Characterization of Liposomal Systems Entrapping Boron-Containing Compounds in Response to pH Gradients

1996 ◽  
pp. 259-275 ◽  
Author(s):  
Angela M. Moraes ◽  
Maria Helena A. Santana ◽  
Ruben G. Carbonell
Keyword(s):  
Ph Gradients

Chromatofocusing and isoelectric focusing in immobilized pH gradients compared for characterization of human hemoglobin variants.

1989 ◽  
Vol 35 (3) ◽  
pp. 425-430 ◽  
Author(s):  
R Paleari ◽  
C Arcelloni ◽  
R Paroni ◽  
I Fermo ◽  
A Mosca
Keyword(s):  
Los Angeles ◽  
Isoelectric Focusing ◽  
Ph Gradient ◽  
Resolving Power ◽  
Human Hemoglobin ◽  
Ph Gradients ◽  
Hemoglobin Variants

Abstract We compared the performance of two highly resolving methods, chromatofocusing (CRF) and isoelectric focusing in immobilized pH gradients (IPGF), for the separation of human hemoglobin variants. Lysates containing 13 different hemoglobins, including variants of clinical and geographical importance, and four electrophoretically "silent" variants (Hb Brockton, Hb Cheverly, Hb Köln, and Hb Waco) were analyzed. Both techniques showed a good intrarun precision (CV = 0.87% for CRF, 0.27% for IPGF) and high and similar resolving power (0.010 pH units, with the pH gradients used in this work). The use of an ultranarrow IPGF range (pH 7.15-7.35; pH gradient = 0.019 pH/cm) allowed the resolution between Hb Brockton, Hb Köln, and Hb A. In some cases (Hb D-Los Angeles, Hb F, Hb Waco), the variants were separated from Hb A in different orders, depending on which technique was used, probably because of the different analytical principles of the two methods. As a second-level test, both procedures are informative for characterization of human hemoglobin variants.

Characterization of Antibody Charge Heterogeneity Resolved by Preparative Immobilized pH Gradients

2010 ◽  
Vol 82 (9) ◽  
pp. 3510-3518 ◽  
Author(s):  
Charlie D. Meert ◽  
Lowell J. Brady ◽  
Amy Guo ◽  
Alain Balland
Keyword(s):  
Charge Heterogeneity ◽  
Ph Gradients

scholarly journals Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. II. Buffering Capacity and Conductance of Isoionic Ampholytes. A Correction.

1971 ◽  
Vol 25 ◽  
pp. 2768-2769 ◽  
Author(s):  
Harry Rilbe ◽  
Kjell Haugsten ◽  
Svend Erik Rasmussen ◽  
Sigfrid Svensson ◽  
J. Koskikallio ◽  
...  
Keyword(s):  
Buffering Capacity ◽  
Ph Gradients ◽  
Fractionation Analysis

Characterization of liposomal systems containing doxorubicin entrapped in response to pH gradients

1990 ◽  
Vol 1025 (2) ◽  
pp. 143-151 ◽  
Author(s):  
Lawrence D. Mayer ◽  
Linda C.L. Tai ◽  
Marcel B. Bally ◽  
George N. Mitilenes ◽  
Richard S. Ginsberg ◽  
...  
Keyword(s):  
Ph Gradients

Isoelectric focusing in immobilized pH gradients with carrier ampholytes added for high-resolution phenotyping of bovine β-lactoglobulins: Characterization of a new genetic variant

1988 ◽  
Vol 9 (9) ◽  
pp. 609-613 ◽  
Author(s):  
Ingolf Krause ◽  
Johann Buchberger ◽  
Günther Weiß ◽  
Monika Pflügler ◽  
Henning Klostermeyer
Keyword(s):  
High Resolution ◽  
Isoelectric Focusing ◽  
Genetic Variant ◽  
Ph Gradients ◽  
Carrier Ampholytes

Characterization of proteins by sequential isoelectric focusing on immobilized pH gradients and electrospray mass spectrometry

1995 ◽  
Vol 16 (1) ◽  
pp. 1381-1384 ◽  
Author(s):  
Umberto Breme ◽  
Jer??me Breton ◽  
Carlo Visco ◽  
Gaetano Orsini ◽  
Pier Giorgio Righetti
Keyword(s):  
Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Electrospray Mass Spectrometry ◽  
Ph Gradients
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