Identification of Human Antigen-Specific CD4+ Cells with Peptide-MHC Multimer Technologies

2021 ◽  
pp. 153-163
Author(s):  
I-Ting Chow ◽  
William W. Kwok
Keyword(s):  
Cd4 Cells ◽  
Mhc Multimer

scholarly journals Correction to “Targeting CD4+ Cells with Anti-CD4 Conjugated Mertansine Loaded Nanogels”

2021 ◽  
Vol 22 (3) ◽  
pp. 1323-1323
Author(s):  
Mine Canakci ◽  
Khushboo Singh ◽  
Oyuntuya Munkhbat ◽  
Sudarvili Shanthalingam ◽  
Ankita Mitra ◽  
...  
Keyword(s):  
Cd4 Cells

On-chip micro-biosensor for the detection of human CD4+ cells based on AC impedance and optical analysis

2005 ◽  
Vol 21 (5) ◽  
pp. 696-704 ◽  
Author(s):  
Nirankar N. Mishra ◽  
Scott Retterer ◽  
Thomas J. Zieziulewicz ◽  
Michael Isaacson ◽  
Donald Szarowski ◽  
...  
Keyword(s):  
Ac Impedance ◽  
Optical Analysis ◽  
Cd4 Cells ◽  
On Chip

scholarly journals Cellular cytotoxicity is a form of immunogenic cell death

2020 ◽  
Vol 8 (1) ◽  
pp. e000325 ◽  
Author(s):  
Luna Minute ◽  
Alvaro Teijeira ◽  
Alfonso R Sanchez-Paulete ◽  
Maria C Ochoa ◽  
Maite Alvarez ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Dendritic Cells ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Cd8 T Cells ◽  
Immunogenic Cell Death ◽  
Cell Debris ◽  
Tumor Cell Death ◽  
Mhc Multimer

BackgroundThe immune response to cancer is often conceptualized with the cancer immunity cycle. An essential step in this interpretation is that antigens released by dying tumors are presented by dendritic cells to naive or memory T cells in the tumor-draining lymph nodes. Whether tumor cell death resulting from cytotoxicity, as mediated by T cells or natural killer (NK) lymphocytes, is actually immunogenic currently remains unknown.MethodsIn this study, tumor cells were killed by antigen-specific T-cell receptor (TCR) transgenic CD8 T cells or activated NK cells. Immunogenic cell death was studied analyzing the membrane exposure of calreticulin and the release of high mobility group box 1 (HMGB1) by the dying tumor cells. Furthermore, the potential immunogenicity of the tumor cell debris was evaluated in immunocompetent mice challenged with an unrelated tumor sharing only one tumor-associated antigen and by class I major histocompatibility complex (MHC)-multimer stainings. Mice deficient inBatf3,Ifnar1andSting1were used to study mechanistic requirements.ResultsWe observe in cocultures of tumor cells and effector cytotoxic cells, the presence of markers of immunogenic cell death such as calreticulin exposure and soluble HMGB1 protein. Ovalbumin (OVA)-transfected MC38 colon cancer cells, exogenously pulsed to present the gp100 epitope are killed in culture by mouse gp100-specific TCR transgenic CD8 T cells. Immunization of mice with the resulting destroyed cells induces epitope spreading as observed by detection of OVA-specific T cells by MHC multimer staining and rejection of OVA+EG7 lymphoma cells. Similar results were observed in mice immunized with cell debris generated by NK-cell mediated cytotoxicity. Mice deficient inBatf3-dependent dendritic cells (conventional dendritic cells type 1, cDC1) fail to develop an anti-OVA response when immunized with tumor cells killed by cytotoxic lymphocytes. In line with this, cultured cDC1 dendritic cells uptake and can readily cross-present antigen from cytotoxicity-killed tumor cells to cognate CD8+T lymphocytes.ConclusionThese results support that an ongoing cytotoxic antitumor immune response can lead to immunogenic tumor cell death.

Detection of GAD-Reactive CD4+ Cells in So-Called “Type 1B” Diabetes

2003 ◽  
Vol 1005 (1) ◽  
pp. 378-386 ◽  
Author(s):  
AKIRA SHIMADA ◽  
KEIICHI KODAMA ◽  
JIRO MORIMOTO ◽  
YOICHI OIKAWA ◽  
JUNICHIRO IRIE ◽  
...  
Keyword(s):  
Cd4 Cells

The critical role of mouse CD4+ cells in the rejection of highly disparate xenogeneic pig thymus grafts

2000 ◽  
Vol 7 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Yong Zhao ◽  
Kirsten Swenson ◽  
Thomas Wekerle ◽  
J. I. Rodriguez-Barbosa ◽  
J. Scott Arn ◽  
...  
Keyword(s):  
Critical Role ◽  
Cd4 Cells

scholarly journals Severe feline sporotrichosis associated with an increased population of CD8lowcells and a decrease in CD4+cells

2015 ◽  
pp. myv079 ◽  
Author(s):  
Luisa H. M. Miranda ◽  
Marta de A. Santiago ◽  
Tânia M. P. Schubach ◽  
Fernanda N. Morgado ◽  
Sandro A. Pereira ◽  
...  
Keyword(s):  
Cd4 Cells

Requirement of CD4 cells for induction and maintenance of unresponsiveness in islet xenografted mice

1998 ◽  
Vol 5 (3) ◽  
pp. 207-214 ◽  
Author(s):  
Xiaoshan Lu ◽  
Jean F. Borel
Keyword(s):  
Cd4 Cells
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