Techniques for the Evaluation of the Genetic Expression, Intracellular Storage, and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (NPs)

2017 ◽  
pp. 163-176
Author(s):  
Adolfo J. de Bold ◽  
Mercedes L. de Bold
Keyword(s):  
Special Reference ◽  
Natriuretic Peptides ◽  
Genetic Expression ◽  
Polypeptide Hormones ◽  
Intracellular Storage

scholarly journals NATRIURETIC PEPTIDES AND THEIR ACTIONS, WITH SPECIAL REFERENCE TO THE DEVELOPMENT OF NEW DRUGS

1992 ◽  
Vol 7 (supplement) ◽  
pp. 591-593
Author(s):  
Hiroo IMURA ◽  
Kazuwa NAKAO
Keyword(s):  
Special Reference ◽  
Natriuretic Peptides ◽  
New Drugs

Detection of atrial natriuretic peptides (ANP) in rat atria by immunogold-silver staining (IGSS)

1994 ◽  
Vol 52 ◽  
pp. 304-305
Author(s):  
Robyn Rufner ◽  
Gerhard W. Hacker ◽  
Michele Forte ◽  
Nancyleigh E. Carson ◽  
Cristina Xenachis ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Silver Staining ◽  
Sodium Thiosulfate ◽  
Atrial Natriuretic Peptides ◽  
Paraffin Sections ◽  
Glass Slides ◽  
Metallic Salts ◽  
Wistar Kyoto ◽  
Right Atria

The use of immunogold-silver staining (IGSS) to enhance label penetration and Localization for immunocytochemistry or in situ hybridization utilizing a variety of metallic salts has been documented. In this morphological study, the effects of silver acetate, silver lactate and silver nitrate were evaluated for immunogold-labeling of a trial natriuretic peptides (ANP) in rat right atria.Eight Wistar Kyoto retired breeders were sedated with pentobarbital, perfused with either 4% paraformaldehyde (LM) or Karnovsky's fixative (EM), and right atria were dissected, processed, embedded in paraffin or epon, respectively and sectioned according to conventional methods. For light microscopy, an indirect IGSS method according to Hacker (3) was performed. Paraffin sections on glass slides were washed in ddH2O, immersed in Lugol's iodine, washed in ddH2O and treated with 2.5% aqueous sodium thiosulfate for 20 sec. After additional washes in ddH2O and TBS-0.1% fish gelatin, 10% normal goat serum (PBS with 1% BSA) was applied for 20 min before an overnight incubation at 4°C with a polyclonal α-ANP primary antibody (Peninsula Labs, 1:1000 in TBS/BSA).

A Comparative Study of the Colloidal Red Test in Liver Diseases with Special Reference to the Zinc Turbidity, Thymol Turbidity and Bromsulphalein Tests

1949 ◽  
Vol 12 (6) ◽  
pp. 934-948 ◽  
Author(s):  
Henry A. Strade ◽  
Louis B. Dotti ◽  
Stephanie J. Ilka
Keyword(s):  
Comparative Study ◽  
Special Reference ◽  
Liver Diseases ◽  
Thymol Turbidity

Molecular Biology of Atrial Natriuretic Peptides

1987 ◽  
Vol 16 (1) ◽  
pp. 43-61 ◽  
Author(s):  
Hisayuki Matsuo ◽  
Hiroshi Nakazato
Keyword(s):  
Molecular Biology ◽  
Natriuretic Peptides ◽  
Atrial Natriuretic Peptides ◽  
Atrial Natriuretic
Sign in / Sign up

Export Citation Format

Share Document