Phosphotyrosyl Peptide-Enzyme Complexes: How Much Structure Can We Get From Transferred Noe’s

1996 ◽  
pp. 91-99
Author(s):  
Carol Beth Post ◽  
Michael L. Schneider
Keyword(s):  
Enzyme Complexes

Enzyme-assisted Extraction for Optimized Recovery of Phenolic Bioactives from Peganum hermala Leaves Using Response Surface Methodology

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Coumaric Acid ◽  
Assisted Extraction ◽  
Pre Treatment ◽  
Optimized Conditions ◽  
Enzyme Complexes ◽  
Hydrolysis Of ◽  
Central Composite

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.

scholarly journals Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Dongdong Meng ◽  
Ranran Wu ◽  
Juan Wang ◽  
Zhiguang Zhu ◽  
Chun You
Keyword(s):  
Reaction Rate ◽  
Specific Interaction ◽  
Renewable Resource ◽  
Cellulosic Biomass ◽  
Initial Reaction ◽  
Enzyme Complex ◽  
Bioelectricity Generation ◽  
Favorable Reaction ◽  
Enzyme Complexes

Abstract Background Cellulosic biomass, the earth’s most abundant renewable resource, can be used as substrates for biomanufacturing biofuels or biochemicals via in vitro synthetic enzymatic biosystems in which the first step is the enzymatic phosphorolysis of cellodextrin to glucose 1-phosphate (G1P) by cellodextrin phosphorylase (CDP). However, almost all the CDPs prefer cellodextrin synthesis to phosphorolysis, resulting in the low reaction rate of cellodextrin phosphorolysis for biomanufacturing. Results To increase the reaction rate of cellodextrin phosphorolysis, synthetic enzyme complexes containing CDP and phosphoglucomutase (PGM) were constructed to convert G1P to glucose 6-phosphate (G6P) rapidly, which is an important intermediate for biomanufacturing. Four self-assembled synthetic enzyme complexes were constructed with different spatial organizations based on the high-affinity and high-specific interaction between cohesins and dockerins from natural cellulosomes. Thus, the CDP–PGM enzyme complex with the highest enhancement of initial reaction rate was integrated into an in vitro synthetic enzymatic biosystem for generating bioelectricity from cellodextrin. The in vitro biosystem containing the best CDP–PGM enzyme complex exhibited a much higher current density (3.35-fold) and power density (2.14-fold) than its counterpart biosystem containing free CDP and PGM mixture. Conclusions Hereby, we first reported bioelectricity generation from cellulosic biomass via in vitro synthetic enzymatic biosystems. This work provided a strategy of how to link non-energetically favorable reaction (cellodextrin phosphorolysis) and energetically favorable reaction (G1P to G6P) together to circumvent unfavorable reaction equilibrium and shed light on improving the reaction efficiency of in vitro synthetic enzymatic biosystems through the construction of synthetic enzyme complexes.

scholarly journals Photoactive Protochlorophyllide-Enzyme Complexes Reconstituted with PORA, PORB and PORC Proteins of A. thaliana: Fluorescence and Catalytic Properties

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0116990 ◽  
Author(s):  
Michał Gabruk ◽  
Anna Stecka ◽  
Wojciech Strzałka ◽  
Jerzy Kruk ◽  
Kazimierz Strzałka ◽  
...  
Keyword(s):  
Catalytic Properties ◽  
Enzyme Complexes

scholarly journals Functional role of the MrpA- and MrpD-homologous protein subunits in enzyme complexes evolutionary related to respiratory chain complex I

2014 ◽  
Vol 1837 (1) ◽  
pp. 178-185 ◽  
Author(s):  
Vamsi K. Moparthi ◽  
Brijesh Kumar ◽  
Yusra Al-Eryani ◽  
Eva Sperling ◽  
Kamil Górecki ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Complex I ◽  
Functional Role ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Protein Subunits ◽  
Homologous Protein ◽  
Enzyme Complexes

scholarly journals A20 inhibition of NFκB and inflammation: Targeting E2:E3 ubiquitin enzyme complexes

Cell Cycle ◽  
2010 ◽  
Vol 9 (13) ◽  
pp. 2481-2482 ◽  
Author(s):  
Noula Shembade ◽  
Edward Harhaj
Keyword(s):  
Inflammation Targeting ◽  
Enzyme Complexes
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