Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes

2012 ◽  
pp. 505-517 ◽  
Author(s):  
Clement K. M. Tsui ◽  
Bin Wang ◽  
Cor D. Schoen ◽  
Richard C. Hamelin
Keyword(s):  
Pathogenic Fungi ◽  
Rapid Identification ◽  
Padlock Probes

Molecular identification of Neofabraea species associated with bull’s-eye rot on apple using rolling-circle amplification of partial EF-1α sequence

2018 ◽  
Vol 64 (1) ◽  
pp. 57-68 ◽  
Author(s):  
Huijiao Lin ◽  
Xiang Jiang ◽  
Jianping Yi ◽  
Xinguo Wang ◽  
Ranling Zuo ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Pathogenic Fungi ◽  
Epidemiological Studies ◽  
Rapid Identification ◽  
Target Species ◽  
Probe Signal ◽  
Closely Related Species ◽  
Rolling Circle ◽  
Padlock Probes ◽  
Bull's Eye

A rolling-circle amplification (RCA) method with padlock probes targeted on EF-1α regions was developed for rapid detection of apple bull’s-eye rot pathogens, including Neofabraea malicorticis, N. perennans, N. kienholzii, and N. vagabunda (synonym: N. alba). Four padlock probes (PLP-Nm, PLP-Np, PLP-Nk, and PLP-Nv) were designed and tested against 28 samples, including 22 BER pathogen cultures, 4 closely related species, and 2 unrelated species that may cause serious apple decays. The assay successfully identified all the bull’s-eye rot pathogenic fungi at the level of species, while no cross-reaction was observed in all target species and no false-positive reaction was observed with all strains used for reference. This study showed that the use of padlock probes and the combination of probe signal amplification by RCA provided an effective and sensitive method for the rapid identification of Neofabraea spp. The method could therefore be a useful tool for monitoring bull’s-eye rot pathogens in port quarantine and orchard epidemiological studies.

Rapid identification and detection of pine pathogenic fungi associated with mountain pine beetles by padlock probes

2010 ◽  
Vol 83 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Clement K.M. Tsui ◽  
Bin Wang ◽  
Lily Khadempour ◽  
Sepideh Massoumi Alamouti ◽  
Jörg Bohlmann ◽  
...  
Keyword(s):  
Pathogenic Fungi ◽  
Rapid Identification ◽  
Mountain Pine Beetles ◽  
Padlock Probes ◽  
Mountain Pine ◽  
Pine Beetles

scholarly journals Correction to: Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes

2018 ◽  
Vol 183 (4) ◽  
pp. 737-737
Author(s):  
M. J. Najafzadeh ◽  
V. A. Vicente ◽  
Peiying Feng ◽  
A. Naseri ◽  
Jiufeng Sun ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Padlock Probes ◽  
Exophiala Species

scholarly journals Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes

2018 ◽  
Vol 183 (4) ◽  
pp. 669-677 ◽  
Author(s):  
M. J. Najafzadeh ◽  
V. A. Vicente ◽  
Peiying Feng ◽  
A. Naseri ◽  
Jiufeng Sun ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Padlock Probes ◽  
Exophiala Species

scholarly journals Specific immunological test for the rapid identification of members of the genus Histoplasma

1976 ◽  
Vol 3 (2) ◽  
pp. 191-199
Author(s):  
P G Standard ◽  
L Kaufman
Keyword(s):  
Fungal Pathogens ◽  
Histoplasma Capsulatum ◽  
Brain Heart Infusion ◽  
Pathogenic Fungi ◽  
Rapid Identification ◽  
In Vivo Studies ◽  
Immunological Test ◽  
Chrysosporium Keratinophilum ◽  
Culture Supernatants

A sensitive and specific immunological method was developed for rapid identification of the mycelial forms of Histoplasma capsulatum var. capsulatum, H. capsulatum var. duboisii, and H. farciminosum and for separation of these pathogenic fungi from morphologically similar hyphomycetes and other fungal pathogens. This method is based on the fact that all of the Histoplasma spp. produce H and M histoplasmin antigens, whereas the other fungi do not. Inocula consisting of heavy mycelial growth from a pure, full-grown culture were transferred into flasks containing small volumes of brain heart infusion broth. These cultures were placed on a shaker and grown at 25 C. Using the micro-immunodiffusion technique and antisera containing antibodies to H and M precipitinogens, we detected exoantigens in 3-day-old brain heart infusion culture supernatants concentrated 25 and 50 times. The ability of the procedure to identify Histoplasma spp. was evaluated by testing 96 unknown mycelial cultures that grossly or microscopically resembled Histoplasma spp. Three- and six-day-old concentrated culture supernatants prepared from each unknown were tested against rabbit anti-Arthroderma tuberculatum, Chrysosporium keratinophilum, H. capsulatum var. duboisii, and Corynascus (Thielavia) sepedonium sera and human histoplasmosis case serum. Each unknown was also identified by conventional laboratory procedures involving cultural and, where necessary, in vivo studies. In the comparative evaluation the immunological test was observed to be 100% sensitive. It permitted the accurate generic identification of the Histoplasma spp. within 5 days, in contrast to the average of 33 days required by the routine mycological procedure.

Multiplex and quantifiable detection of nucleic acid from pathogenic fungi using padlock probes, generic real time PCR and specific suspension array readout

2009 ◽  
Vol 78 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Ronnie Eriksson ◽  
Magnus Jobs ◽  
Charlotta Ekstrand ◽  
Måns Ullberg ◽  
Björn Herrmann ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Pathogenic Fungi ◽  
Suspension Array ◽  
Padlock Probes

Endovascular management of ruptured abdominal aortic aneurysms and acute aortic dissections

VASA ◽  
2019 ◽  
Vol 48 (1) ◽  
pp. 35-46
Author(s):  
Stephen Hofmeister ◽  
Matthew B. Thomas ◽  
Joseph Paulisin ◽  
Nicolas J. Mouawad
Keyword(s):  
Abdominal Aortic Aneurysms ◽  
Rapid Identification ◽  
Aortic Aneurysms ◽  
Endovascular Interventions ◽  
Current State ◽  
Technological Advances ◽  
Abdominal Aortic ◽  
Ruptured Abdominal Aortic Aneurysms ◽  
Vascular Emergencies ◽  
Aortic Dissections

Abstract. The management of vascular emergencies is dependent on rapid identification and confirmation of the diagnosis with concurrent patient stabilization prior to immediate transfer to the operating suite. A variety of technological advances in diagnostic imaging as well as the advent of minimally invasive endovascular interventions have shifted the contemporary treatment algorithms of such pathologies. This review provides a comprehensive discussion on the current state and future trends in the management of ruptured abdominal aortic aneurysms as well as acute aortic dissections.

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