Active Transport of Adenosine into Primary Cultures of Brain Cells and its Methodological Consequences

1991 ◽  
pp. 399-402
Author(s):  
Leif Hertz
Keyword(s):  
Active Transport ◽  
Primary Cultures ◽  
Brain Cells

Primary cultures of neuronal and non-neuronal rat brain cells secrete similar proportions of amyloid β peptides ending at Aβ40 and Aβ42

Neuroreport ◽  
1999 ◽  
Vol 10 (14) ◽  
pp. 2965-2969 ◽  
Author(s):  
Hiroaki Fukumoto ◽  
Taisuke Tomita ◽  
Hirokazu Matsunaga ◽  
Yoshihiro Ishibashi ◽  
Takaomi C. Saido ◽  
...  
Keyword(s):  
Rat Brain ◽  
Primary Cultures ◽  
Amyloid Β ◽  
Brain Cells

scholarly journals 184: Toxic effects of antibiotics routinely used in cell culture on primary cultures of dissociated mouse brain cells

1976 ◽  
Vol 10 (10) ◽  
pp. 900-900
Author(s):  
F Amonn ◽  
U Baumann ◽  
U Wiesmann ◽  
K Hofmann ◽  
N Herschkowitz
Keyword(s):  
Cell Culture ◽  
Mouse Brain ◽  
Primary Cultures ◽  
Toxic Effects ◽  
Brain Cells

Roles of IL-1 and TNF-alpha in endotoxin-induced activation of nitric oxide synthase in cultured rat brain cells

1996 ◽  
Vol 270 (2) ◽  
pp. R326-R332 ◽  
Author(s):  
L. I. Romero ◽  
J. B. Tatro ◽  
J. A. Field ◽  
S. Reichlin
Keyword(s):  
Nitric Oxide ◽  
Interleukin 1 ◽  
Primary Cultures ◽  
Neonatal Rat ◽  
Tnf Alpha ◽  
Brain Cells ◽  
Nitrite Accumulation ◽  
No Production ◽  
Necrosis Factor Alpha ◽  
Oxide Synthase

In astrocytes and microglia, bacterial lipopolysaccharide (LPS) stimulates production and release of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), and nitric oxide (NO). Although IL-1 beta and TNF-alpha are themselves capable of inducing NO synthase (NOS) in glia, the specific factors mediating LPS induction of NOS in brain have not been identified. To determine whether LPS induction of NOS in brain cells is mediated by IL-1 or TNF-alpha, acting alone or in concert, the effects of IL-1-receptor antagonist (IL-1Ra) and of TNF-soluble receptor (TNFsRp55), presented individually and in combination, on LPS-induced NOS activity were tested. In glial-enriched mixed primary cultures of neonatal rat telencephalic cells, LPS (0.1-100 ng/ml), IL-1 beta (0.01-10 nM), and TNF-alpha (0.1-100 nM) each concentration dependently stimulated accumulation of nitrite, an indicator of NO production. Induction of nitrite accumulation by LPS and by IL-1 was blocked by N omega-nitro-L-arginine methyl ester and N omega-monomethyl-L-arginine, indicating that it was mediated by NOS. TNF-alpha alone induced NO production weakly as compared with IL-1, but combined submaximal concentrations of IL-1 beta (1 nM) and TNF-alpha (10 nM) induced NOS synergistically. Furthermore, TNFsRp55 and IL-1Ra each produced a dose-dependent partial inhibition of the NO response to LPS, and the effect of TNFsRp55 was equal to or greater than that of IL-1Ra. TNFsRp55 and IL-1Ra in combination were not significantly more effective than TNF-sRp55 alone. The results indicate that LPS induction of NOS activity in brain cells is mediated in part by both IL-1 beta and TNF-alpha.

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