Lipolytic Activities Operative at the Outer Surface of Rat Fat Cells

1986 ◽  
pp. 35-37
Author(s):  
Alain Vérine ◽  
Paul Salers ◽  
Jean Boyer
Diabetes ◽  
1991 ◽  
Vol 40 (11) ◽  
pp. 1440-1448 ◽  
Author(s):  
H. K. Muller ◽  
M. Kellerer ◽  
B. Ermel ◽  
A. Muhlhofer ◽  
B. Obermaier-Kusser ◽  
...  

1979 ◽  
Vol 254 (18) ◽  
pp. 8903-8908
Author(s):  
R.J. Cabelli ◽  
C.C. Malbon

Life Sciences ◽  
1986 ◽  
Vol 39 (22) ◽  
pp. 2111-2119 ◽  
Author(s):  
Susan K. Fried ◽  
Mario DiGirolamo

1976 ◽  
Vol 154 (1) ◽  
pp. 11-21 ◽  
Author(s):  
J P Luzio ◽  
A C Newby ◽  
C N Hales

1. A rapid method for the isolation of hormonally sensitive rat fat-cell plasma membranes was developed by using immunological techniques. 2. Rabbit anti-(rat erythrocyte) sera were raised and shown to cross-react with isolated rat fat-cells. 3. Isolated rat fat-cells were coated with rabbit anti-(rat erythrocyte) antibodies, homogenized and the homogenate made to react with an immunoadsorbent prepared by covalently coupling donkey anti-(rabbit globulin) antibodies to aminocellulose. Uptake of plasma membrane on to the immunoadsorbent was monitored by assaying the enzymes adenylate cyclase and 5′-nucleotidase and an immunological marker consisting of a 125I-labelled anti-(immunoglobulin G)-anti-cell antibody complex bound to the cells before fractionation. Contamination of the plasma-membrane preparation by other subcellular fractions was also investigated. 4. By using this technique, a method was developed allowing 25-40% recovery of plasma membrane from fat-cell homogenates within 30 min of homogenization. 5. Adenylate cyclase in the isolated plasma-membrane preparation was stimulated by 5 μm-adrenaline.


FEBS Letters ◽  
1987 ◽  
Vol 224 (1) ◽  
pp. 137-141 ◽  
Author(s):  
Yoshihiko Kuroda ◽  
Hidetaka Nakayama ◽  
Teruo Ishibashi ◽  
Shin Aoki ◽  
Satoshi Tushima ◽  
...  
Keyword(s):  

1988 ◽  
Vol 20 (05) ◽  
pp. 257-260 ◽  
Author(s):  
T. Suzuki ◽  
H. Makino ◽  
A. Kanatsuka ◽  
S. Yoshida

1988 ◽  
Vol 150 (1) ◽  
pp. 197-203 ◽  
Author(s):  
J.C. Lawrence ◽  
Jeffrey Hiken ◽  
Barry Burnette ◽  
A.A. DePaoli-Roach

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