Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors

2020 ◽  
pp. 115-134
Author(s):  
Sofia Golenkina ◽  
Rosemary Manhire-Heath ◽  
Michael J. Murray
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disc ◽  
Wing Imaginal Disc

A genetic and molecular analysis of an invectedDominant mutation in Drosophila melanogaster

Genome ◽  
1998 ◽  
Vol 41 (3) ◽  
pp. 381-390 ◽  
Author(s):  
A J Simmonds ◽  
J B Bell
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disc ◽  
Transcriptional Start Site ◽  
Ectopic Expression ◽  
Careful Analysis ◽  
Wing Imaginal Disc ◽  
Coding Region ◽  
Specific Expression ◽  
Additional Lesion ◽  
Normal Expression

The invected gene of Drosophila melanogaster is a homeobox-containing gene that is closely related to engrailed. A dominant gain of function allele, invectedDominant, was derived from mutagenesis of a dominant allele of vestigial, In(2R)vgW. A careful analysis of the phenotype of invectedDominant shows that it is associated with a transformation of the anterior compartment of the wing to a posterior fate. This transformation is normally limited to the wing blade itself and does not involve the remaining tissues derived from the wing imaginal disc, including the wing hinge and dorsal thorax of the fly. The ectopic expression of invected protein associated with invectedDominant correlates spatially with the normal expression pattern of vestigial in the wing imaginal disc, suggesting that control elements of vestigial are driving ectopic invected expression. This was confirmed by sequence analysis that shows that the dominant vestigial activity was eliminated by a deletion that removes the 3' portion of the vestigial coding region. This leaves a gene fusion wherein the vestigial enhancer elements are still juxtaposed immediately 5' to the invected transcriptional start site, but with the vg sequences harboring an additional lesion. Unlike recessive invected alleles, the invectedDominant allele produces an observable phenotype, and as such, should prove useful in determining the role of invected in patterning the wing imaginal disc. Genetic analysis has shown that mutations of polyhomeotic, a gene involved in regulating engrailed expression, cause a reproducible alteration in the invectedDominant phenotype. Finally, the invectedDominant allele should prove valuable for identifying and characterizing genes that are activated within the posterior compartment. A screen using various lacZ lines that are asymmetrically expressed in an anterior-posterior manner in the wing imaginal disc isolated one line that shows posterior-specific expression within the transformed anterior compartment.Key words: Drosophila, development, dominant mutation, ectopic, wings.

scholarly journals Spalt major controls the development of the notum and of wing hinge primordia of the Drosophila melanogaster wing imaginal disc

2009 ◽  
Vol 329 (2) ◽  
pp. 315-326 ◽  
Author(s):  
Nicole C. Grieder ◽  
Gines Morata ◽  
Markus Affolter ◽  
Walter J. Gehring
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disc ◽  
Wing Imaginal Disc

scholarly journals Essential Roles of the Tap42-Regulated Protein Phosphatase 2A (PP2A) Family in Wing Imaginal Disc Development of Drosophila melanogaster

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e38569 ◽  
Author(s):  
Ning Wang ◽  
Hung-Tat Leung ◽  
Matthew D. Mazalouskas ◽  
Guy R. Watkins ◽  
Rey J. Gomez ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Protein Phosphatase ◽  
Imaginal Disc ◽  
Protein Phosphatase 2A ◽  
Wing Imaginal Disc ◽  
Phosphatase 2A

scholarly journals Role of Jun N-terminal Kinase (JNK) signaling in the wound healing and regeneration of a Drosophila melanogaster wing imaginal disc

2005 ◽  
Vol 49 (4) ◽  
pp. 391-399 ◽  
Author(s):  
Jaakko Mattila ◽  
Leonid Omelyanchuk ◽  
Satu Kyttala ◽  
Heikki Turunen ◽  
Seppo Nokkala
Keyword(s):  
Wound Healing ◽  
Drosophila Melanogaster ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Jnk Signaling

scholarly journals Label-Free 3D Imaging of Development of Cell Patterns in Drosophila melanogaster Wing Imaginal Disc

2011 ◽  
Vol 17 (S2) ◽  
pp. 228-229
Author(s):  
G Rago ◽  
F Marty ◽  
J Day ◽  
R Heeren ◽  
K Basler ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Imaginal Disc ◽  
3D Imaging ◽  
Wing Imaginal Disc ◽  
Label Free ◽  
Cell Patterns

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

scholarly journals The Function of Lipin in the Wing Development of Drosophila melanogaster

2019 ◽  
Vol 20 (13) ◽  
pp. 3288 ◽  
Author(s):  
Tran Duy Binh ◽  
Tuan L. A. Pham ◽  
Taisei Nishihara ◽  
Tran Thanh Men ◽  
Kaeko Kamei
Keyword(s):  
Dna Damage ◽  
Drosophila Melanogaster ◽  
Imaginal Disc ◽  
Cell Cycle Progression ◽  
Apoptotic Cell ◽  
Wing Imaginal Disc ◽  
Wing Development ◽  
Cycle Progression ◽  
Evolutionarily Conserved

Lipin is evolutionarily conserved from yeast to mammals. Although its roles in lipid metabolism in adipocyte tissue, skeletal muscle, and the liver, and as a transcriptional co-activator are known, its functions during development are still under investigation. In this study, we analyzed the role of Drosophila lipin (dLipin) in development. Specifically, we showed that the tissue-selective knockdown of dLipin in the wing pouch led to an atrophied wing. Elevated DNA damage was observed in the wing imaginal disc of dLipin-knockdown flies. dLipin dysfunction induced accumulation of cells in S phase and significantly reduced the number of mitotic cells, indicating DNA damage-induced activation of the G2/M checkpoint. Reduced expression of cyclin B, which is critical for the G2 to M transition, was observed in the margin of the wing imaginal disc of dLipin-knockdown flies. The knockdown of dLipin led to increased apoptotic cell death in the wing imaginal disc. Thus, our results suggest that dLipin is involved in DNA replication during normal cell cycle progression in wing development of Drosophila melanogaster.

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