Nitric Oxide, an Intermediate in the Denitrification Process and Other Bacterial Pathways, as Detected by EPR Spectroscopy

1997 ◽  
pp. 145-173 ◽  
Author(s):  
Yann A. Henry
Keyword(s):  
Nitric Oxide ◽  
Epr Spectroscopy ◽  
Denitrification Process

New insights into the activity of Pseudomonas aeruginosa cd1 nitrite reductase

2008 ◽  
Vol 36 (6) ◽  
pp. 1155-1159 ◽  
Author(s):  
Serena Rinaldo ◽  
Alessandro Arcovito ◽  
Giorgio Giardina ◽  
Nicoletta Castiglione ◽  
Maurizio Brunori ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Nitrite Reductase ◽  
Nitrite Reduction ◽  
Nitrite Reductases ◽  
Cytochrome Cd1 ◽  
Haem Iron ◽  
Shed Light ◽  
Denitrification Process

The cytochrome cd1 nitrite reductases are enzymes that catalyse the reduction of nitrite to nitric oxide (NO) in the bacterial energy conversion denitrification process. These enzymes contain two different redox centres: one covalently bound c-haem, which is reduced by external donors, and one peculiar d1-haem, where catalysis occurs. In the present paper, we summarize the current understanding of the reaction of nitrite reduction in the light of the most recent results on the enzyme from Pseudomonas aeruginosa and discuss the differences between enzymes from different organisms. We have evidence that release of NO from the ferrous d1-haem occurs rapidly enough to be fully compatible with the turnover, in contrast with previous hypotheses, and that the substrate nitrite is able to displace NO from the d1-haem iron. These results shed light on the mechanistic details of the activity of cd1 nitrite reductases and on the biological role of the d1-haem, whose presence in this class of enzymes has to date been unexplained.

Increased synthesis of nitric oxide in rat brain cortex due to halogenated volatile anesthetics confirmed by EPR spectroscopy

2002 ◽  
Vol 46 (4) ◽  
pp. 378-383 ◽  
Author(s):  
L. Baumane ◽  
M. Dzintare ◽  
L. Zvejniece ◽  
D. Meirena ◽  
L. Lauberte ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Rat Brain ◽  
Epr Spectroscopy ◽  
Brain Cortex ◽  
Volatile Anesthetics ◽  
Rat Brain Cortex

Sensitive quantitation of nitric oxide by EPR spectroscopy

1996 ◽  
Vol 21 (5) ◽  
pp. 733-737 ◽  
Author(s):  
Koichiro Tsuchiya ◽  
Masumitsu Takasugi ◽  
Kazuo Minakuchi ◽  
Kenji Fukuzawa
Keyword(s):  
Nitric Oxide ◽  
Epr Spectroscopy

In vivo three-dimensional EPR imaging of nitric oxide production from isosorbide dinitrate in mice

1998 ◽  
Vol 274 (5) ◽  
pp. G857-G862 ◽  
Author(s):  
Satoshi Fujii ◽  
Yasuhiro Suzuki ◽  
Tetsuhiko Yoshimura ◽  
Hitoshi Kamada
Keyword(s):  
Nitric Oxide ◽  
Isosorbide Dinitrate ◽  
Epr Spectroscopy ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Water Soluble ◽  
Whole Body ◽  
No Production ◽  
Epr Signal

Recently, in vivo electron paramagnetic resonance (EPR) spectroscopy and imaging have been widely used to investigate free radical distribution and metabolism in tissues, organs, and whole body of small animals. Endogenous nitric oxide (NO) is an attractive target of this method. In the present study, NO production from a nitrovasodilator, isosorbide dinitrate (ISDN), in live mice was investigated by in vivo EPR spectroscopy and imaging combined with the spin-trapping technique. A highly water-soluble Fe complex with N-(dithiocarboxy)sarcosine (DTCS) was used as an NO-trapping agent. Mice received [14N]ISDN, and the Fe-DTCS complex subcutaneously exhibited the characteristic triplet EPR signal of the NO adduct [14NO-Fe(DTCS)2]2−. Using [15N]ISDN instead of [14N]ISDN, we were able to observe that the doublet EPR signal stemmed from the15NO adduct, which directly demonstrated that NO was produced from ISDN. The three-dimensional EPR images of the upper abdomen of living mice showed that the NO adducts were distributed in the liver and the kidneys. This EPR image combined with the ex vivo EPR measurements of the blood suggested that NO production from ISDN occurred in the liver in this experimental condition.

Nitric oxide Versus Reactive Oxygen Species Measurements in Blood using EPR Spectroscopy for Assessment of Endothelial Dysfunction

2013 ◽  
Vol 65 ◽  
pp. S103-S104
Author(s):  
Bartosz Proniewski ◽  
Antonina Chmura-Skirliñska ◽  
Agnieszka Broniec ◽  
Ryszard J Gurbiel ◽  
Wojciech Froncisz
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Endothelial Dysfunction ◽  
Epr Spectroscopy ◽  
Reactive Oxygen ◽  
Oxygen Species

Reaction of Substituted Anthracenes and a Butadiene with Nitric Oxide: Product Formation Determined by EPR Spectroscopy

1997 ◽  
Vol 27 (4) ◽  
pp. 377-388 ◽  
Author(s):  
Dietmar Pfeiler ◽  
I. Darren Grice ◽  
Steven E. Bottle ◽  
Graeme R. Hanson
Keyword(s):  
Nitric Oxide ◽  
Epr Spectroscopy ◽  
Product Formation ◽  
Oxide Product
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