Methoprene-Tolerant, A PAS Gene Critical for Juvenile Hormone Signaling in Drosophila Melanogaster

2003 ◽  
pp. 109-132
Author(s):  
Thomas G. Wilson
Keyword(s):  
Drosophila Melanogaster ◽  
Juvenile Hormone ◽  
Hormone Signaling

Juvenile hormone signaling during oogenesis in Drosophila melanogaster

2002 ◽  
Vol 32 (11) ◽  
pp. 1555-1565 ◽  
Author(s):  
E.B. Dubrovsky ◽  
V.A. Dubrovskaya ◽  
E.M. Berger
Keyword(s):  
Drosophila Melanogaster ◽  
Juvenile Hormone ◽  
Hormone Signaling

Juvenile hormone signaling promotes ovulation and maintains egg shape by inducing expression of extracellular matrix genes

2021 ◽  
Vol 118 (39) ◽  
pp. e2104461118
Author(s):  
Wei Luo ◽  
Suning Liu ◽  
Wenqiang Zhang ◽  
Liu Yang ◽  
Jianhua Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Muscle Contraction ◽  
Juvenile Hormone ◽  
Fat Body ◽  
Collagen Iv ◽  
Hormone Signaling ◽  
Gonadotropic Hormone ◽  
Egg Shape ◽  
Insect Reproduction

It is well documented that the juvenile hormone (JH) can function as a gonadotropic hormone that stimulates vitellogenesis by activating the production and uptake of vitellogenin in insects. Here, we describe a phenotype associated with mutations in the Drosophila JH receptor genes, Met and Gce: the accumulation of mature eggs with reduced egg length in the ovary. JH signaling is mainly activated in ovarian muscle cells and induces laminin gene expression in these cells. Meanwhile, JH signaling induces collagen IV gene expression in the adult fat body, from which collagen IV is secreted and deposited onto the ovarian muscles. Laminin locally and collagen IV remotely contribute to the assembly of ovarian muscle extracellular matrix (ECM); moreover, the ECM components are indispensable for ovarian muscle contraction. Furthermore, ovarian muscle contraction externally generates a mechanical force to promote ovulation and maintain egg shape. This work reveals an important mechanism for JH-regulated insect reproduction.

Dietary components modulate yolk protein gene transcription in Drosophila melanogaster

Development ◽  
1988 ◽  
Vol 103 (1) ◽  
pp. 119-128 ◽  
Author(s):  
M. Bownes ◽  
A. Scott ◽  
A. Shirras
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Juvenile Hormone ◽  
Gene Transcription ◽  
Protein Gene ◽  
Yolk Protein ◽  
Complete Medium ◽  
General Effect ◽  
Total Recovery ◽  
Insect Hormones

The three yolk proteins of Drosophila melanogaster begin to be synthesized at eclosion. Transcription of the genes is regulated by the genes tra, tra-2 and dsx and also by the insect hormones, juvenile hormone and 20-hydroxyecdysone. We show that there is yet another level of control which is dependent upon feeding. Females that are starved from eclosion show a basal level of yolk protein gene transcription, which is rapidly increased when a complete diet is supplied. We show that the effect is not due to incorrect development of the fat body and is unlikely to be solely due to a general effect on protein synthesis. Later in development, cessation of feeding leads to selective inhibition of yolk protein synthesis and hence egg production. The effects of starvation can be partially overcome by 20-hydroxyecdysone, juvenile hormone, casein, amino acid mix or sucrose, but only a complete medium or live yeast brings about total recovery. Using yp1-Adh fusions (fusions of the promoter region of yp1 to the structural gene for Adh), the DNA sequence required for this diet-enhanced transcription has been located within an 890 bp fragment upstream of the yp1 gene. The insect hormones do not operate on this same DNA fragment.

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