Determination of Mutational Spectra Induced by Environmental Toxicants in Complex Human Cell Populations

2020 ◽  
pp. 303-314
Author(s):  
Robert Gealy ◽  
Phouthone Keohavong
Keyword(s):  
Human Cell ◽  
Cell Populations ◽  
Environmental Toxicants ◽  
Mutational Spectra

Molecular analysis of complex human cell populations: mutational spectra of MNNG and ICR-191

1990 ◽  
Vol 231 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Neal F. Cariello ◽  
Phouthone Keohavong ◽  
Alexandra G. Kat ◽  
William G. Thilly
Keyword(s):  
Molecular Analysis ◽  
Human Cell ◽  
Cell Populations ◽  
Mutational Spectra

Chemical carcinogen alteration of SV40 virus induced transformation of normal human cell populations in vitro

1978 ◽  
Vol 22 (2-3) ◽  
pp. 185-197 ◽  
Author(s):  
George E. Milo ◽  
James R. Blakeslee ◽  
Ronald Hart ◽  
David S. Yohn
Keyword(s):  
Human Cell ◽  
Cell Populations ◽  
Chemical Carcinogen ◽  
Sv40 Virus ◽  
Normal Human Cell ◽  
Normal Human

Label-free RF biosensors for human cell dielectric spectroscopy

2009 ◽  
Vol 1 (6) ◽  
pp. 497-504 ◽  
Author(s):  
Claire Dalmay ◽  
Arnaud Pothier ◽  
Mathilde Cheray ◽  
Fabrice Lalloue ◽  
Marie-Odile Jauberteau ◽  
...  
Keyword(s):  
Human Cell ◽  
Cell Types ◽  
Label Free ◽  
Microwave Frequencies ◽  
Non Invasive ◽  
System Cell ◽  
Electromagnetic Characteristics ◽  
Biosensor Chip ◽  
Rf Signal

This paper presents an original biosensor chip allowing determination of intrinsic relative permittivity of biological cells at microwave frequencies. This sensor permits non-invasive cell identification and discrimination using an RF signal to probe intracellular medium of biological samples. Indeed, these sensors use an RF planar resonator that allows detection capabilities on less than 10 cells, thanks to the microscopic size of its sensitive area. Especially, measurements between 15 and 35 GHz show the ability label-free biosensors to differentiate two human cell types using their own electromagnetic characteristics. The real part of permittivity of cells changes from 20 to 48 for the nervous system cell types studied. The proposed biodetection method is detailed and we show how the accuracy and the repeatability of measurements have been improved to reach reproducible measurements.

scholarly journals Biological strandard for electron microprobe analysis of intracellular sodium concentration.

1976 ◽  
Vol 24 (6) ◽  
pp. 749-751 ◽  
Author(s):  
V S Sottiurai ◽  
R L Malvin ◽  
L F Allard ◽  
W C Bigelow
Keyword(s):  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Large Cell ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Cell Populations ◽  
Intracellular Sodium Concentration ◽  
Saline Solutions

A method is described for the determination of the intracellular concentration of sodium in individual cells using the electron microprobe analyzer. This method gives an accuracy equal to that obtained by using flame photometry on tissues with large cell populations. Intracellular sodium was precipitated in the cell by a fixative containing pyroantimonate. Cartilaginous needles from shark fins which were equilibrated in saline solutions of differing concentrations were used as biological standards.

scholarly journals Differential expression of an equivalent clonotype among BALB/c and C57BL/6 mice

1978 ◽  
Vol 147 (1) ◽  
pp. 1-12 ◽  
Author(s):  
MP Cancro ◽  
NH Sigal ◽  
NR Klinman
Keyword(s):  
Serum Levels ◽  
Inbred Strains ◽  
Cross Reactivity ◽  
Normal Serum ◽  
Cell Populations ◽  
Myeloma Protein ◽  
Regulatory Processes ◽  
Histocompatibility Complex ◽  
Immunoglobulin Allotype

The primary anti-phosphorylcholine (PC) response in BALB/c, C57BL/6, and congenic and recombinant inbred strains of these parental types has been examined in the splenic focus system. The frequencies of PC-specific precursors were shown to vary among these strains from 2 to 20 precursors per 10(6) splenic B cells. The distribution of these frequencies suggests that elements closely linked to or within the major histocompatibility complex may play a role in the determination of this parameter, although additional experiments are necessary to adequately assess this possibility. Moreover, all strains tested, regardless of immunoglobulin allotype, expressed monoclonal antibodies indistinguishable from the TEPC 15 myeloma protein (T15) clonotype. Further, the frequency of this clonotype in a given strain did not appear related to allotype, since both high and low T15 frequencies were found among strains of either the BALB/c (a(1)) or C57BL/6 (a(2)) allotype. The examination of normal serum for the T15 idiotype, however, revealed that only mice of the BALB/c allotype (a(1)) expressed the T15 idiotype in detectable quantities. After immunization with Diplococcus pneumoniae, sera from mice of the a(1) allotype consistently contained large quantities of the T15 idiotype, whereas sera from mice of the a(2) allotype exhibited various degrees of cross-reactivity with anti-T15 antibody. These results suggest that: (a) the allotype of an individual, although closely related to serum levels of an idiotype, is unrelated to the proportion of the precursor population which expresses that idiotype and; (b) the serum expression of a given idiotype may reflect regulatory processes, which act either during or before antigenic stimulation, rather than the actual clonotype representation in the repertoire. These findings indicate that distinctions must be made between the expression of idiotypic determinants within precursor B-cell populations and elements which regulate the subsequent appearance of those idiotypes in serum antibodies.

scholarly journals Dye-coupled electrode system for the rapid determination of cell populations in polluted water.

1982 ◽  
Vol 43 (4) ◽  
pp. 814-818 ◽  
Author(s):  
S Nishikawa ◽  
S Sakai ◽  
I Karube ◽  
T Matsunaga ◽  
S Suzuki
Keyword(s):  
Rapid Determination ◽  
Electrode System ◽  
Polluted Water ◽  
Cell Populations
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