Clonal Propagation of Chilopsis Linearis (CAV.) Through Tissue Culture

1985 ◽  
pp. 354-355
Author(s):  
David W. Still ◽  
John F. Hubstenberger ◽  
Gregory C. Phillips ◽  
Ronald F. Hooks
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Chilopsis Linearis

Rapid Clonal Propagation of Chinese Medicinal Herbs by Tissue Culture

1993 ◽  
pp. 300-304 ◽  
Author(s):  
T. G. Gau ◽  
D. D. Chang ◽  
Y. C. Chern ◽  
C. C. Chen ◽  
F. T. Yeh ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Medicinal Herbs ◽  
Chinese Medicinal Herbs

scholarly journals Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. LUKMAN ◽  
. AGUS-PURWITO
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Randomized Design ◽  
Top Soil ◽  
Completely Randomized Design ◽  
Reduced Light ◽  
In Vitro Micrografting ◽  
Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..

scholarly journals The Various Methods used for the Clonal Propagation in Horticultural Crops: A Review

2021 ◽  
Author(s):  
Kunal Sharma ◽  
Tanishka Thapa
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
The Other ◽  
Asexual Propagation ◽  
Horticultural Crops ◽  
Different Types ◽  
Yield Quality ◽  
Propagation Methods ◽  
The Individual ◽  
Individual Parent

The propagation of plant is required for the purpose of the multiplication of new plants. The producers want desired character of plant and for that purpose they need true to type plants. The true to type plants can’t be produce by the sexual reproduction. The different types of clonal propagation methods can be used for the producing true to type plants Clone means plants which are the exact copy of the individual parents by asexual propagation. Clonal propagation can be used as a method of the production of the genetically identical copies of the individual parent. The clones are required for the purpose of producing identical plants as sexually propagated plants have different variations which can affect the yield, quality and the other characters. To avoid these variations asexual methods are promoted for the propagation of genetically identical new plant such as cutting, layering, tissue culture etc. Many researchers have carried out the work to develop new and different techniques for the sole purpose of clonal propagation of plant.

scholarly journals Clonal propagation of galanga [Languas galanga (L.) Stuntz] through tissue culture

1969 ◽  
Vol 78 (3-4) ◽  
pp. 147-152
Author(s):  
Feiko H. Ferwerda
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Axillary Shoots ◽  
Rate Of Increase ◽  
Repeated Subculture

Buds of rhizomes from greenhouse grown galanga plants were excised and propagated clonally through tissue culture. The medium consisted of Murashige and Skoog (MS) salts, vitamins, 2% sucrose and 2 mg/L 6-benzylaminopurine (BA). Adventitious and axillary shoots were produced by repeated subculture at 4-week intervals. The rate of increase per explant was calculated conservatively to be four plants per plant per 4-week cycle.

Clonal propagation of Cinnamomum zeylanicum Breyn. by tissue culture

1987 ◽  
Vol 9 (1) ◽  
pp. 81-88 ◽  
Author(s):  
V. Ravi Shankar Rai ◽  
K. S. Jagadish Chandra
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Cinnamomum Zeylanicum

Clonal propagation of Zizyphus species through tissue culture

1992 ◽  
Vol 51 (1-2) ◽  
pp. 165-168 ◽  
Author(s):  
T.S. Rathore ◽  
R.P. Singh ◽  
N.S. Deora ◽  
N.S. Shekhawat
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation

Clonal propagation of someFreesia cultivars through tissue culture

1976 ◽  
Vol 18 (4) ◽  
pp. 304-306 ◽  
Author(s):  
Eva Petrů ◽  
Eva Jirsáková ◽  
Z. Landa
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation

The Clonal Propagation of Oil Palm by Tissue Culture

1986 ◽  
Vol 35 (5) ◽  
pp. 343-348
Author(s):  
Tadasu FUJITA
Keyword(s):  
Tissue Culture ◽  
Oil Palm ◽  
Clonal Propagation
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