Ligation-Independent Cloning (LIC) Assembly of TALEN Genes

2014 ◽  
pp. 161-169 ◽  
Author(s):  
Jonathan L. Schmid-Burgk ◽  
Tobias Schmidt ◽  
Veit Hornung
Keyword(s):  
Ligation Independent Cloning

Expression Vectors for Enzyme Restriction- and Ligation-Independent Cloning for Producing Recombinant His-Fusion Proteins

2008 ◽  
Vol 23 (3) ◽  
pp. 680-686 ◽  
Author(s):  
Blanca de las Rivas ◽  
José Antonio Curiel ◽  
José Miguel Mancheño ◽  
Rosario Muñoz
Keyword(s):  
Fusion Proteins ◽  
Expression Vectors ◽  
Ligation Independent Cloning

scholarly journals One-Step Sequence- and Ligation-Independent Cloning as a Rapid and Versatile Cloning Method for Functional Genomics Studies

2012 ◽  
Vol 78 (15) ◽  
pp. 5440-5443 ◽  
Author(s):  
Jae-Yeon Jeong ◽  
Hyung-Soon Yim ◽  
Ji-Young Ryu ◽  
Hyun Sook Lee ◽  
Jung-Hyun Lee ◽  
...  
Keyword(s):  
Functional Genomics ◽  
Dna Polymerase ◽  
Room Temperature ◽  
Cost Effective ◽  
Time Saving ◽  
Ligation Independent Cloning ◽  
Effective Time ◽  
Cloning Method ◽  
Directional Cloning ◽  
One Step

ABSTRACTWe developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.

scholarly journals Rapid construction of mycobacterial mutagenesis vectors using ligation-independent cloning

2010 ◽  
Vol 83 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Ricardo Balhana ◽  
Neil G. Stoker ◽  
Mahmudul Hasan Sikder ◽  
Francois-Xavier Chauviac ◽  
Sharon L. Kendall
Keyword(s):  
Ligation Independent Cloning ◽  
Rapid Construction
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