Production of Acetone Butanol Ethanol from Degermed Corn Using Clostridium beijerinckii BA101

2002 ◽  
pp. 553-561 ◽  
Author(s):  
Edhilvia J. Campos ◽  
Nasib Qureshi ◽  
Hans P. Blaschek
Keyword(s):  
Clostridium Beijerinckii ◽  
Clostridium Beijerinckii Ba101 ◽  
Acetone Butanol Ethanol

Production of Acetone Butanol Ethanol from Degermed Corn Using Clostridium beijerinckii BA101

2002 ◽  
Vol 98-100 (1-9) ◽  
pp. 553-562 ◽  
Author(s):  
Edhilvia J. Campos ◽  
Nasib Qureshi ◽  
Hans P. Blaschek
Keyword(s):  
Clostridium Beijerinckii ◽  
Clostridium Beijerinckii Ba101 ◽  
Acetone Butanol Ethanol

scholarly journals Production of butyric acid at constant pH by a solventogenic strain of Clostridium beijerinckii

2020 ◽  
Vol 38 (No. 3) ◽  
pp. 185-191 ◽  
Author(s):  
Marek Drahokoupil ◽  
Petra Patáková
Keyword(s):  
Flow Cytometry ◽  
Butyric Acid ◽  
Population Viability ◽  
Clostridium Beijerinckii ◽  
Clostridium Tyrobutyricum ◽  
Fluorescence Staining ◽  
Low Concentration ◽  
Ph Values ◽  
Constant Ph ◽  
Acetone Butanol Ethanol

A solventogenic strain of Clostridium beijerinckii, NRRL B-598, was cultured for the production of butyric acid as the main fermentation product. However, unlike typical acetone-butanol-ethanol (ABE) fermentations, where pH is not regulated, in this study the pH was kept constant during fermentation. From the five pH values tested, 6.0, 6.5, 7.0, 7.5 and 8.0, pH 6.5 and 7.0 resulted in the highest concentrations of butyric acid, at 9.69 ± 0.09 g L–1 and 11.5 ± 0.39 g L–1, respectively. However, a low concentration of solvents, 1.8 ± 0.22 g L–1, was only reached at pH 7.0. These results are comparable with those from typical butyric acid producers, i.e. Clostridium butyricum and Clostridium tyrobutyricum strains. At pH 7.0, we succeeded in suppressing sporulation and prolonging the population viability, which was confirmed by flow cytometry combined with double fluorescence staining.

Production of acetone–butanol–ethanol (ABE) from concentrated yellow top presscake using Clostridium beijerinckii P260

2019 ◽  
Vol 95 (3) ◽  
pp. 614-620 ◽  
Author(s):  
Nasib Qureshi ◽  
Badal Saha ◽  
Siqing Liu ◽  
Rogers Harry‐O'kuru
Keyword(s):  
Clostridium Beijerinckii ◽  
Acetone Butanol Ethanol

scholarly journals Milling byproducts are an economically viable substrate for butanol production using clostridial ABE fermentation

2020 ◽  
Vol 104 (20) ◽  
pp. 8679-8689
Author(s):  
Nils Thieme ◽  
Johanna C. Panitz ◽  
Claudia Held ◽  
Birgit Lewandowski ◽  
Wolfgang H. Schwarz ◽  
...  
Keyword(s):  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Liquid Fuels ◽  
Butanol Production ◽  
Enzymatic Pretreatment ◽  
Profitability Analysis ◽  
Key Points ◽  
Wide Range ◽  
Wheat Middlings ◽  
Acetone Butanol Ethanol

Abstract Butanol is a platform chemical that is utilized in a wide range of industrial products and is considered a suitable replacement or additive to liquid fuels. So far, it is mainly produced through petrochemical routes. Alternative production routes, for example through biorefinery, are under investigation but are currently not at a market competitive level. Possible alternatives, such as acetone-butanol-ethanol (ABE) fermentation by solventogenic clostridia are not market-ready to this day either, because of their low butanol titer and the high costs of feedstocks. Here, we analyzed wheat middlings and wheat red dog, two wheat milling byproducts available in large quantities, as substrates for clostridial ABE fermentation. We could identify ten strains that exhibited good butanol yields on wheat red dog. Two of the best ABE producing strains, Clostridium beijerinckii NCIMB 8052 and Clostridium diolis DSM 15410, were used to optimize a laboratory-scale fermentation process. In addition, enzymatic pretreatment of both milling byproducts significantly enhanced ABE production rates of the strains C. beijerinckii NCIMB 8052 and C. diolis DSM 15410. Finally, a profitability analysis was performed for small- to mid-scale ABE fermentation plants that utilize enzymatically pretreated wheat red dog as substrate. The estimations show that such a plant could be commercially successful. Key points • Wheat milling byproducts are suitable substrates for clostridial ABE fermentation. • Enzymatic pretreatment of wheat red dog and middlings increases ABE yield. • ABE fermentation plants using wheat red dog as substrate are economically viable.

scholarly journals Enhanced robustness in acetone-butanol-ethanol fermentation with engineered Clostridium beijerinckii overexpressing adhE2 and ctfAB

2017 ◽  
Vol 243 ◽  
pp. 1000-1008 ◽  
Author(s):  
Congcong Lu ◽  
Le Yu ◽  
Saju Varghese ◽  
Mingrui Yu ◽  
Shang-Tian Yang
Keyword(s):  
Ethanol Fermentation ◽  
Clostridium Beijerinckii ◽  
Acetone Butanol Ethanol

Butanol production from the effluent of hydrogen fermentation

2011 ◽  
Vol 63 (6) ◽  
pp. 1236-1240 ◽  
Author(s):  
W. H. Chen ◽  
S. Y. Chen ◽  
S. J. Chao ◽  
Z. C. Jian
Keyword(s):  
Consumption Rate ◽  
Clostridium Beijerinckii ◽  
Abe Fermentation ◽  
Acetate Buffer ◽  
Microbial Populations ◽  
Butanol Production ◽  
Practical Application ◽  
Carbohydrate Consumption ◽  
Acetone Butanol Ethanol ◽  
Acetate Butyrate

The purpose of the study was to recover butanol from the effluent of the hydrogen-producing bioreactor containing acetate, butyrate, and carbohydrate. The butanol production by Clostridium beijerinckii NRRL B592 was evaluated under both unsterilized and sterilized conditions for examining the potential of butanol production for the practical application. Sucrose of 10 g/L and butyrate of 2 g/L coupled with acetate buffer were used to mimic the effluent. Sucrose was completely consumed in the both unsterilized and sterilized conditions during acetone-butanol-ethanol (ABE) fermentation. However, the results illustrate that the carbohydrate consumption rate in the unsterilized condition was higher than that in the sterilized condition. The maximum butanol concentrations of 3,500 and 3,750 mg/L were achieved in the sterilized and unsterilized conditions, respectively. Meanwhile, it was found that the acetate and the butyrate concentrations of 600 and 1,500 mg/L, and 300 and 1,000 mg/L were ingested to yield butanol in the sterilized condition and in the unsterilized condition, respectively. The results concluded that high levels of acetate and butyrate could eliminate the interference of other microbial populations, resulting in the enrichment of C. beijerinckii NRRL B592 in the fermentor. The butanol production by C. beijerinckii NRRL B592 could be, therefore, produced from the effluent of the hydrogen-producing bioreactor. It promised that the microbial butanol production is one of attractive bioprocesses to recover energy from wastes.

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