Isolation of Non-parenchymal Cells from the Mouse Liver

2015 ◽  
pp. 3-17 ◽  
Author(s):  
Isaac Mohar ◽  
Katherine J. Brempelis ◽  
Sara A. Murray ◽  
Mohammad R. Ebrahimkhani ◽  
I. Nicholas Crispe
Keyword(s):  
Mouse Liver ◽  
Parenchymal Cells

Differential abilities of mouse liver parenchymal and nonparenchymal cells in HDL and LDL (native and oxidized) association and cholesterol efflux

2006 ◽  
Vol 84 (2) ◽  
pp. 250-256 ◽  
Author(s):  
Jany Lapointe ◽  
To Quyen Truong ◽  
Louise Falstrault ◽  
Louise Brissette
Keyword(s):  
Mouse Liver ◽  
Cholesterol Efflux ◽  
Oxidized Ldl ◽  
Density Lipoprotein ◽  
Selective Uptake ◽  
Liver Parenchymal ◽  
Nonparenchymal Cells ◽  
Uptake Activity ◽  
Iodine 125 ◽  
Parenchymal Cells

The aim of this study was to quantify the abilities of mouse liver parenchymal and nonparenchymal cells with respect to (i) cholesteryl ester (CE) selective uptake from low-density lipoproteins (LDL), oxidized LDL (OxLDL), and high-density lipoprotein (HDL); and (ii) their free cholesterol efflux to HDL. The preparations of cells were incubated with lipoproteins labelled either in protein with iodine-125 or in CE with 3H-cholesterol oleate, and lipoprotein-protein and lipoprotein-CE associations were measured. The associations of LDL-protein and LDL-CE with nonparenchymal cells were 5- and 2-fold greater, respectively, than with parenchymal cells. However, in terms of CE-selective uptake (CE association minus protein association) both types of cell were equivalent. Similar results were obtained with OxLDL, but both types of cell showed higher abilities in OxLDL-CE than in LDL-CE selective uptake (on average by 3.4-fold). The association of HDL-protein with nonparenchymal cells was 3× that with parenchymal cells; however, nonparenchymal cells associated 45% less HDL-CE. Contrary to parenchymal cells, nonparenchymal cells did not show HDL-CE selective uptake activity. Thus parenchymal cells selectively take CE from the 3 types of lipoproteins, whereas nonparenchymal cells exert this function only on LDL and OxLDL. Efflux was 3.5-fold more important in nonparenchymal than in parenchymal cells.Key words: LDL, HDL, parenchymal, SR-BI, CD36, selective uptake, cholesterol.

scholarly journals Single-cell RNA transcriptome landscape of hepatocytes and non-parenchymal cells in healthy and NAFLD mouse liver

iScience ◽  
2021 ◽  
pp. 103233
Author(s):  
Qi Su ◽  
Sun Y. Kim ◽  
Funmi Adewale ◽  
Ye Zhou ◽  
Christina Aldler ◽  
...  
Keyword(s):  
Single Cell ◽  
Mouse Liver ◽  
Parenchymal Cells

scholarly journals A FINE STRUCTURE STUDY OF LIPID IN MOUSE LIVER REGENERATING AFTER PARTIAL HEPATECTOMY

1964 ◽  
Vol 21 (2) ◽  
pp. 233-244 ◽  
Author(s):  
Nancy L. Trotter
Keyword(s):  
Fine Structure ◽  
Partial Hepatectomy ◽  
Mouse Liver ◽  
Osmium Tetroxide ◽  
Structure Study ◽  
Regenerating Liver ◽  
Male Mice ◽  
Small Bodies ◽  
Cytoplasmic Bodies ◽  
Parenchymal Cells

The fine structure of liver 3½ to 72 hours after partial hepatectomy has been compared with that of liver from sham-operated animals; all animals were 60- to 90-day old male mice of the C3H strain. Numerous small bodies with diameters ranging from 300 to 1,000 A have been observed distributed randomly throughout the cytoplasm of the hepatic parenchymal cells at early intervals after partial hepatectomy. In material fixed in osmium tetroxide and embedded in methacrylate, they appear as uniformly electron-opaque bodies, but in permanganate-fixed liver, they display only a peripheral rim of electron-opaque material surrounding a clear core. Each of these cytoplasmic bodies appears to be located within a vesicle. A few of the opaque bodies are also present in sinusoids and in the spaces of Disse; these bodies are not located within vesicular structures. Fat droplets of various sizes are easily distinguished in regenerating liver; with the increase in number of these fat droplets at later postoperative intervals, there occurs a concomitant decrease in the number of cytoplasmic bodies. It is suggested that the cytoplasmic bodies contain some lipid component. Possible explanations of the origin, nature, and fate of the cytoplasmic bodies are discussed.

INTERLEUKIN 1 RECEPTOR ANTAGONIST LOCALIZATION EXCLUSIVELY IN NON-PARENCHYMAL CELLS OF THE MOUSE LIVER

Cytokine ◽  
1997 ◽  
Vol 9 (4) ◽  
pp. 249-253 ◽  
Author(s):  
Hanna Rokita ◽  
Magdalena Kosz-Vnenchak ◽  
Krystyna Stalińska ◽  
Jerzy Stachura
Keyword(s):  
Receptor Antagonist ◽  
Mouse Liver ◽  
Interleukin 1 ◽  
Interleukin 1 Receptor Antagonist ◽  
Parenchymal Cells

Electrogenic sodium pump in mouse liver parenchymal cells

1974 ◽  
Vol 187 (1088) ◽  
pp. 363-367 ◽  
Keyword(s):  
Mouse Liver ◽  
Sodium Pump ◽  
Microelectrode Recording ◽  
High Concentration ◽  
Gradual Reduction ◽  
Liver Parenchymal ◽  
Current Pulses ◽  
Electrogenic Sodium Pump ◽  
Set Up ◽  
Parenchymal Cells

Readmitting potassium to potassium-deprived mouse liver segments during intracellular microelectrode recording caused an increase in membrane potential and a gradual reduction in amplitude of electrotonic potentials set up by current pulses applied to another liver cell some distance away. Readmitting K in a high concentration (100 mM) caused a transient hyperpolarization followed by depolarization. The transient hyperpolarization was abolished by Strophanthin-G.

Sign in / Sign up

Export Citation Format

Share Document