Visible Light Induced Killing and Mutation of Acriflavine Sensitized Chinese Hamster Cells

1986 ◽  
pp. 381-387
Author(s):  
Tapan Ganguly ◽  
Sukhendu B. Bhattacharjee
Keyword(s):  
Visible Light ◽  
Chinese Hamster ◽  
Chinese Hamster Cells

INDUCTION OF DNA-PROTEIN CROSS-LINKS IN CHINESE HAMSTER CELLS BY THE PHOTODYNAMIC ACTION OF CHLOROALUMINUM PHTHALOCYANINE AND VISIBLE LIGHT

1988 ◽  
Vol 48 (3) ◽  
pp. 297-303 ◽  
Author(s):  
Narayani Ramakrishnan ◽  
Marian E. Clay ◽  
Liang-YAN Xue ◽  
Helen H. Evans ◽  
Antonio Rodriguez-Antunez ◽  
...  
Keyword(s):  
Visible Light ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Photodynamic Action ◽  
Cross Links ◽  
Chloroaluminum Phthalocyanine

Scanning luminescence x-ray microscopy: Progress toward selective staining using microspheres

1994 ◽  
Vol 52 ◽  
pp. 74-75
Author(s):  
C. Jacobsen ◽  
J. Fu ◽  
S. Mayer ◽  
Y. Wang ◽  
S. Williams
Keyword(s):  
Visible Light ◽  
Active Sites ◽  
Light Emission ◽  
Chinese Hamster ◽  
Polystyrene Spheres ◽  
Good Resistance ◽  
X Ray ◽  
Selective Staining ◽  
Visible Light Emission ◽  
Specific Labelling

In scanning luminescence x-ray microscopy (SLXM), a high resolution x-ray probe is used to excite visible light emission (see Figs. 1 and 2). The technique has been developed with a goal of localizing dye-tagged biochemically active sites and structures at 50 nm resolution in thick, hydrated biological specimens. Following our initial efforts, Moronne et al. have begun to develop probes based on biotinylated terbium; we report here our progress towards using microspheres for tagging.Our initial experiments with microspheres were based on commercially-available carboxyl latex spheres which emitted ~ 5 visible light photons per x-ray absorbed, and which showed good resistance to bleaching under x-ray irradiation. Other work (such as that by Guo et al.) has shown that such spheres can be used for a variety of specific labelling applications. Our first efforts have been aimed at labelling ƒ actin in Chinese hamster ovarian (CHO) cells. By using a detergent/fixative protocol to load spheres into cells with permeabilized membranes and preserved morphology, we have succeeded in using commercial dye-loaded, spreptavidin-coated 0.03μm polystyrene spheres linked to biotin phalloidon to label f actin (see Fig. 3).

Multiplex PCR Analysis of Ultraviolet A and B Induced Delayed and Early Mutations in V79 Chinese Hamster Cells

2004 ◽  
Author(s):  
Jostein Dahle ◽  
Paul Noordhuis ◽  
Trond Stokke ◽  
Debbie Hege Svendsrud ◽  
Egil Kvam
Keyword(s):  
Multiplex Pcr ◽  
Chinese Hamster ◽  
Pcr Analysis ◽  
Ultraviolet A ◽  
Chinese Hamster Cells

scholarly journals Differential activation of the hprt gene on the inactive X chromosome in primary and transformed Chinese hamster cells.

1989 ◽  
Vol 9 (4) ◽  
pp. 1635-1641 ◽  
Author(s):  
S G Grant ◽  
R G Worton
Keyword(s):  
Cell Lines ◽  
X Chromosome ◽  
Gene Activation ◽  
Chinese Hamster ◽  
Fibroblast Cell ◽  
Dna Demethylation ◽  
Hprt Gene ◽  
Primary Fibroblast ◽  
Chinese Hamster Cells ◽  
Inactive X Chromosome

We have investigated the genetic activation of the hprt (hypoxanthine-guanine phosphoribosyltransferase) gene located on the inactive X chromosome in primary and transformed female diploid Chinese hamster cells after treatment with the DNA methylation inhibitor 5-azacytidine (5azaCR). Mutants deficient in HPRT were first selected by growth in 6-thioguanine from two primary fibroblast cell lines and from transformed lines derived from them. These HPRT- mutants were then treated with 5azaCR and plated in HAT (hypoxanthine-methotrexate-thymidine) medium to select for cells that had reexpressed the hprt gene on the inactive X chromosome. Contrary to previous results with primary human cells, 5azaCR was effective in activating the hprt gene in primary Chinese hamster fibroblasts at a low but reproducible frequency of 2 x 10(-6) to 7 x 10(-6). In comparison, the frequency in independently derived transformed lines varied from 1 x 10(-5) to 5 x 10(-3), consistently higher than in the nontransformed cells. This increase remained significant when the difference in growth rates between the primary and transformed lines was taken into account. Treatment with 5azaCR was also found to induce transformation in the primary cell lines but at a low frequency of 4 x 10(-7) to 8 x 10(-7), inconsistent with a two-step model of transformation followed by gene activation to explain the derepression of hprt in primary cells. Thus, these results indicate that upon transformation, the hprt gene on the inactive Chinese hamster X chromosome is rendered more susceptible to action by 5azaCR, consistent with a generalized DNA demethylation associated with the transformation event or with an increase in the instability of an underlying primary mechanism of X inactivation.

The Cytotoxicity of 27 Chemicals to V79 Chinese Hamster Cells

1987 ◽  
Vol 15 (1) ◽  
pp. 30-32
Author(s):  
Michael Garle ◽  
Alison H. Hammond ◽  
Jeffrey R. Fry
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cells

Cytotoxic and mutagenic effects of sterigmatocystin on cultured Chinese hamster cells

1981 ◽  
Vol 2 (10) ◽  
pp. 945-949 ◽  
Author(s):  
Kouichi Noda ◽  
Makoto Umeda ◽  
Yoshio Ueno
Keyword(s):  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Mutagenic Effects

scholarly journals Modification of the repair of potentially lethal damage in plateau-phase Chinese hamster cells by 2-chlorodeoxyadenosine.

1988 ◽  
Vol 29 (3) ◽  
pp. 172-181 ◽  
Author(s):  
KIYOSHI TANABE ◽  
WAKAKO HIRAOKA ◽  
MIKINORI KUWABARA ◽  
AKIRA MATSUDA ◽  
TOHRU UEDA ◽  
...  
Keyword(s):  
Chinese Hamster ◽  
Plateau Phase ◽  
Chinese Hamster Cells ◽  
Lethal Damage

Polyploidy Induced by X-Rays during the Cell Cycle of Chinese Hamster Cells in Vitro

1972 ◽  
Vol 52 (3) ◽  
pp. 509 ◽  
Author(s):  
C. K. Yu ◽  
W. K. Sinclair
Keyword(s):  
Cell Cycle ◽  
Chinese Hamster ◽  
X Rays ◽  
Chinese Hamster Cells
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