Vascular and Endothelial Regeneration

2013 ◽  
pp. 157-166
Author(s):  
Louis Casteilla ◽  
Patrick Laharrague ◽  
V. Planat-Benard
Keyword(s):  
Endothelial Regeneration

scholarly journals Silk Fibroin Films for Corneal Endothelial Regeneration: Transplant in a Rabbit Descemet Membrane Endothelial Keratoplasty

2017 ◽  
Vol 58 (9) ◽  
pp. 3357 ◽  
Author(s):  
Natalia Vázquez ◽  
Carlos A. Rodríguez-Barrientos ◽  
Salvador D. Aznar-Cervantes ◽  
Manuel Chacón ◽  
José L. Cenis ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Endothelial Keratoplasty ◽  
Descemet Membrane Endothelial Keratoplasty ◽  
Descemet Membrane ◽  
Endothelial Regeneration

scholarly journals Effect of gene delivery of NOS isoforms on intimal hyperplasia and endothelial regeneration after balloon injury

Gene Therapy ◽  
2006 ◽  
Vol 14 (5) ◽  
pp. 396-404 ◽  
Author(s):  
R Cooney ◽  
S O Hynes ◽  
F Sharif ◽  
L Howard ◽  
T O'Brien
Keyword(s):  
Gene Delivery ◽  
Intimal Hyperplasia ◽  
Balloon Injury ◽  
Endothelial Regeneration ◽  
Nos Isoforms

scholarly journals Smooth Muscle Contact Drives Endothelial Regeneration by BMPR2-Notch1–Mediated Metabolic and Epigenetic Changes

2019 ◽  
Vol 124 (2) ◽  
pp. 211-224 ◽  
Author(s):  
Kazuya Miyagawa ◽  
Minyi Shi ◽  
Pin-I Chen ◽  
Jan K. Hennigs ◽  
Zhixin Zhao ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Epigenetic Changes ◽  
Endothelial Regeneration

scholarly journals Cell Based Therapy for Corneal Endothelial Regeneration

2019 ◽  
Vol 97 (S263) ◽  
Author(s):  
Sana Niazi ◽  
Azad Sanginabadi
Keyword(s):  
Endothelial Regeneration ◽  
Cell Based Therapy

scholarly journals Circulating stem and progenitor cells as markers of inflammation, endothelial regeneration, and prediction of vascular complications in metabolic syndrome and type 1 and 2 diabetes mellitus

2018 ◽  
pp. 58-67
Author(s):  
О.В. Першина ◽  
А.В. Пахомова ◽  
Н.Н. Ермакова ◽  
О.Ю. Рыбалкина ◽  
В.А. Крупин ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Metabolic Syndrome ◽  
Stem Cells ◽  
Bone Marrow ◽  
Endothelial Cells ◽  
Progenitor Cells ◽  
Vascular Complications ◽  
Endothelial Regeneration

Цель исследования состояла в выявлении информативных клеточных маркеров сосудистых осложнений, регенерации микрососудистой сети и воспаления в венозной крови здоровых волонтеров, больных с метаболическим синдромом, сахарным диабетом 1 и 2 типа. Методы. Обследованы больные с метаболическим синдромом (МС), диабетом 2 типа без осложнений, диабетом 1 типа средней степени тяжести и здоровые волонтеры. Диагноз пациентов подтвержден общеклиническими, биохимическими, коагулометрическими и иммуноферментными методами исследования, для оценки экспрессии антигенов использовался многопараметрический цитометрический анализ. Результаты. При анализе экспрессии маркеров показано изменение числа эндотелиальных клеток, мезенхимальных стволовых клеток (МСК) и гемопоэтических стволовых клеток (ГСК) в крови в зависимости от патологии. Эндотелиальные клетки миелоидного (CD45CD14CD34CD309CD144CD31) и немиелоидного (CD45CD14CD34CD309CD144CD31) происхождения, CD309-эндотелиальные клетки и МСК (CD44CD73CD90CD105) предлагаются в качестве маркеров повреждения эндотелия при диабетической симптоматике. При этом ГСК (CD45CD34) могут выступать ценным диагностическим и прогностическим маркером воспаления. Заключение. Для подтверждения сосудистых повреждений и прогноза развития осложнений при диабете 1 и 2 типа в венозной крови пациентов целесообразно оценивать эндотелиальные прогениторные клетки (ЭПК) не костномозговой локализации (CD31CD309CD144) и костномозговой локализации (CD34CD309), и ЭПК c высоким регенеративным потенциалом (CD45CD34CD31CD144). Циркулирующие ЭПК, формирующие колонии in vitro (CD45CD34CD31), рекомендуется использовать в качестве дифференциального маркера состояния регенерации эндотелия при диабете 2 типа. The aim of this study was to identify mesenchymal stem cells (MSC), hematopoietic stem cells (HSC), mature endothelial cells, and endothelial progenitor cells (EPC) in the blood of healthy volunteers, patients with metabolic syndrome, and type 1 and 2 diabetes mellitus as new, informative cellular markers of vascular complications, endothelial regeneration, and inflammation. Methods. The diagnosis was confirmed by general clinical, biochemical, coagulometeric and ELISA studies; multi-parameter cytometric assay was used for evaluation of antigen expression. Results. Changes in the count of MSC, HSC, mature endothelial cells, and endothelial progenitor cells in blood of patients with metabolic syndrome and type 1 and 2 diabetes depended on the type of pathology. We propose using endothelial cells of myeloid (CD45CD14CD34CD309CD144CD31) and non-myeloid origin (CD45CD14CD34CD309CD144CD31), CD309-endothelial cells, and MSCs with the CD44CD73CD90CD105 phenotype as nonspecific markers of endothelial damage in presence of diabetic symptoms. Furthermore, HSCs (CD45CD34) can be used as a valuable diagnostic and prognostic marker of inflammation. Conclusions. It is relevant to evaluate EPCs of non-bone marrow localization (CD31CD309CD144) and bone marrow localization (CD34CD309) and EPCs with a high regenerative potential (CD45CD34CD31CD144) in the blood of patients with type 1 and 2 diabetes to confirm the presence of vascular damage and predict development of complications. Circulating, in vitro colony-forming EPCs (CD45CD34CD31) are recommended as a differential marker for inhibition of endothelial regeneration in type 2 diabetes.

Abstract 3753: Critical Role of Scavenger Receptor-BI Expressing Bone Marrow-Derived Endothelial Progenitor Cells in the Attenuation of Allograft Vasculopathy by Human Apo A-I Transfer

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Yingmei Feng ◽  
Miranda van Eck ◽  
Eline Van Craeyveld ◽  
Frank Jacobs ◽  
Sophie Van Linthout ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Critical Role ◽  
Scavenger Receptor ◽  
Hdl Cholesterol ◽  
Expression Cassette ◽  
No Production ◽  
Endothelial Progenitor ◽  
Allograft Vasculopathy ◽  
Endothelial Regeneration

Background: Accelerated endothelial regeneration mediated by enhanced endothelial progenitor cell (EPC) incorporation may attenuate the development of allograft vasculopathy. Hypothesis: We investigated the hypothesis that modulation of EPC biology and attenuation of allograft vasculopathy by increased HDL cholesterol following human apo A-I (AdA-I) transfer requires scavenger receptor (SR)-BI expression in bone marrow-derived EPCs. Methods: Bone marrow transplantations with SR-BI+/+ or SR-BI−/− bone marrow were performed 4 weeks before gene transfer or saline injection. E1E3E4-deleted vectors containing a hepatocyte-specific human apo A-I expression cassette or containing no expression cassette were injected via the tail vein. Two weeks later, a common carotid artery of a female Balb/c donor mouse was transplanted paratopically into male recipient C57BL/6 mice. To analyse EPC incorporation, sex mismatch bone marrow transplantations were performed in female C57BL/6 mice and incorporated EPCs were quantified by in situ hybridization for the murine Y-chromosome. Results: Following AdA-I transfer, the number of circulating EPCs increased 2.0-fold (p<0.0001) at different time-points in C57BL/6 mice transplanted with SR-BI+/+ bone marrow but was unaltered in mice with SR-BI−/− bone marrow. The effect of HDL on EPC migration in vitro requires signaling via SR-BI and extracellular signal-regulated kinases (ERK) and is dependent on increased NO production in EPCs. Human apo A-I transfer 2 weeks before paratopic artery transplantation reduced intimal area at day 21 3.7-fold (p<0.001) in mice with SR-BI+/+ bone marrow but had no effect in mice with SR-BI−/− bone marrow. The number of CD31 positive endothelial cells lining the lumen and the number of incorporated EPCs was increased 3.0-fold (p<0.001) and 9.7-fold (p<0.001), respectively, in AdA-I treated chimeric SR-BI+/+ mice compared to control mice with SR-BI+/+ bone marrow. Endothelial regeneration and EPC incorporation was not increased after AdA-I transfer in chimeric SR-BI−/−mice. Conclusion: Human apo A-I transfer-mediated endothelial regeneration to prevent allograft vasculopathy is strictly dependent on SR-BI expressing bone marrow-derived EPCs.

Cerebral Endothelial Regeneration Following Brain Injury

1989 ◽  
pp. 1040-1042
Author(s):  
T. Akimura ◽  
T. Orita ◽  
T. Kamiryo ◽  
Y. Furutani ◽  
T. Nishizaki ◽  
...  
Keyword(s):  
Brain Injury ◽  
Endothelial Regeneration
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