Replicative and Chronological Life-Span Assays

2014 ◽  
pp. 223-227 ◽  
Author(s):  
Spike D. L. Postnikoff ◽  
Troy A. A. Harkness
Keyword(s):  
Life Span ◽  
Chronological Life Span

SOD2 Functions Downstream of Sch9 to Extend Longevity in Yeast

Genetics ◽  
2003 ◽  
Vol 163 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Paola Fabrizio ◽  
Lee-Loung Liou ◽  
Vanessa N Moy ◽  
Alberto Diaspro ◽  
Joan Selverstone Valentine ◽  
...  
Keyword(s):  
Life Span ◽  
Stress Resistance ◽  
Reversible Inactivation ◽  
Life Span Extension ◽  
Resistance Proteins ◽  
Mitochondrial Superoxide ◽  
Mitochondrial Aconitase ◽  
Chronological Life Span ◽  
Survival Extension ◽  
Mitochondrial Superoxide Dismutase

Abstract Signal transduction pathways inactivated during periods of starvation are implicated in the regulation of longevity in organisms ranging from yeast to mammals, but the mechanisms responsible for life-span extension are poorly understood. Chronological life-span extension in S. cerevisiae cyr1 and sch9 mutants is mediated by the stress-resistance proteins Msn2/Msn4 and Rim15. Here we show that mitochondrial superoxide dismutase (Sod2) is required for survival extension in yeast. Deletion of SOD2 abolishes life-span extension in sch9Δ mutants and decreases survival in cyr1:mTn mutants. The overexpression of Sods—mitochondrial Sod2 and cytosolic CuZnSod (Sod1)—delays the age-dependent reversible inactivation of mitochondrial aconitase, a superoxide-sensitive enzyme, and extends survival by 30%. Deletion of the RAS2 gene, which functions upstream of CYR1, also doubles the mean life span by a mechanism that requires Msn2/4 and Sod2. These findings link mutations that extend chronological life span in S. cerevisiae to superoxide dismutases and suggest that the induction of other stress-resistance genes regulated by Msn2/4 and Rim15 is required for maximum longevity extension.

Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast

2017 ◽  
Vol 292 (2) ◽  
pp. 475-481 ◽  
Author(s):  
Takafumi Shimasaki ◽  
Hokuto Ohtsuka ◽  
Chikako Naito ◽  
Kenko Azuma ◽  
Takeshi Tenno ◽  
...  
Keyword(s):  
Life Span ◽  
Fission Yeast ◽  
Binding Protein ◽  
Zinc Binding ◽  
Chronological Life Span

Magnolol protects Saccharomyces cerevisiae antioxidant-deficient mutants from oxidative stress and extends yeast chronological life span

2019 ◽  
Vol 366 (8) ◽  
Author(s):  
Subasri Subramaniyan ◽  
Phaniendra Alugoju ◽  
Sudharshan SJ ◽  
Bhavana Veerabhadrappa ◽  
Madhu Dyavaiah
Keyword(s):  
Oxidative Stress ◽  
Saccharomyces Cerevisiae ◽  
Life Span ◽  
Chronological Life Span

scholarly journals Mitochondrial Respiratory Thresholds Regulate Yeast Chronological Life Span and its Extension by Caloric Restriction

2012 ◽  
Vol 16 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Alejandro Ocampo ◽  
Jingjing Liu ◽  
Elizabeth A. Schroeder ◽  
Gerald S. Shadel ◽  
Antoni Barrientos
Keyword(s):  
Life Span ◽  
Caloric Restriction ◽  
Chronological Life Span ◽  
Mitochondrial Respiratory

scholarly journals Autophagy is required for extension of yeast chronological life span by rapamycin

Autophagy ◽  
2009 ◽  
Vol 5 (6) ◽  
pp. 847-849 ◽  
Author(s):  
Ashley L. Alvers ◽  
Michael S. Wood ◽  
Doreen Hu ◽  
Amelia C. Kaywell ◽  
William A. Dunn, Jr. ◽  
...  
Keyword(s):  
Life Span ◽  
Chronological Life Span

scholarly journals The chronological life span of Saccharomyces cerevisiae

Aging Cell ◽  
2003 ◽  
Vol 2 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Paola Fabrizio ◽  
Valter D. Longo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Life Span ◽  
Chronological Life Span
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