Monoclonal Antibodies to Langerhans Cells in Man and Experimental Animals

1991 ◽  
pp. 25-38 ◽  
Author(s):  
Georg Kraal
Keyword(s):  
Monoclonal Antibodies ◽  
Langerhans Cells ◽  
Experimental Animals

Monoclonal antibodies in cancer therapy.

1983 ◽  
Vol 1 (9) ◽  
pp. 582-590 ◽  
Author(s):  
R K Oldham
Keyword(s):  
Monoclonal Antibody ◽  
Clinical Trials ◽  
Monoclonal Antibodies ◽  
Cancer Therapy ◽  
Experimental Approach ◽  
Preliminary Evidence ◽  
Current Status ◽  
Experimental Animals ◽  
Specific Targeting ◽  
Specific Tumor

The need for improved specificity in cancer therapy is apparent. With the advent of monoclonal antibodies, the possibility of specifically targeted therapy is being considered. Early trials of monoclonal antibody in experimental animals and humans have indicated its ability to traffic to specific tumor sites and to localize on or around the tumor cells displaying antigens to which the antibody is directed. This evidence of specific targeting, along with preliminary evidence of therapeutic efficacy for monoclonal antibodies and immunoconjugates with drugs, toxins, and isotopes is encouraging. The current status of clinical trials with monoclonal antibodies is reviewed and an example of the experimental approach for the development of immunoconjugates in animal models is presented.

scholarly journals Enrichment of Murine and Human Langerhans Cells with Solid Phase Immunoabsorption Using Pan-Leukocyte Monoclonal Antibodies

1985 ◽  
Vol 84 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Gary S. Wood ◽  
Jon Kosek ◽  
Eugene C. Butcher ◽  
Vera B. Morhenn
Keyword(s):  
Monoclonal Antibodies ◽  
Langerhans Cells ◽  
Solid Phase

scholarly journals Heterogeneity of rat macrophages recognized by monoclonal antibodies: an immunohistochemical and immunoelectron microscopic study.

1989 ◽  
Vol 37 (5) ◽  
pp. 635-641 ◽  
Author(s):  
M Takeya ◽  
L Hsiao ◽  
Y Shimokawa ◽  
K Takahashi
Keyword(s):  
Monoclonal Antibodies ◽  
Langerhans Cells ◽  
Peritoneal Macrophages ◽  
Immunoperoxidase Method ◽  
Reaction Products ◽  
Tissue Sections ◽  
Cytoplasmic Vacuoles ◽  
A Cell ◽  
Thymic Medulla ◽  
Splenic Red Pulp

Three monoclonal antibodies, designated RM-1, TRPM-1, and TRPM-2, were raised against rat peritoneal macrophages. By the immunoperoxidase method, antigens recognized by these antibodies were distributed throughout most tissue and free macrophages examined, including those of splenic red pulp, lymphatic sinus, connective tissue, and peritoneal cavity, as well as Kupffer cells of liver and alveolar macrophages. The numbers of positive cells were different for each antibody. RM-1 and TRPM-1 were also reactive with interdigitating cells (IDCs) in the thymus-dependent area and with Langerhans cells in the skin, whereas TRPM-2 failed to demonstrate IDCs in thymic medulla and Langerhans cells. The reactions of each antibody were observed by immunoelectron microscopy in the different ultrastructural compartments of the cells. RM-1 recognized a cell surface antigen; reaction products for TRPM-1 were found on a part of the cell membrane and in the cytoplasmic vacuoles; and those of TRPM-2 were present along the nuclear envelope and intracytoplasmic vacuoles. These antibodies seem to be useful not only for the detection of macrophages in tissue sections but also for investigation of macrophage heterogeneity in different tissues.

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