Haploid Plant Production in Borage (Borago officinalis L.) by Anther Culture

Author(s):  
Mohammad Reza Abdollahi ◽  
Zahra Chardoli Eshaghi ◽  
Jose M. Seguí-Simarro
1985 ◽  
Vol 65 (4) ◽  
pp. 1033-1037 ◽  
Author(s):  
M. S. CHIANG ◽  
S. FRECHETTE ◽  
C. G. KUO ◽  
CALVIN CHONG ◽  
S. J. DELAFIELD

Thirteen out of nineteen single plant cabbage (Brassica oleracea) selections from a clubroot-resistant breeding population with known variation in glucosinolate composition yielded embryos from cultured anthers. The highest embryo yield was obtained with a line that contained the lowest content of total glucosinolates and which was free of the glucosinolate hydrolysis product, goitrin. Of 191 regenerants examined from five selections, 23 haploids, 105 diploids, 162 tetraploids, and 1 aneuploid were identified.Key words: Tissue culture, glucosinolates, clubroot, cabbage, embryo, haploid


2018 ◽  
Vol 134 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Isidre Hooghvorst ◽  
Eduardo Ramos-Fuentes ◽  
Camilo López-Cristofannini ◽  
Mirari Ortega ◽  
Raimon Vidal ◽  
...  

1993 ◽  
Vol 62 (3) ◽  
pp. 561-565 ◽  
Author(s):  
Kai Lin Hu ◽  
Sachiko Matsubara ◽  
Kenji Murakami

2011 ◽  
Vol 129 (2) ◽  
pp. 335-342 ◽  
Author(s):  
Xiyan Zhang ◽  
Qingqing Wu ◽  
Xiaolu Li ◽  
Sixiang Zheng ◽  
Shimin Wang ◽  
...  

1994 ◽  
Vol 112 (3) ◽  
pp. 192-198 ◽  
Author(s):  
W. Navarro-Alvarez ◽  
P.S. Baenziger ◽  
K.M. Eskridge ◽  
M. Hugo ◽  
V.D. Gustafson

2016 ◽  
Vol 58 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Csaba Lantos ◽  
Barnabás Jenes ◽  
Lajos Bóna ◽  
Mátyás Cserháti ◽  
János Pauk

AbstractThis is the first study to report an efficient anther culture (AC) method for spelt wheat, which has an increasing importance not only in applied research but also in organic farming and changing nutritional standards. In this study, an efficient AC protocol has been described for ‘GK Fehér’ spelt wheat. The number of AC-derived embryo-like structures (ELS) was 62.2/100 anthers, from which we were able to regenerate 30.6 green plantlets per 100 anthers. The percentage of green plantlets production was 89.0% among the regenerated plantlets, while the phenomenon of albinism was restricted (3.8/100 anthers). Altogether, from AC of ‘GK Fehér’ 306 green plantlets were producedin vitroand 241 plants were acclimatized to the greenhouse conditions. Based on ploidy level analyses, 83 spontaneous doubled haploid (DH) plants were produced (8.3 DH plants/100 anthers), so the percentage of spontaneous rediploidization was 34.4%. The spontaneous DH plants produced fertile spikes, while a few seeds were harvested from seven partially fertile plants.


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