In Situ NADH Laser Fluorimetry and Its Application to the Study of Cardiac Metabolism

1985 ◽  
pp. 229-238 ◽  
Author(s):  
Guy Renault ◽  
Elisabeth Raynal ◽  
Martine Sinet ◽  
Martine Muffat-Joly ◽  
Jean Cornillault ◽  
...  
Keyword(s):  
Cardiac Metabolism ◽  
Laser Fluorimetry

In situ NADH laser fluorimetry during muscle contraction in humans

1991 ◽  
Vol 63 (1) ◽  
pp. 36-42 ◽  
Author(s):  
C. Y. Guezennec ◽  
F. Lienhard ◽  
F. Louisy ◽  
G. Renault ◽  
M. H. Tusseau ◽  
...  
Keyword(s):  
Muscle Contraction ◽  
Laser Fluorimetry

In Situ Detection Of Tissular Respiration Arrest By NADH Laser Fluorimetry

1987 ◽  
Author(s):  
Guy Renault ◽  
Martine Muffat-Joly ◽  
Juliette Polianski ◽  
Roger I. Hardy ◽  
Remy Nottin ◽  
...  
Keyword(s):  
Laser Fluorimetry ◽  
In Situ Detection

In situ double-beam NADH laser fluorimetry: choice of a reference wavelength

1984 ◽  
Vol 246 (4) ◽  
pp. H491-H499 ◽  
Author(s):  
G. Renault ◽  
E. Raynal ◽  
M. Sinet ◽  
M. Muffat-Joly ◽  
J. P. Berthier ◽  
...  
Keyword(s):  
Blood Circulation ◽  
Mathematical Relationship ◽  
Perfused Rat Heart ◽  
Laser Fluorimetry ◽  
Beam Laser ◽  
Double Beam ◽  
On Line ◽  
Laser Fluorimeter ◽  
Living Tissues

A double-beam laser fluorimeter, using a single optical fiber to guide the lights, was constructed for in situ and on-line monitoring of NADH concentration [( NADH]) from normally blood-perfused living tissues. The device was tested on an isolated blood-perfused rat heart system to determine the most efficient reference wavelength for the compensation of the hemodynamic artifact induced by blood circulation in the tissues on the fluorescence measure; 586 nm was found to be an accurate reference wavelength, and a mathematical relationship was established that allowed the digital treatment of the measured fluorescence to give a signal (compensated fluorescence) that varied only with [NADH] in the volume of tissue investigated.

NADH in situ laser fluorimetry: Effect of pentobarbital on continuously monitored myocardial redox state

1987 ◽  
Vol 7 (4) ◽  
pp. 339-346 ◽  
Author(s):  
Guy Renault ◽  
Martine Muffat-Joly ◽  
Juliette Polianski ◽  
Roger Ian Hardy ◽  
Jean-Louis Boutineau ◽  
...  
Keyword(s):  
Redox State ◽  
Laser Fluorimetry

In situ NADH laser fluorimetry of rat fast- and slow-twitch muscles during tetanus

1988 ◽  
Vol 64 (6) ◽  
pp. 2692-2695 ◽  
Author(s):  
D. Duboc ◽  
M. Muffat-Joly ◽  
G. Renault ◽  
M. Degeorges ◽  
M. Toussaint ◽  
...  
Keyword(s):  
Nondestructive Method ◽  
Base Line ◽  
Sprague Dawley ◽  
Continuous Increase ◽  
Laser Fluorimetry ◽  
Oxidation Reduction ◽  
Sprague Dawley Rats ◽  
Rate Of Recovery ◽  
Slow Twitch

To investigate the variations of oxidation-reduction status of fast- and slow-twitch muscles during intense contractions, we performed in situ NADH laser fluorimetry during 25-s tetanus in extensor digitorum longus (EDL) and in soleus (SOL) muscles of eight Sprague-Dawley rats anesthetized with pentobarbital sodium. At base line the compensated NADH fluorescence (F0) was not significantly different between EDL and SOL. In EDL, tetanic stimulation induced an increase of F0, which rapidly reached a plateau that was 124% over the base-line value and stable until the end of the stimulation. In SOL, after an initial shouldering there was a continuous increase of F0 until the end of tetanus, reaching 275% of the base-line value. After the stimulation the initial rate of recovery was significantly faster in SOL than in EDL. We conclude that during and after intense contraction the variation of NADH content vs. time can be evaluated by in situ NADH laser fluorimetry in different muscle types. This nondestructive method can be helpful to differentiate in situ the various physiological or pathological oxidative capabilities of skeletal muscles.

In situ monitoring of myocardial metabolism by laser fluorimetry: Relevance of a test of local ischemia

1985 ◽  
Vol 5 (2) ◽  
pp. 111-122 ◽  
Author(s):  
Guy Renault ◽  
Martine Sinet ◽  
Martine Muffat-Joly ◽  
Jean Cornillault ◽  
Jean Jacques Pocidalo
Keyword(s):  
Myocardial Metabolism ◽  
In Situ Monitoring ◽  
Local Ischemia ◽  
Laser Fluorimetry

Rendez vous with Saturn's rings

1984 ◽  
Vol 75 ◽  
pp. 743-759 ◽  
Author(s):  
Kerry T. Nock
Keyword(s):  
Solar Energy ◽  
Electric Propulsion ◽  
Power Source ◽  
Propulsion System ◽  
Low Thrust ◽  
Ring Plane ◽  
The Earth ◽  
Total Impulse ◽  
Saturn's Rings

ABSTRACTA mission to rendezvous with the rings of Saturn is studied with regard to science rationale and instrumentation and engineering feasibility and design. Future detailedin situexploration of the rings of Saturn will require spacecraft systems with enormous propulsive capability. NASA is currently studying the critical technologies for just such a system, called Nuclear Electric Propulsion (NEP). Electric propulsion is the only technology which can effectively provide the required total impulse for this demanding mission. Furthermore, the power source must be nuclear because the solar energy reaching Saturn is only 1% of that at the Earth. An important aspect of this mission is the ability of the low thrust propulsion system to continuously boost the spacecraft above the ring plane as it spirals in toward Saturn, thus enabling scientific measurements of ring particles from only a few kilometers.

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