Autoimmunity to Myelin Basic Protein

1982 ◽  
pp. 3-22 ◽  
Author(s):  
Robert H. Swanborg ◽  
James H. Holda ◽  
Joyce A. Killen
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein

scholarly journals Myelin basic protein enhances axonal regeneration from neural progenitor cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhengjian Yan ◽  
Lei Chu ◽  
Xiaojiong Jia ◽  
Lu Lin ◽  
Si Cheng
Keyword(s):  
Myelin Basic Protein ◽  
Neurite Outgrowth ◽  
Progenitor Cells ◽  
Axonal Regeneration ◽  
Neural Progenitor Cells ◽  
Basic Protein ◽  
Neural Progenitor ◽  
Dependent Manner ◽  
Cord Injury

Abstract Introduction Stem cell therapy using neural progenitor cells (NPCs) shows promise in mitigating the debilitating effects of spinal cord injury (SCI). Notably, myelin stimulates axonal regeneration from mammalian NPCs. This led us to hypothesize that myelin-associated proteins may contribute to axonal regeneration from NPCs. Methods We conducted an R-based bioinformatics analysis to identify key gene(s) that may participate in myelin-associated axonal regeneration from murine NPCs, which identified the serine protease myelin basic protein (Mbp). We employed E12 murine NPCs, E14 rat NPCs, and human iPSC-derived Day 1 NPCs (D1 hNPCs) with or without CRISPR/Cas9-mediated Mbp knockout in combination with rescue L1-70 overexpression, constitutively-active VP16-PPARγ2, or the PPARγ agonist ciglitazone. A murine dorsal column crush model of SCI utilizing porous collagen-based scaffolding (PCS)-seeded murine NPCs with or without stable Mbp overexpression was used to assess locomotive recovery and axonal regeneration in vivo. Results Myelin promotes axonal outgrowth from NPCs in an Mbp-dependent manner and that Mbp’s stimulatory effects on NPC neurite outgrowth are mediated by Mbp’s production of L1-70. Furthermore, we determined that Mbp/L1-70’s stimulatory effects on NPC neurite outgrowth are mediated by PPARγ-based repression of neuron differentiation-associated gene expression and PPARγ-based Erk1/2 activation. In vivo, PCS-seeded murine NPCs stably overexpressing Mbp significantly enhanced locomotive recovery and axonal regeneration in post-SCI mice. Conclusions We discovered that Mbp supports axonal regeneration from mammalian NPCs through the novel Mbp/L1cam/Pparγ signaling pathway. This study suggests that bioengineered, NPC-based interventions can promote axonal regeneration and functional recovery post-SCI.

scholarly journals Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein.

1983 ◽  
Vol 258 (2) ◽  
pp. 930-937 ◽  
Author(s):  
R E Martenson ◽  
M J Law ◽  
G E Deibler
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Phosphorylation Sites

scholarly journals The N terminus of human myelin basic protein consists of C2, C4, C6, and C8 alkyl carboxylic acids.

1992 ◽  
Vol 267 (14) ◽  
pp. 9779-9782
Author(s):  
M.A. Moscarello ◽  
H Pang ◽  
C.R. Pace-Asciak ◽  
D.D. Wood
Keyword(s):  
Myelin Basic Protein ◽  
Carboxylic Acids ◽  
Basic Protein ◽  
N Terminus

scholarly journals Chromatographic Fractionation of Myelin Basic Protein

1973 ◽  
Vol 248 (7) ◽  
pp. 2392-2396
Author(s):  
Gladys E. Deibler ◽  
Russell E. Martenson
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Chromatographic Fractionation
Sign in / Sign up

Export Citation Format

Share Document