Rhabdoviridae: Rabies and Vesicular Stomatitis Viruses

Two indirect enzyme-linked immunosorbent assays (I-ELISAs) are described for the detection of bovine serum antibody to the New Jersey (NJ) and Indiana (IN) vesicular stomatitis viruses (VSV). Serum samples at a dilution of 1:200 were incubated with binary ethylenimine-inactivated VSV-NJ and VSV-IN type-specific antigens preadsorbed to microtiter plates. Bound antibodies were detected by a murine monoclonal antibody to bovine IgG 1 conjugated with horseradish peroxidase. The performance of each I-ELISA in detecting homotypic and heterotypic antibodies to VSV-NJ and VSV-IN in sequential serum samples from calves experimentally infected with VSV-NJ or VSV-IN was evaluated. The I-ELISAs detected serotype-specific antibodies to either VSV-NJ or VSV-IN in calves infected with the homologous serotype. Homotypic but not heterotypic anti-VSV-NJ antibodies were first demonstrable by the VSV-NJ I-ELISA during the second week postinfection and remained at an elevated level for a period of 11 weeks, with a gradual decrease thereafter. Similar homotypic antibody profiles measured by the VSV-IN I-ELISA in calves inoculated with VSV-IN were observed. The performances of the I-ELISAs were compared using 1,495 microtiter serum neutralization (MTSN) test-negative bovine field sera collected from cattle in Canada (VS free) and 429 samples collected from cattle in the USA and Mexico (VS-epidemic and VS-endemic areas). The diagnostic specificities of the VSV-NJ and VSV-IN I-ELISAs for the Canadian samples relative to the MTSN test results were in the range of 99.8% and 99.7%, respectively. The levels of agreement between the VSV-NJ and VSV-IN I-ELISAs and the MTSN test for the 429 bovine field samples from VS-epidemic and VS-endemic areas were 97.7% and 98.8%, respectively. Relative to MTSN, the sensitivity and specificity of the 429 field sera for the VSV-NJ I-ELISA were 95.4% and 99.6%, respectively, and for the VSV-IN assay were 75.0% and 99.2%, respectively. The results suggest that in addition to the many technical advantages over the MTSN test these I-ELISAs have potential application as rapid and inexpensive tests for the serodiagnosis of VSV infection in cattle.

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Neutralizing Antibodies against Vesicular Stomatitis Viruses (Serotypes New Jersey and Indiana) in Horses in Costa Rica

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870201400517 ◽

2002 ◽

Vol 14 (5) ◽

pp. 438-441 ◽

Cited By ~ 2

Author(s):

Maren Blickwede ◽

Gaby Dolz ◽

Marco V. Herrero ◽

Sarah M. Tomlinson ◽

Mo Salman

Keyword(s):

New Jersey ◽

Costa Rica ◽

Antibody Titer ◽

Neutralizing Antibodies ◽

Serum Samples ◽

Humid Area ◽

Domestic Horses ◽

Vesicular Stomatitis ◽

The Mean ◽

Vesicular Stomatitis Viruses

Serum samples were collected from domestic horses in 4 different regions of Costa Rica to detect antibodies against vesicular stomatitis viruses, serotypes New Jersey (VSV-NJ) and Indiana (VSV-IN). A total of 214 samples were tested by the virus neutralization test. The sampling regions were identified as low North Pacific dry area (1), low Middle Atlantic humid area (2), low South Pacific humid area (3), and the highlands (4). In region 1, 97.1% of horses were positive for VSV-NJ and 16.5% were positive for VSV-IN. The mean antibody titer and its standard deviation after logarithmic transformation were 5.86 ± 0.9 for VSV-NJ and 3.55 ± 1.66 for VSV-IN for region 1. In region 2, 40.7% of horses were positive for VSV-NJ and 32.2% were positive for VSV-IN. The mean antibody titer in region 2 was 4.33 ± 1.82 for VSV-NJ and 3.47 ± 1.73 for VSV-IN. In region 3, 20.79% of horses were positive for VSV-NJ and 27.6% were positive for VSV-IN. The mean antibody titer in region 3 was 4.39 ± 1.89 for VSV-NJ and 3.47 ± 1.82 for VSV-IN. In region 4, 91.3% of horses were positive for VSV-NJ and 73.9% were positive for VSV-IN. The mean antibody titer in region 4 was 5.77 ±1.10 for VSV-NJ and 4.85 ± 1.63 for VSV-IN. This is the first published report of the detection of virus-neutralizing antibodies against VSV-NJ and VSV-IN in horses in Costa Rica.

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