Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein Complexes

2020 ◽  
pp. 17-22 ◽  
Author(s):  
Reka A. Haraszti ◽  
Joerg E. Braun
Keyword(s):  
Single Molecule ◽  
Protein Complexes ◽  
Single Molecule Spectroscopy ◽  
Rna Protein Complexes

scholarly journals Cooperative Analysis of Structural Dynamics in RNA-Protein Complexes by Single-Molecule Förster Resonance Energy Transfer Spectroscopy

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2057 ◽  
Author(s):  
Nathalie Meiser ◽  
Christin Fuks ◽  
Martin Hengesbach
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Protein Complexes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Telomere Maintenance ◽  
Förster Resonance Energy Transfer ◽  
Rna Protein Complexes ◽  
Förster Resonance

RNA-protein complexes (RNPs) are essential components in a variety of cellular processes, and oftentimes exhibit complex structures and show mechanisms that are highly dynamic in conformation and structure. However, biochemical and structural biology approaches are mostly not able to fully elucidate the structurally and especially conformationally dynamic and heterogeneous nature of these RNPs, to which end single molecule Förster resonance energy transfer (smFRET) spectroscopy can be harnessed to fill this gap. Here we summarize the advantages of strategic smFRET studies to investigate RNP dynamics, complemented by structural and biochemical data. Focusing on recent smFRET studies of three essential biological systems, we demonstrate that investigation of RNPs on a single molecule level can answer important functional questions that remained elusive with structural or biochemical approaches alone: The complex structural rearrangements throughout the splicing cycle, unwinding dynamics of the G-quadruplex (G4) helicase RHAU, and aspects in telomere maintenance regulation and synthesis.

Exciton Self Trapping in Photosynthetic Pigment–Protein Complexes Studied by Single-Molecule Spectroscopy

2012 ◽  
Vol 116 (36) ◽  
pp. 11017-11023 ◽  
Author(s):  
Ralf Kunz ◽  
Kõu Timpmann ◽  
June Southall ◽  
Richard J. Cogdell ◽  
Arvi Freiberg ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Complexes ◽  
Photosynthetic Pigment ◽  
Single Molecule Spectroscopy ◽  
Self Trapping ◽  
Pigment Protein Complexes

scholarly journals Energy Transfer in Reconstituted Peridinin-Chlorophyll-Protein Complexes: Ensemble and Single-Molecule Spectroscopy Studies

2007 ◽  
Vol 93 (9) ◽  
pp. 3249-3258 ◽  
Author(s):  
Sebastian Mackowski ◽  
Stephan Wörmke ◽  
Tatas H.P. Brotosudarmo ◽  
Christophe Jung ◽  
Roger G. Hiller ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Protein Complexes ◽  
Single Molecule Spectroscopy ◽  
Chlorophyll Protein Complexes ◽  
Chlorophyll Protein ◽  
Spectroscopy Studies

Single-Molecule Spectroscopy on Photosystem I Pigment−Protein Complexes

2000 ◽  
Vol 104 (34) ◽  
pp. 8093-8096 ◽  
Author(s):  
F. Jelezko ◽  
C. Tietz ◽  
U. Gerken ◽  
J. Wrachtrup ◽  
R. Bittl
Keyword(s):  
Photosystem I ◽  
Single Molecule ◽  
Protein Complexes ◽  
Single Molecule Spectroscopy ◽  
Pigment Protein Complexes

scholarly journals Bringing single-molecule spectroscopy to macromolecular protein complexes

2013 ◽  
Vol 38 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Chirlmin Joo ◽  
Mohamed Fareh ◽  
V. Narry Kim
Keyword(s):  
Single Molecule ◽  
Protein Complexes ◽  
Single Molecule Spectroscopy ◽  
Macromolecular Protein

Single molecule spectroscopy on photosynthetic pigment-protein complexes

2001 ◽  
Vol 91 (3) ◽  
pp. 457-460 ◽  
Author(s):  
F. Jelezko ◽  
C. Tietz ◽  
U. Gerken ◽  
E. Thews ◽  
S. Schuler ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Complexes ◽  
Photosynthetic Pigment ◽  
Single Molecule Spectroscopy ◽  
Pigment Protein Complexes
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