Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time

2015 ◽  
pp. 139-148 ◽  
Author(s):  
Xiaohua He ◽  
Stephanie A. Patfield
Keyword(s):  
Real Time ◽  
Sensitive Detection ◽  
Pcr Assay

Abstract 411: Sensitive detection of BRAF V600E mutation by bridged nucleic acid (BNA)-mediate real-time PCR assay

2016 ◽  
Author(s):  
Xiaoyun Liu ◽  
Leticia Loredo ◽  
Houquan Dai ◽  
Aaron Castro ◽  
Yuewei Zhao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Braf V600e Mutation ◽  
Braf V600e ◽  
Sensitive Detection ◽  
Pcr Assay

scholarly journals Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription-Real-Time Quantitative PCR Assay

2008 ◽  
Vol 46 (8) ◽  
pp. 2547-2554 ◽  
Author(s):  
I. Gutierrez-Aguirre ◽  
A. Steyer ◽  
J. Boben ◽  
K. Gruden ◽  
M. Poljsak-Prijatelj ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Sensitive Detection ◽  
Pcr Assay ◽  
Real Time Quantitative Pcr ◽  
Quantitative Pcr Assay

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae)

2020 ◽  
Author(s):  
Carlos Aguirre ◽  
Evelyn Sánchez ◽  
Natalia Olivares ◽  
Patricio Hinrichsen
Keyword(s):  
Real Time ◽  
Citrus Fruit ◽  
Sensitive Detection ◽  
Morphological Identification ◽  
Specific Detection ◽  
Pcr Assay ◽  
Polymerase Chain ◽  
Cost Efficient ◽  
Species Specific ◽  
Template Dna

Abstract Rapid and cost-efficient identification of Naupactus species is becoming a key process for the exportation of citrus fruit from Chile and other countries, considering the quarantine regulations for some species of the cosmopolitan genus Naupactus. This study deals with the development of a fast and sensitive detection protocol for Naupactus cervinus (Coleoptera: Curculionidae) (Boheman) and Naupactus xanthographus (Coleoptera: Curculionidae) (Germar) based on multiplex TaqMan Real-time polymerase chain reaction. Both N. cervinus and N. xanthographus primer and probe sets achieved species-specific detection in a linear range from 1 pg/μl to 1 × 10-6 pg/μl, allowing detection of as few as 160 copies of template DNA. Non-target amplifications were not detected and a panel composed of 480 test samples had 100% coincidence with the respective morphological identification.

scholarly journals Optimization of Real-Time PCR Assay for Rapid and Sensitive Detection of Eubacterial 16S Ribosomal DNA in Platelet Concentrates

2003 ◽  
Vol 41 (10) ◽  
pp. 4796-4798 ◽  
Author(s):  
T. Mohammadi ◽  
H. W. Reesink ◽  
C. M. J. E. Vandenbroucke-Grauls ◽  
P. H. M. Savelkoul
Keyword(s):  
Real Time ◽  
Ribosomal Dna ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Platelet Concentrates ◽  
Pcr Assay ◽  
16S Ribosomal Dna

Sensitive detection of porcine DNA in processed animal proteins using a TaqMan real-time PCR assay

2012 ◽  
Vol 29 (9) ◽  
pp. 1402-1412 ◽  
Author(s):  
N. Pegels ◽  
I. González ◽  
S. Fernández ◽  
T. García ◽  
R. Martín
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Pcr Assay

Ultra-sensitive detection of two garlic potyviruses using a real-time fluorescent (Taqman®) RT-PCR assay

2004 ◽  
Vol 118 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Pablo Lunello ◽  
Carmen Mansilla ◽  
Vilma Conci ◽  
Fernando Ponz
Keyword(s):  
Real Time ◽  
Sensitive Detection ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Development and validation of a real-time reverse transcriptase PCR assay for sensitive detection of SFTSV

2017 ◽  
Vol 89 (7) ◽  
pp. 1131-1138
Author(s):  
Peibin Zeng ◽  
Zhendong Yang ◽  
Sonia Bakkour ◽  
Bingjun Wang ◽  
Shuli Qing ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Sensitive Detection ◽  
Pcr Assay ◽  
Reverse Transcriptase Pcr ◽  
Development And Validation

scholarly journals Novel One-Step Single-Tube Nested Quantitative Real-Time PCR Assay for Highly Sensitive Detection of SARS-CoV-2

2020 ◽  
Vol 92 (13) ◽  
pp. 9399-9404 ◽  
Author(s):  
Ji Wang ◽  
Kun Cai ◽  
Ruiqing Zhang ◽  
Xiaozhou He ◽  
Xinxin Shen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Highly Sensitive ◽  
One Step ◽  
Highly Sensitive Detection
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