In Vitro Immunization for the Generation of Hybridomas Using Serum-Free Medium

1987 ◽  
pp. 237-248
Author(s):  
Frederick J. Darfler
Keyword(s):  
Serum Free Medium ◽  
Free Medium ◽  
In Vitro Immunization ◽  
Serum Free

Production of murine monoclonal antibodies against human thyroglobulin using an in vitro immunization procedure in serum-free medium

1986 ◽  
Vol 91 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Ferry A. Ossendorp ◽  
Mark De Boer ◽  
Bert J.M. Al ◽  
Jo Hilgers ◽  
Peter F. Bruning ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Serum Free Medium ◽  
Free Medium ◽  
In Vitro Immunization ◽  
Serum Free ◽  
Immunization Procedure ◽  
Murine Monoclonal Antibodies

scholarly journals Quantification of Internalized Silica Nanoparticles via STED Microscopy

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Henrike Peuschel ◽  
Thomas Ruckelshausen ◽  
Christian Cavelius ◽  
Annette Kraegeloh
Keyword(s):  
Silica Nanoparticles ◽  
Super Resolution ◽  
Engineered Nanoparticles ◽  
Stimulated Emission ◽  
Alveolar Type ◽  
Serum Free Medium ◽  
Free Medium ◽  
Essential Information ◽  
Serum Free

The development of safe engineered nanoparticles (NPs) requires a detailed understanding of their interaction mechanisms on a cellular level. Therefore, quantification of NP internalization is crucial to predict the potential impact of intracellular NP doses, providing essential information for risk assessment as well as for drug delivery applications. In this study, the internalization of 25 nm and 85 nm silica nanoparticles (SNPs) in alveolar type II cells (A549) was quantified by application of super-resolution STED (stimulated emission depletion) microscopy. Cells were exposed to equal particle number concentrations (9.2×1010particles mL−1) of each particle size and the sedimentation of particles during exposure was taken into account. Microscopy images revealed that particles of both sizes entered the cells after 5 h incubation in serum supplemented and serum-free medium. According to thein vitrosedimentation, diffusion, and dosimetry (ISDD) model 20–27% of the particles sedimented. In comparison, 102-103NPs per cell were detected intracellularly serum-containing medium. Furthermore, in the presence of serum, no cytotoxicity was induced by the SNPs. In serum-free medium, large agglomerates of both particle sizes covered the cells whereas only high concentrations (≥ 3.8 × 1012particles mL−1) of the smaller particles induced cytotoxicity.

Hybridoma antibody production in vitro in type II serum-free medium using Nutridoma-SP supplements Comparisons with in vivo methods

1991 ◽  
Vol 145 (1-2) ◽  
pp. 213-221 ◽  
Author(s):  
Geneviève Federspiel ◽  
Kenneth C. McCullough ◽  
Ulrich Kihm
Keyword(s):  
Antibody Production ◽  
Type Ii ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

scholarly journals Cytotoxic Effects In Vitro of Highly Purified Streptolysin O on Mouse Macrophages Cultured in a Serum-Free Medium

1966 ◽  
Vol 92 (4) ◽  
pp. 1150-1153 ◽  
Author(s):  
Robert M. Fauve ◽  
Joseph E. Alouf ◽  
Albert Delaunay ◽  
Marcel Raynaud
Keyword(s):  
Serum Free Medium ◽  
Free Medium ◽  
Cytotoxic Effects ◽  
Serum Free ◽  
Streptolysin O ◽  
Mouse Macrophages

Innovative serum-free medium for in vitro cultivation of promastigote forms of Leishmania species

2008 ◽  
Vol 136 ◽  
pp. S150
Author(s):  
Abdalla Hassan Sharief ◽  
Eltahir A. Khalil ◽  
Samia A. Omer ◽  
Hamid S. Abdalla
Keyword(s):  
Leishmania Species ◽  
In Vitro Cultivation ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free

In Vitro Islet Culture: Development of a Serum-Free Medium

1998 ◽  
Vol 30 (2) ◽  
pp. 379 ◽  
Author(s):  
H.A Clayton ◽  
Y.M Goward ◽  
P Prevost ◽  
S.M Swift ◽  
D Kimber ◽  
...  
Keyword(s):  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Islet Culture ◽  
Culture Development
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