patients scheduled for cardiac surgery. RFR measured by the protein loading test or by the US IRRIV test can predict the occurrence of subclinical postoperative AKI. The findings of this study need to be confirmed in large patient cohorts.

Metabolic phenotypes of phenylketonuria. Kinetic and molecular evaluation of the Blaskovics protein loading test

2009 ◽  
Vol 32 (4) ◽  
pp. 506-513 ◽  
Author(s):  
U. Langenbeck ◽  
◽  
P. Burgard ◽  
U. Wendel ◽  
M. Lindner ◽  
...  
Keyword(s):  
Loading Test ◽  
Metabolic Phenotypes ◽  
Protein Loading ◽  
Protein Loading Test ◽  
Molecular Evaluation

Protein-loading test, urinary albumin excretion and renal morphology in diagnosis of subclinical diabetic nephropathy

1992 ◽  
Vol 231 (3) ◽  
pp. 213-217 ◽  
Author(s):  
M. V. SHESTAKOVA ◽  
N. A. MUKHIN ◽  
I.I. DEDOV ◽  
V. N. TITOV ◽  
V. A. WARSHAVSKY
Keyword(s):  
Diabetic Nephropathy ◽  
Urinary Albumin Excretion ◽  
Urinary Albumin ◽  
Loading Test ◽  
Albumin Excretion ◽  
Protein Loading ◽  
Renal Morphology ◽  
Protein Loading Test

STEROID METABOLISM IN THE PERFUSED HUMAN PLACENTA

1978 ◽  
Vol 87 (1) ◽  
pp. 181-191 ◽  
Author(s):  
Alfred S. Wolf ◽  
Klaus A. Musch ◽  
Werner Speidel ◽  
Jürgen R. Strecker ◽  
Christian Lauritzen
Keyword(s):  
Venous Blood ◽  
Placental Lactogen ◽  
Dehydroepiandrosterone Sulphate ◽  
Metabolic Capacity ◽  
Loading Test ◽  
Lower Range ◽  
Group I ◽  
High Concentrations ◽  
Steroid Precursor

ABSTRACT A new model for the perfusion of human term-placentas has been developed for studies on the placental biogenesis of C-18 and C-19 steroids. For viability criteria, the glucose- and oxygen-consumption, regional perfusion control by dye-infusions or scanning after injection of 99Tc-labelled macroparticles, and the histological qualification were chosen. The recycled perfusate was investigated for the steroids oestrone (Oe1), oestradiol-17β (Oe2), oestriol (Oe3), 4-androstene-3,17-dione (A), testosterone (T), and human placental lactogen (HPL) by radioimmunoassay in controls and perfusions with the foetal steroid precursor dehydroepiandrosterone sulphate (DHA-S). In control perfusions, steroid hormones were found in constant ratios (Oe1:Oe2:Oe3:T:A = 30:1.5:100:0.35:1). Following the administration of 10 mg DHA-S for testing the metabolic capacity of the organ, high concentrations of Oe1 (90–720 ng/ml = 250–3970 % as compared to 100% pre-injection values) were found, shortly preceded by a rapid increase of A (66–1000 ng/ml = 100–16 000 %). A typical surge of T (5.3–147 ng/ml = 265–4640 %) preceded the normally slower increment of Oe2 (22–220 ng/ml = 1570–4330 %). The concentrations of Oe3 and HPL remained nearly unchanged. From different steroid patterns after DHA-S-load, two distinct responses of term-placentas could be differentiated: Group I (n=12) showed high concentrations of Oe1 (3200 ± 940 %), a small increase of T (1020 ± 500%), as well as low and delayed values of Oe2 (1660 ± 450%). In Group II (n = 5), values were high for T (3160 ± 1020%) and Oe2 (3300 ± 1110%), whereas Oe1 was found in a lower range (508 ± 302%). In contrast to in vivo findings in maternal venous blood after DHS-S injection to the mother, oestrone was found in perfusions as the main oestrogen fraction from DHA-S. Thus, the analysis of such metabolic differences might be of help in the interpretation of complex results from the DHA-S-loading test.

STEROIDSTOFFWECHSEL BEI BRUSTKREBS. III.

1959 ◽  
Vol XXXII (I) ◽  
pp. 23-32 ◽  
Author(s):  
Kurt Schubert ◽  
Hans Schröder
Keyword(s):  
Advanced Cancer ◽  
Liver Damage ◽  
Adsorption Chromatography ◽  
Hepatic Insufficiency ◽  
Loading Test ◽  
Patients With Cancer ◽  
Urinary Steroids ◽  
Normal Women

ABSTRACT A testosterone test using two different dosages was carried out simultaneously in 7 women suffering from metastasizing carcinoma of the mamma and in 3 normal women. In each case the urinary steroids were estimated before the beginning of the test and after administration of 50 mg and 100 mg of testosterone respectively; the interval between the single estimations being one week. The use of fractionated hydrolysis enabled a mild fission of the conjugates and the classification of the products into free steroids, glucuronosides, sulfates and unknown conjugates. The 17-ketosteroids and the testosterone were estimated by means of Girard's separation and adsorption chromatography. During the loading test with testosterone different behaviours became evident, which had not been realized before. The behaviour of the 17-ketosteroids rendered possible the differentiation of normal women from patients with cancer of the breast yet without hepatic insufficiency, and furthermore of these latter ones from those with a liver damage in addition to the cancer of the breast. The glucuronosides of the 17-ketosteroids are only depressed, when there exists a pronounced damage of the liver; the loading test making possible an extension of the range of recognizable damages. Furthermore, the behaviour of dehydroepiandrosterone (II/III), of androsterone (IV), and of aetiocholanolone (V) lends itself to this differentiation. In advanced cancer of the breast the values of II/III are invariably low, whilst IV and V often increase temporarily. The relation of IV to V may be altered in a different way. The excretion of not transformed testosterone is less in patients than in normal women and especially low in patients with liver damage.

Salivary testosterone to androstenedione (T/A4) ratio is significantly higher in PCOS patients and accompanied with an adverse metabolic phenotype

2016 ◽  
Author(s):  
Julia Muenzker ◽  
Lisa Lindheim ◽  
Jo Adaway ◽  
Elisabeth Lerchbaum ◽  
Thomas Pieber ◽  
...  
Keyword(s):  
Metabolic Phenotype ◽  
Salivary Testosterone

Impact of paternal cholestasis on the metabolic phenotype of female offspring

2016 ◽  
Author(s):  
Vanessa Pataia ◽  
Georgia Papacleovoulou ◽  
Lucilla Poston ◽  
Catherine Williamson
Keyword(s):  
Female Offspring ◽  
Metabolic Phenotype

Oral calcium loading test can predict the progression of hypercalcaemic primary hyperparathyroidism in patients with normocalcaemic hyperparathyroidism

2019 ◽  
Author(s):  
Kanapath Oungpasuk ◽  
Demetrios Hadjiminas ◽  
Neil Tolley ◽  
Stephen Robinson ◽  
Jeremy Cox
Keyword(s):  
Primary Hyperparathyroidism ◽  
Loading Test ◽  
Oral Calcium ◽  
Calcium Loading

2031-P: Adipose-Specific Lack of Hexose-6-Phosphate Dehydrogenase Causes Inactivation of Adipose Glucocorticoids and Improves Metabolic Phenotype in Male Mice

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 2031-P
Author(s):  
JIAN WANG ◽  
XINXIANG WANG ◽  
KABIRULLAH LUTFY ◽  
THEODORE FRIEDMAN ◽  
MEISHENG JIANG ◽  
...  
Keyword(s):  
Metabolic Phenotype ◽  
Male Mice
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