Observations on Single Calcium Channels

1983 ◽  
pp. 437-449 ◽  
Author(s):  
H. D. Lux
Keyword(s):  
Calcium Channels ◽  
Single Calcium Channels

Single calcium channels in resistance-sized cerebral arteries from rats

1993 ◽  
Vol 264 (2) ◽  
pp. H470-H478 ◽  
Author(s):  
J. M. Quayle ◽  
J. G. McCarron ◽  
J. R. Asbury ◽  
M. T. Nelson
Keyword(s):  
Steady State ◽  
Calcium Channels ◽  
Single Channel ◽  
Cerebral Arteries ◽  
Membrane Depolarization ◽  
Barium Concentration ◽  
Voltage Dependent ◽  
Steady State Inactivation ◽  
Wistar Kyoto ◽  
Single Calcium Channels

Unitary currents through single calcium channels were measured from cell-attached patches on smooth muscle cells isolated from resistance-sized branches of posterior cerebral arteries from Wistar-Kyoto normotensive rats. Barium (80 and 10 mM) was used as the charge carrier, with and without the dihydropyridine calcium channel agonist BAY R 5417. Unitary currents decreased on membrane depolarization, with a slope conductance of 19.4 pS (80 mM barium). Channel open-state probability (Po) was steeply voltage dependent. Peak Po during test pulses from -70 mV increased e-fold per 4.5-mV depolarization. Mean peak Po at potentials positive to +10 mV was 0.44. Po at steady membrane potentials was also steeply voltage dependent, changing e-fold per 4.5 mV in the absence of inactivation. Steady-state Po at positive potentials was substantially lower than peak Po elicited by test pulses, suggesting that steady-state inactivation can reduce Po by as much as 10-fold. Membrane depolarization decreased the longest mean closed time but had little effect on the mean open time of single calcium channels measured during steady-state recordings. Lowering the external barium concentration from 80 to 10 mM reduced the single channel conductance to 12.4 pS and shifted the relationship between steady-state Po and membrane potential by about -30 mV. BAY R 5417 also shifted this relationship by about -15 mV.

Single calcium channels in neurons of rat spinal ganglia

1986 ◽  
Vol 17 (5) ◽  
pp. 490-497
Author(s):  
Ya. M. Shuba ◽  
A. N. Savchenko
Keyword(s):  
Calcium Channels ◽  
Spinal Ganglia ◽  
Single Calcium Channels

scholarly journals Maitotoxin-activated single calcium channels in guinea-pig cardiac cells

1987 ◽  
Vol 92 (3) ◽  
pp. 665-671 ◽  
Author(s):  
Masaki Kobayashi ◽  
Rikuo Ochi ◽  
Yasushi Ohizumi
Keyword(s):  
Guinea Pig ◽  
Calcium Channels ◽  
Cardiac Cells ◽  
Single Calcium Channels

Properties of single calcium channels in cardiac cell culture

Nature ◽  
1982 ◽  
Vol 297 (5866) ◽  
pp. 501-504 ◽  
Author(s):  
H. Reuter ◽  
C. F. Stevens ◽  
R. W. Tsien ◽  
G. Yellen
Keyword(s):  
Cell Culture ◽  
Calcium Channels ◽  
Cardiac Cell ◽  
Single Calcium Channels
Sign in / Sign up

Export Citation Format

Share Document