Wheat Catalase Expressed in Transgenic Rice Plants Can Improve Tolerance Against Low Temperature Injury

2002 ◽  
pp. 277-287
Author(s):  
Takeshi Matsumuraa ◽  
Noriko Tabayashib ◽  
Yasuyo Kamagata ◽  
Chihiro Souma ◽  
Haruo Saruyama
Keyword(s):  
Low Temperature ◽  
Transgenic Rice ◽  
Rice Plants ◽  
Transgenic Rice Plants

scholarly journals Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice

2021 ◽  
Vol 12 ◽  
Author(s):  
Mi Young Byun ◽  
Li Hua Cui ◽  
Andosung Lee ◽  
Hyung Geun Oh ◽  
Yo-Han Yoo ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Low Temperature ◽  
Cold Tolerance ◽  
Transgenic Rice ◽  
Flowering Plant ◽  
Deschampsia Antarctica ◽  
Actin Depolymerizing Factor ◽  
Rice Plants ◽  
Transgenic Rice Plants ◽  
The Antarctic

The Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance that allow D. antarctica to colonize harsh Antarctic environments; however, the molecular mechanisms of its responses to environmental changes remain poorly understood. To elucidate the survival strategies of D. antarctica in Antarctic environments, we investigated the functions of actin depolymerizing factor (ADF) in this species. We identified eight ADF genes in the transcriptome that were clustered into five subgroups by phylogenetic analysis. DaADF3, which belongs to a monocot-specific clade together with cold-responsive ADF in wheat, showed significant transcriptional induction in response to dehydration and cold, as well as under Antarctic field conditions. Multiple drought and low-temperature responsive elements were identified as possible binding sites of C-repeat-binding factors in the promoter region of DaADF3, indicating a close relationship between DaADF3 transcription control and abiotic stress responses. To investigate the functions of DaADF3 related to abiotic stresses in vivo, we generated transgenic rice plants overexpressing DaADF3. These transgenic plants showed greater tolerance to low-temperature stress than the wild-type in terms of survival rate, leaf chlorophyll content, and electrolyte leakage, accompanied by changes in actin filament organization in the root tips. Together, our results imply that DaADF3 played an important role in the enhancement of cold tolerance in transgenic rice plants and in the adaptation of D. antarctica to its extreme environment.

Cloning of New Herbicide Resistant Gene in Soil Metagenomics and Generation of Transgenic Rice Plants

2014 ◽  
Vol 40 (7) ◽  
pp. 1190
Author(s):  
Yun-Peng WANG ◽  
Jing-Yong MA ◽  
Rui MA ◽  
Jian MA ◽  
Wen-Guo LIU
Keyword(s):  
Transgenic Rice ◽  
Herbicide Resistant ◽  
Rice Plants ◽  
Resistant Gene ◽  
Transgenic Rice Plants ◽  
Soil Metagenomics

scholarly journals Production and characterization of transgenic rice plants carrying a high-affinity nitrate transporter gene (OsNRT2.1)

2009 ◽  
Vol 59 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Hisato Katayama ◽  
Mari Mori ◽  
Yoko Kawamura ◽  
Toshinori Tanaka ◽  
Masashi Mori ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Nitrate Transporter ◽  
Transporter Gene ◽  
Rice Plants ◽  
High Affinity ◽  
Transgenic Rice Plants ◽  
Nitrate Transporter Gene

scholarly journals Overexpression of a specific OsGSTL2 isoenzyme improves glyphosate and chlorsulfuron tolerance of transgenic rice plants

2013 ◽  
Vol 8 (16) ◽  
pp. 1520-1527 ◽  
Author(s):  
Hu Tingzhang ◽  
Yang Junnian ◽  
Wu Xiaoli ◽  
Huang Xiaoyun ◽  
Chen Zaigang ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Rice Plants ◽  
Transgenic Rice Plants

scholarly journals miR2105 regulates ABA biosynthesis via OsbZIP86-OsNCED3 module to contribute to drought tolerance in rice

2021 ◽  
Author(s):  
Weiwei Gao ◽  
Mingkang Li ◽  
Songguang Yang ◽  
Chunzhi Gao ◽  
Yan Su ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Transgenic Rice ◽  
Crucial Role ◽  
Agronomic Traits ◽  
Stomatal Closure ◽  
Plant Tolerance ◽  
Rice Plants ◽  
Transgenic Rice Plants ◽  
Aba Biosynthesis

AbstractInduced abscisic acid (ABA) biosynthesis plays an important role in plant tolerance to abiotic stresses, including drought, cold and salinity. However, regulation pathway of the ABA biosynthesis in response to stresses is unclear. Here, we identified a rice miRNA, osa-miR2105 (miR2105), which plays a crucial role in ABA biosynthesis under drought stress. Analysis of expression, transgenic rice and cleavage site showed that OsbZIP86 is a target gene of miR2105. Subcellular localization and luciferase activity assays showed that OsbZIP86 is a nuclear transcription factor. In vivo and in vitro analyses showed that OsbZIP86 directly binds to the promoter of OsNCED3, and interacts with OsSAPK10, resulting in enhanced-expression of OsNCED3. Transgenic rice plants with knock-down of miR2105 or overexpression of OsbZIP86 showed higher ABA content, more tolerance to drought, a lower rate of water loss, more stomatal closure than wild type rice ZH11 under drought stress. These rice plants showed no penalty with respect to agronomic traits under normal conditions. By contrast, transgenic rice plants with miR2105 overexpression, OsbZIP86 downregulation, or OsbZIP86 knockout displayed less tolerance to drought stress and other phenotypes. Collectively, our results show that a regulatory network of ‘miR2105-OsSAPK10/OsbZIP86-OsNCED3’ control ABA biosynthesis in response to drought stress.One-sentence summary‘miR2105-OsbZIP86-OsNCED3’ module plays crucial role in mediating ABA biosynthesis to contribute to drought tolerance with no penalty with respect to agronomic traits under normal conditions.

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