Split-Luciferase Complementation Assay to Detect Channel–Protein Interactions in Live Cells

2015 ◽  
pp. 497-514 ◽  
Author(s):  
Alexander S. Shavkunov ◽  
Syed R. Ali ◽  
Neli I. Panova-Elektronova ◽  
Fernanda Laezza
Keyword(s):  
Protein Interactions ◽  
Live Cells ◽  
Complementation Assay ◽  
Channel Protein ◽  
Split Luciferase Complementation ◽  
Split Luciferase

scholarly journals Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format

Rice ◽  
2014 ◽  
Vol 7 (1) ◽  
Author(s):  
Yukichi Fujikawa ◽  
Takahiro Nakanishi ◽  
Hiroko Kawakami ◽  
Kanako Yamasaki ◽  
Masa H Sato ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Large Scale ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
Scale Format ◽  
Split Luciferase

LUCIFERASES FOR THE STUDY OF PROTEIN–PROTEIN INTERACTIONS IN LIVE CELLS AND ANIMALS

Nano LIFE ◽  
2010 ◽  
Vol 01 (01n02) ◽  
pp. 79-87 ◽  
Author(s):  
A. K. M. KAFI ◽  
MITSURU HATTORI ◽  
TAKEAKI OZAWA
Keyword(s):  
Transcriptional Regulation ◽  
Protein Interactions ◽  
Protein Modification ◽  
Specific Protein ◽  
Live Cells ◽  
Protein Protein Interactions ◽  
Recent Developments ◽  
Split Luciferase Complementation ◽  
Spatio Temporal ◽  
Split Luciferase

Many imaging technologies based on luminescent proteins have proven useful for detecting protein–protein interactions, tracking cells in mice, and monitoring transcriptional regulation of specific genes. Especially, novel bioluminescent proteins have advanced the study of induced protein interactions and protein modification in live cells and animals. This review focuses on recent developments of bioluminescent probes for quantitative evaluation of specific protein–protein interactions and their spatio-temporal imaging by means of split luciferase complementation techniques. From the comparison between fluorescent and bioluminescent proteins, advantages and drawbacks of the bioluminescence techniques are described.

scholarly journals Application of a split luciferase complementation assay for the detection of viral protein–protein interactions

2011 ◽  
Vol 176 (1-2) ◽  
pp. 108-111 ◽  
Author(s):  
Qiji Deng ◽  
Dan Wang ◽  
Xiaoxiao Xiang ◽  
Xiaofei Gao ◽  
Philip R. Hardwidge ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Viral Protein ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
Split Luciferase

A split luciferase complementation assay for studying in vivo protein–protein interactions in filamentous ascomycetes

2012 ◽  
Vol 58 (3) ◽  
pp. 179-189 ◽  
Author(s):  
Hee-Kyoung Kim ◽  
Eun Ji Cho ◽  
Seong mi Jo ◽  
Bo Reum Sung ◽  
Seunghoon Lee ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
Split Luciferase

scholarly journals A new split‐luciferase complementation assay identifies pentachlorophenol as an inhibitor of apoptosome formation

FEBS Open Bio ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1194-1203 ◽  
Author(s):  
Amin Tashakor ◽  
Mahshid H‐Dehkordi ◽  
Enda O'Connell ◽  
Sergi Gomez Ganau ◽  
Rafael Gozalbes ◽  
...  
Keyword(s):  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
Split Luciferase

Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae

2017 ◽  
Vol 114 (6) ◽  
pp. 1354-1361 ◽  
Author(s):  
Yosuke Fukutani ◽  
Jun Ishii ◽  
Akihiko Kondo ◽  
Takeaki Ozawa ◽  
Hiroaki Matsunami ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
G Protein ◽  
G Protein Coupled Receptor ◽  
Receptor Ligand ◽  
Complementation Assay ◽  
Split Luciferase Complementation ◽  
G Protein Coupled ◽  
Split Luciferase

Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system

The Analyst ◽  
2018 ◽  
Vol 143 (16) ◽  
pp. 3793-3797 ◽  
Author(s):  
Shogo Tsuji ◽  
Kouki Shinoda ◽  
Shiroh Futaki ◽  
Miki Imanishi
Keyword(s):  
Genomic Dna ◽  
Live Cells ◽  
System P ◽  
Split Luciferase Complementation ◽  
Split Luciferase

The newly developed TALE-split luciferase complementation system enabled sequence-specific 5mC detection of genomic DNA in live cells.

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