Proteases of Human Rhinovirus: Role in Infection

2014 ◽  
pp. 129-141 ◽  
Author(s):  
Lora M. Jensen ◽  
Erin J. Walker ◽  
David A. Jans ◽  
Reena Ghildyal
Keyword(s):  
Human Rhinovirus

Successful in silico discovery of natural inhibitors for human rhinovirus coat protein

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
JM Rollinger ◽  
TM Steindl ◽  
K Anrain ◽  
EP Ellmerer ◽  
M Schmidtke ◽  
...  
Keyword(s):  
Coat Protein ◽  
In Silico ◽  
Human Rhinovirus ◽  
Natural Inhibitors

scholarly journals Dual inhibition of SARS-CoV-2 and human rhinovirus with protease inhibitors in clinical development

2021 ◽  
Vol 187 ◽  
pp. 105020
Author(s):  
Cheng Liu ◽  
Sandro Boland ◽  
Michael D. Scholle ◽  
Dorothee Bardiot ◽  
Arnaud Marchand ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Clinical Development ◽  
Human Rhinovirus ◽  
Dual Inhibition

High-Throughput Affinity Selection Mass Spectrometry Using SAMDI-MS to Identify Small-Molecule Binders of the Human Rhinovirus 3C Protease

2021 ◽  
pp. 247255522110232
Author(s):  
Michael D. Scholle ◽  
Doug McLaughlin ◽  
Zachary A. Gurard-Levin
Keyword(s):  
Mass Spectrometry ◽  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Human Rhinovirus ◽  
Binding Potential ◽  
Affinity Selection ◽  
Response Curves ◽  
Desorption Ionization ◽  
Self Assembled

Affinity selection mass spectrometry (ASMS) has emerged as a powerful high-throughput screening tool used in drug discovery to identify novel ligands against therapeutic targets. This report describes the first high-throughput screen using a novel self-assembled monolayer desorption ionization (SAMDI)–ASMS methodology to reveal ligands for the human rhinovirus 3C (HRV3C) protease. The approach combines self-assembled monolayers of alkanethiolates on gold with matrix-assisted laser desorption ionization time-of-flight (MALDI TOF) mass spectrometry (MS), a technique termed SAMDI-ASMS. The primary screen of more than 100,000 compounds in pools of 8 compounds per well was completed in less than 8 h, and informs on the binding potential and selectivity of each compound. Initial hits were confirmed in follow-up SAMDI-ASMS experiments in single-concentration and dose–response curves. The ligands identified by SAMDI-ASMS were further validated using differential scanning fluorimetry (DSF) and in functional protease assays against HRV3C and the related SARS-CoV-2 3CLpro enzyme. SAMDI-ASMS offers key benefits for drug discovery over traditional ASMS approaches, including the high-throughput workflow and readout, minimizing compound misbehavior by using smaller compound pools, and up to a 50-fold reduction in reagent consumption. The flexibility of this novel technology opens avenues for high-throughput ASMS assays of any target, thereby accelerating drug discovery for diverse diseases.

scholarly journals Interference Between Respiratory Syncytial Virus and Human Rhinovirus Infection in Infancy

2017 ◽  
Vol 215 (7) ◽  
pp. 1102-1106 ◽  
Author(s):  
Niek B. Achten ◽  
Pingsheng Wu ◽  
Louis Bont ◽  
Maarten O. Blanken ◽  
Tebeb Gebretsadik ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Human Rhinovirus ◽  
Rhinovirus Infection ◽  
Syncytial Virus
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