Use of Two Monoclonal Anti-Human B Cell Antibodies in the Study of Early B Cell Differentiation

1986 ◽  
pp. 87-95
Author(s):  
Anand Raghavachar ◽  
Shraga F. Goldmann ◽  
Bernhard Kubanek
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
Human B Cell

Induction of human B cell differentiation by Fc region activators

1989 ◽  
Vol 50 (2) ◽  
pp. 251-263 ◽  
Author(s):  
Monte V. Hobbs ◽  
Richard A. Houghten ◽  
Jodee A. Janda ◽  
William O. Weigle ◽  
Edward L. Morgan
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
Human B Cell

Molecular structure and immunological function of human B cell differentiation factor (BSF2)

1987 ◽  
pp. 123-136 ◽  
Author(s):  
Tadamitsu Kishimoto ◽  
Tetsuya Taga ◽  
Kiyoshi Yasukawa ◽  
Yasuo Watanabe ◽  
Tadashi Matsuda ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
Immunological Function ◽  
Differentiation Factor ◽  
Cell Differentiation Factor ◽  
Human B Cell

scholarly journals Optimized Protocols for in Vitro T Cell-Dependent and T Cell-Independent Activation for B Cell Differentiation Studies Using Limited Cells

2021 ◽  
Author(s):  
Casper Marsman ◽  
Dorit Verhoeven
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
B Cells ◽  
B Cell ◽  
Plasma Cells ◽  
Cell Formation ◽  
B Cell Differentiation ◽  
Differentiation Potential ◽  
Human B Cell

Background/methods: For mechanistic studies, in vitro human B cell differentiation and generation of plasma cells are invaluable techniques. However, the heterogeneity of both T cell-dependent (TD) and T cell-independent (TI) stimuli and the disparity of culture conditions used in existing protocols makes interpretation of results challenging. The aim of the present study was to achieve the most optimal B cell differentiation conditions using isolated CD19+ B cells and PBMC cultures. We addressed multiple seeding densities, different durations of culturing and various combinations of TD stimuli and TI stimuli including B cell receptor (BCR) triggering. B cell expansion, proliferation and differentiation was analyzed after 6 and 9 days by measuring B cell proliferation and expansion, plasmablast and plasma cell formation and immunoglobulin (Ig) secretion. In addition, these conditions were extrapolated using cryopreserved cells and differentiation potential was compared. Results: This study demonstrates improved differentiation efficiency after 9 days of culturing for both B cell and PBMC cultures using CD40L and IL-21 as TD stimuli and 6 days for CpG and IL-2 as TI stimuli. We arrived at optimized protocols requiring 2500 and 25.000 B cells per culture well for TD and TI assays, respectively. The results of the PBMC cultures were highly comparable to the B cell cultures, which allows dismissal of additional B cell isolation steps prior to culturing. In these optimized TD conditions, the addition of anti-BCR showed little effect on phenotypic B cell differentiation, however it interferes with Ig secretion measurements. Addition of IL-4 to the TD stimuli showed significantly lower Ig secretion. The addition of BAFF to optimized TI conditions showed enhanced B cell differentiation and Ig secretion in B cell but not in PBMC cultures. With this approach, efficient B cell differentiation and Ig secretion was accomplished when starting from fresh or cryopreserved samples. Conclusion: Our methodology demonstrates optimized TD and TI stimulation protocols for more indepth analysis of B cell differentiation in primary human B cell and PBMC cultures while requiring low amounts of B cells, making them ideally suited for future clinical and research studies on B cell differentiation of patient samples from different cohorts of B cell-mediated diseases.

Human B-cell differentiation induced by murein

1985 ◽  
Vol 10 (5) ◽  
pp. 293-296 ◽  
Author(s):  
J. Lüdemann ◽  
W.L. Gross ◽  
W. Bessler ◽  
V. Braun
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
Human B Cell

scholarly journals THU0243 ROLE OF METHIONINE AND ITS TRANSPORTER CD98 IN HUMAN B CELL DIFFERENTIATION AND THE RELEVANCE TO PATHOLOGICAL PROCESSES OF SLE

2019 ◽  
Author(s):  
Mingzeng Zhang ◽  
Shigeru Iwata ◽  
Maiko Hajime ◽  
Naoaki Ohkubo ◽  
Yasuyuki Todoroki ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
B Cell Differentiation ◽  
Human B Cell

Anti-CD3 antibodies induce T helper function for human B cell differentiation in vitro by an interleukin 2-independent pathway

1988 ◽  
Vol 18 (1) ◽  
pp. 133-137 ◽  
Author(s):  
Robert W. Sauerwein ◽  
Walter G. J. Der Van Meer ◽  
Johan W. Van Oostveen ◽  
Frank Miedema ◽  
Lucien A. Aarden
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
Interleukin 2 ◽  
B Cell Differentiation ◽  
T Helper ◽  
Helper Function ◽  
Human B Cell

scholarly journals The Expression of p18INK4and p27kip1Cyclin-Dependent Kinase Inhibitors Is Regulated Differently During Human B Cell Differentiation

2000 ◽  
Vol 165 (8) ◽  
pp. 4346-4352 ◽  
Author(s):  
Nicolas Schrantz ◽  
Georges Edouard Beney ◽  
Marie Thérèse Auffredou ◽  
Marie Françoise Bourgeade ◽  
Gérald Leca ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
B Cell ◽  
Kinase Inhibitors ◽  
B Cell Differentiation ◽  
Human B Cell
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