Evidence of Exposure of Fish to Produced Water at Three Offshore Facilities, North West Shelf, Australia

Abstract Severe fouling of crude oil and produced water treatment equipment of Mangala Processing Terminal (MPT) with elastic deposits has been observed after EOR polymer breaking through to the producing wells. Fouling by polymer containing solids caused the system bottlenecking impacting on crude production rates and deterioration of water quality for injection due to increase of total solids loading. The objective of the study included developing the water treatment technology for removing the returned polymer, developing the pilot run for implementation of the technology and scaling up the process if the pilot shows success. Crude processed at MPT is produced from Mangala, Bhagyam and Aishwarya fields which are located at the north-west part of India. Full field polymer flooding has been implemented in the Mangala field from 2015. Fouling of downhole and topside equipment with elastic deposits has been reported soon after polymer breakthrough the same year. For reducing the fouling potential and solids loading, the concept of removing the returned polymer from produced water has been considered as beneficial. Removal of polymer through the chemical coagulation was considered for developing. Extensive laboratory and bench testing have been carried out. Based on the laboratory results, the pilot was developed and carried out on the flotation equipment available at MPT. In the laboratory and bench test for polymer coagulation, over 70% polymer removal was achieved with non-sticky flocks and minimal sludge. The tests also demonstrated reduced suspended solids, residual oil and filterability improvement of treated water. The pilot run confirmed effectiveness of the chemical coagulation process to remove polymer. Polymer removal > 70% was observed during the pilot. Oil removal from produced water at 60-80% was seen. Cloud point of polymer remaining in water increased from 60°C to > 110°C indicating on the significant potential reduction of remaining polymer to precipitate from treated water. The pilot results demonstrated on the applicability of the technology of chemical polymer removal at MPT and will be used for scaling up the treatment facilities.

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Electron Microscopic Examination of a Transplantable Rat Mammary Carcinoma

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099842 ◽

1968 ◽

Vol 26 ◽

pp. 20-21

Author(s):

S. Shirahama ◽

G. C. Engle ◽

R. M. Dutcher

Keyword(s):

Electron Microscope ◽

Osmium Tetroxide ◽

Electron Microscopic Examination ◽

Undifferentiated Carcinoma ◽

Uranyl Acetate ◽

Sprague Dawley ◽

Electron Microscopic ◽

North West ◽

X Irradiation ◽

Tissue Specimens

A transplantable carcinoma was established in North West Sprague Dawley (NWSD) rats by use of X-irradiation by Engle and Spencer. The tumor was passaged through 63 generations over a period of 32 months. The original tumor, an adenocarcinoma, changed into an undifferentiated carcinoma following the 19th transplant. The tumor grew well in NWSD rats of either sex at various ages. It was invariably fatal, causing death of the host within 15 to 35 days following transplantation.Tumor, thymus, spleen, and plasma from 7 rats receiving transplants of tumor at 3 to 9 weeks of age were examined with an electron microscope at intervals of 8, 15, 22 and 30 days after transplantation. Four normal control rats of the same age were also examined. The tissues were fixed in glutaraldehyde, postfixed in osmium tetroxide and embedded in Epon. The plasma was separated from heparanized blood and processed as previously described for the tissue specimens. Sections were stained with uranyl acetate followed by lead citrate and examined with an RCA EMU-3G electron microscope.

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SEM and TEM observations of sperm development in the testis of the freshwater fish Oreochromis mossambicus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157838 ◽

1990 ◽

Vol 48 (3) ◽

pp. 58-59

Author(s):

Daryl A. Cornish ◽

George L. Smit

Keyword(s):

Morphological Characteristics ◽

Oreochromis Mossambicus ◽

Breeding Cycle ◽

Limited Information ◽

North West ◽

The North ◽

Sem And Tem ◽

Sperm Development ◽

Ultrastructural Characteristics ◽

The University

Oreochromis mossambicus is currently receiving much attention as a candidater species for aquaculture programs within Southern Africa. This has stimulated interest in its breeding cycle as well as the morphological characteristics of the gonads. Limited information is available on SEM and TEM observations of the male gonads. It is known that the testis of O. mossambicus is a paired, intra-abdominal structure of the lobular type, although further details of its characteristics are not known. Current investigations have shown that spermatids reach full maturity some two months after the female becomes gravid. Throughout the year, the testes contain spermatids at various stages of development although spermiogenesis appears to be maximal during November when spawning occurs. This paper describes the morphological and ultrastructural characteristics of the testes and spermatids.Specimens of this fish were collected at Syferkuil Dam, 8 km north- west of the University of the North over a twelve month period, sacrificed and the testes excised.

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