Renal Brush Border Membranes in X-Linked Hypophosphatemic Mice: A Study of the Protein Kinase Activity

1984 ◽  
pp. 37-40
Author(s):  
Michèle G. Brunette ◽  
Richard Béliveau
Keyword(s):  
Protein Kinase ◽  
Brush Border ◽  
Kinase Activity ◽  
Protein Kinase Activity ◽  
Brush Border Membranes ◽  
Renal Brush Border Membranes ◽  
Renal Brush Border

Phosphorylation of type II cAMP-dependent protein kinase in renal brush border membranes

1986 ◽  
Vol 250 (4) ◽  
pp. F659-F666
Author(s):  
M. R. Hammerman
Keyword(s):  
Protein Kinase ◽  
Brush Border ◽  
Regulatory Subunit ◽  
Type Ii ◽  
Dependent Protein Kinase ◽  
Brush Border Membranes ◽  
Camp Dependent Protein Kinase ◽  
Renal Brush Border Membranes ◽  
Dependent Protein ◽  
Renal Brush Border

We have previously demonstrated cAMP-dependent 32P phosphorylation and dephosphorylation of a 62,000 relative molecular weight (Mr) protein in autoradiograms of sodium dodecyl sulfate polyacrylamide gels originating from canine renal brush border membranes. In the current studies 32P phosphorylation of the 62,000 Mr protein that was independent of cAMP was noted in the presence of Zn2+. Under these conditions, cAMP inhibited the 32P phosphorylation of this protein. Concentration-dependent photoaffinity labeling of a band with Mr 60,000 in autoradiograms of gels resulted from incubation of membranes with cyclic 8-azidoadenosine-3',5'-monophosphate (8-N3-[32P]cAMP) followed by exposure to light. In the presence of Zn2+ and ATP, an apparent shift in the Mr of a portion of the photoaffinity-labeled band to 62,000 was seen. The 62,000 Mr phosphoprotein in detergent-solubilized supernatants of brush border membranes was immunoprecipitated with antibodies directed against the regulatory subunit of type II cAMP-dependent protein kinase. Our observations strongly suggest that the 62,000 Mr protein is the regulatory subunit.

scholarly journals PHOSPHATE (Pi) TRANSPORT IN RENAL BRUSH BORDER MEMBRANES (BBMV) IN NEWBORN PUPPIES (P)

1984 ◽  
Vol 18 ◽  
pp. 366A-366A
Author(s):  
Eddie S Moore ◽  
Eunice G John ◽  
Lawrence Rufr ◽  
Christine S Mooers ◽  
Nochik Park ◽  
...  
Keyword(s):  
Brush Border ◽  
Brush Border Membranes ◽  
Pi Transport ◽  
Renal Brush Border Membranes ◽  
Renal Brush Border

Ion pathways in renal brush border membranes

1982 ◽  
Vol 685 (3) ◽  
pp. 260-272 ◽  
Author(s):  
C. Burnham ◽  
C. Munzesheimer ◽  
E. Rabon ◽  
G. Sachs
Keyword(s):  
Brush Border ◽  
Brush Border Membranes ◽  
Renal Brush Border Membranes ◽  
Renal Brush Border

scholarly journals PHOSPHATE (Pi) UPTAKE IN FETAL LAMB (FL) RENAL BRUSH BORDER MEMBRANES (BBMV)

1984 ◽  
Vol 18 ◽  
pp. 366A-366A
Author(s):  
Lawrence Rufer ◽  
Eddie S Moore ◽  
Christine S Mooers ◽  
Nochik Park
Keyword(s):  
Brush Border ◽  
Brush Border Membranes ◽  
Fetal Lamb ◽  
Renal Brush Border Membranes ◽  
Renal Brush Border
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