Sequencing an Unknown Peptide

1991 ◽  
pp. 135-138 ◽  
Author(s):  
N. Poppe-Schriemer ◽  
D. R. Binding ◽  
W. Ens ◽  
J. C. Martens ◽  
F. Mayer ◽  
...  
Keyword(s):  
Unknown Peptide

Unknown Peptide Sequencing Using Matrix-Assisted Laser Desorption/Ionization and In-Source Decay

1998 ◽  
Vol 70 (6) ◽  
pp. 1214-1222 ◽  
Author(s):  
Duane C. Reiber ◽  
Robert S. Brown ◽  
Scot Weinberger ◽  
James Kenny ◽  
Jerome Bailey
Keyword(s):  
Laser Desorption ◽  
Peptide Sequencing ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Unknown Peptide ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Detection oftrans–cisflips and peptide-plane flips in protein structures

2015 ◽  
Vol 71 (8) ◽  
pp. 1604-1614 ◽  
Author(s):  
Wouter G. Touw ◽  
Robbie P. Joosten ◽  
Gert Vriend
Keyword(s):  
Structure Function ◽  
Web Service ◽  
Protein Data Bank ◽  
Protein Structures ◽  
Data Bank ◽  
Peptide Bond ◽  
Peptide Bonds ◽  
Unknown Peptide ◽  
Peptide Plane ◽  
Service Interface

A coordinate-based method is presented to detect peptide bonds that need correction either by a peptide-plane flip or by atrans–cisinversion of the peptide bond. When applied to the whole Protein Data Bank, the method predicts 4617trans–cisflips and many thousands of hitherto unknown peptide-plane flips. A few examples are highlighted for which a correction of the peptide-plane geometry leads to a correction of the understanding of the structure–function relation. All data, including 1088 manually validated cases, are freely available and the method is available from a web server, a web-service interface and throughWHAT_CHECK.

scholarly journals Leaderless secreted peptide signaling molecule alters global gene expression and increases virulence of a human bacterial pathogen

2017 ◽  
Vol 114 (40) ◽  
pp. E8498-E8507 ◽  
Author(s):  
Hackwon Do ◽  
Nishanth Makthal ◽  
Arica R. VanderWal ◽  
Mandy Rettel ◽  
Mikhail M. Savitski ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Bacterial Pathogen ◽  
Global Gene Expression ◽  
Intercellular Signaling ◽  
Human Pathogen ◽  
Unknown Peptide ◽  
Peptide Secretion ◽  
Complex Signaling

Successful pathogens use complex signaling mechanisms to monitor their environment and reprogram global gene expression during specific stages of infection. Group A Streptococcus (GAS) is a major human pathogen that causes significant disease burden worldwide. A secreted cysteine protease known as streptococcal pyrogenic exotoxin B (SpeB) is a key virulence factor that is produced abundantly during infection and is critical for GAS pathogenesis. Although identified nearly a century ago, the molecular basis for growth phase control of speB gene expression remains unknown. We have discovered that GAS uses a previously unknown peptide-mediated intercellular signaling system to control SpeB production, alter global gene expression, and enhance virulence. GAS produces an eight-amino acid leaderless peptide [SpeB-inducing peptide (SIP)] during high cell density and uses the secreted peptide for cell-to-cell signaling to induce population-wide speB expression. The SIP signaling pathway includes peptide secretion, reimportation into the cytosol, and interaction with the intracellular global gene regulator Regulator of Protease B (RopB), resulting in SIP-dependent modulation of DNA binding and regulatory activity of RopB. Notably, SIP signaling causes differential expression of ∼14% of GAS core genes. Several genes that encode toxins and other virulence genes that enhance pathogen dissemination and infection are significantly up-regulated. Using three mouse infection models, we show that the SIP signaling pathway is active during infection and contributes significantly to GAS pathogenesis at multiple host anatomic sites. Together, our results delineate the molecular mechanisms involved in a previously undescribed virulence regulatory pathway of an important human pathogen and suggest new therapeutic strategies.

Sequencing an “unknown” peptide by time-of-flight secondary ion mass spectrometry

1991 ◽  
Vol 111 ◽  
pp. 301-315 ◽  
Author(s):  
N. Poppe-Schriemer ◽  
D.R. Binding ◽  
W. Ens ◽  
F. Mayer ◽  
K.G. Standing ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Ion Mass Spectrometry ◽  
Unknown Peptide ◽  
Secondary Ion
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