Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing

2020 ◽  
pp. 179-189
Author(s):  
Kashif Mahfooz ◽  
Tommas J. Ellender
Keyword(s):  
Patch Clamp ◽  
Single Cell ◽  
Rna Sequencing ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Single Cell Rna Sequencing

Whole Cell Patch Clamp of Dispersed Human Islet Cells v1

2021 ◽  
Author(s):  
Xiaoqing Dai ◽  
Austin Bautista ◽  
Patrick E Macdonald
Keyword(s):  
Patch Clamp ◽  
Single Cell ◽  
Pancreatic Islets ◽  
Islet Cells ◽  
Single Cells ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Patch Clamp Electrophysiology ◽  
Channel Currents

Cells use exocytosis to secrete a wide variety of molecules, including proteins, hormones, and neurotransmitters. Exocytosis can be monitored at the single-cell level by using patch-clamp electrophysiology to measure changes in membrane capacitance as vesicles fuse with the cell membrane and release their content. Dispersion of pancreatic islets into single cells allows for individual characterization of electrophysiological characteristics and allows for collection of cellular content for recovery of full-length transcriptomes by use of Smart-seq2. Described in this protocol is the dispersion of pancreatic islets into single cells followed by whole-cell patch clamp electrophysiology which includes parameters representing cell size, exocytosis, sodium channel currents, and calcium channel currents. Cells are then collected individually after recording to be processed for single-cell RNA sequencing.

scholarly journals In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1448
Author(s):  
Asako Noguchi ◽  
Yuji Ikegaya ◽  
Nobuyoshi Matsumoto
Keyword(s):  
Patch Clamp ◽  
Single Cell ◽  
Single Cell Level ◽  
Patch Clamp Recording ◽  
Cell Level ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Recording Techniques

Brain functions are fundamental for the survival of organisms, and they are supported by neural circuits consisting of a variety of neurons. To investigate the function of neurons at the single-cell level, researchers often use whole-cell patch-clamp recording techniques. These techniques enable us to record membrane potentials (including action potentials) of individual neurons of not only anesthetized but also actively behaving animals. This whole-cell recording method enables us to reveal how neuronal activities support brain function at the single-cell level. In this review, we introduce previous studies using in vivo patch-clamp recording techniques and recent findings primarily regarding neuronal activities in the hippocampus for behavioral function. We further discuss how we can bridge the gap between electrophysiology and biochemistry.

Whole-cell patch-clamp recordings from respiratory neurons in neonatal rat brainstem in vitro

1992 ◽  
Vol 134 (2) ◽  
pp. 153-156 ◽  
Author(s):  
Jeffrey C. Smith ◽  
Klaus Ballanyi ◽  
Diethelm W. Richter
Keyword(s):  
Patch Clamp ◽  
Neonatal Rat ◽  
Respiratory Neurons ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp ◽  
Rat Brainstem

Error analysis of Cm measurement under the whole-cell patch-clamp recording

2010 ◽  
Vol 185 (2) ◽  
pp. 307-314 ◽  
Author(s):  
Hao Zhang ◽  
Anlian Qu ◽  
Jie Luo ◽  
Jie Luo
Keyword(s):  
Patch Clamp ◽  
Error Analysis ◽  
Patch Clamp Recording ◽  
Whole Cell ◽  
Cell Patch Clamp ◽  
Whole Cell Patch Clamp
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