Generation and Applications of MADM-Based Mouse Genetic Mosaic System

Abstract PR09: Fine temporal and spatial dissection of medulloblastoma progression with MADM, a mouse genetic mosaic model

10.1158/1538-7445.fbcr13-pr09 ◽

2013 ◽

Author(s):

Brit Ventura ◽

Maojin Yao ◽

Ying Jiang ◽

Kelsey Wahl ◽

Fausto Rodriguez ◽

...

Keyword(s):

Genetic Mosaic ◽

Mosaic Model ◽

Temporal And Spatial ◽

Mouse Genetic

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MADM-ML, a Mouse Genetic Mosaic System with Increased Clonal Efficiency

PLoS ONE ◽

10.1371/journal.pone.0077672 ◽

2013 ◽

Vol 8 (10) ◽

pp. e77672 ◽

Cited By ~ 8

Author(s):

Astra Henner ◽

P. Britten Ventura ◽

Ying Jiang ◽

Hui Zong

Keyword(s):

Genetic Mosaic ◽

Mouse Genetic

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Mouse Genetic Background Influences the Dental Phenotype

Cells Tissues Organs ◽

10.1159/000360157 ◽

2013 ◽

Vol 198 (6) ◽

pp. 448-456 ◽

Cited By ~ 7

Author(s):

Yong Li ◽

William S. Konicki ◽

J. Timothy Wright ◽

Cynthia Suggs ◽

Hui Xue ◽

...

Keyword(s):

Genetic Background ◽

Mouse Genetic

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The Genome Architecture of the Collaborative Cross Mouse Genetic Reference Population

Genetics ◽

10.1534/genetics.111.132639 ◽

2012 ◽

Vol 190 (2) ◽

pp. 389-401 ◽

Cited By ~ 313

Author(s):

Keyword(s):

Reference Population ◽

Collaborative Cross ◽

Genome Architecture ◽

Genetic Reference Population ◽

Mouse Genetic

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Generation and early differentiation of glial cells in the first optic ganglion of Drosophila melanogaster

Development ◽

10.1242/dev.115.4.903 ◽

1992 ◽

Vol 115 (4) ◽

pp. 903-911 ◽

Cited By ~ 6

Author(s):

M.L. Winberg ◽

S.E. Perez ◽

H. Steller

Keyword(s):

Drosophila Melanogaster ◽

Glial Cells ◽

Enhancer Trap ◽

P Element ◽

Cell Divisions ◽

Genetic Mosaic ◽

First Optic Ganglion ◽

Optic Ganglion ◽

Glial Lineage ◽

Enhancer Trap Lines

We have examined the generation and development of glial cells in the first optic ganglion, the lamina, of Drosophila melanogaster. Previous work has shown that the growth of retinal axons into the developing optic lobes induces the terminal cell divisions that generate the lamina monopolar neurons. We investigated whether photoreceptor ingrowth also influences the development of lamina glial cells, using P element enhancer trap lines, genetic mosaics and birthdating analysis. Enhancer trap lines that mark the differentiating lamina glial cells were found to require retinal innervation for expression. In mutants with only a few photoreceptors, only the few glial cells near ingrowing axons expressed the marker. Genetic mosaic analysis indicates that the lamina neurons and glial cells are readily separable, suggesting that these are derived from distinct lineages. Additionally, BrdU pulse-chase experiments showed that the cell divisions that produce lamina glia, unlike those producing lamina neurons, are not spatially or temporally correlated with the retinal axon ingrowth. Finally, in mutants lacking photoreceptors, cell divisions in the glial lineage appeared normal. We conclude that the lamina glial cells derive from a lineage that is distinct from that of the L-neurons, that glia are generated independently of photoreceptor input, and that completion of the terminal glial differentiation program depends, directly or indirectly, on an inductive signal from photoreceptor axons.

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FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain

Development ◽

10.1242/dev.129.16.3825 ◽

2002 ◽

Vol 129 (16) ◽

pp. 3825-3837 ◽

Cited By ~ 11

Author(s):

Lisa Maves ◽

William Jackman ◽

Charles B. Kimmel

Keyword(s):

Neuronal Differentiation ◽

Crucial Role ◽

Time Lapse ◽

Signaling Molecules ◽

Neural Plate ◽

Fgf Signaling ◽

Genetic Mosaic ◽

Signaling Center ◽

Signaling Activity ◽

Rhombomere 4

The segmentation of the vertebrate hindbrain into rhombomeres is highly conserved, but how early hindbrain patterning is established is not well understood. We show that rhombomere 4 (r4) functions as an early-differentiating signaling center in the zebrafish hindbrain. Time-lapse analyses of zebrafish hindbrain development show that r4 forms first and hindbrain neuronal differentiation occurs first in r4. Two signaling molecules, FGF3 and FGF8, which are both expressed early in r4, are together required for the development of rhombomeres adjacent to r4, particularly r5 and r6. Transplantation of r4 cells can induce expression of r5/r6 markers, as can misexpression of either FGF3 or FGF8. Genetic mosaic analyses also support a role for FGF signaling acting from r4. Taken together, our findings demonstrate a crucial role for FGF-mediated inter-rhombomere signaling in promoting early hindbrain patterning and underscore the significance of organizing centers in patterning the vertebrate neural plate.

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