Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells

2017 ◽  
pp. 153-174 ◽  
Author(s):  
Xiuli An ◽  
Lixiang Chen
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
Erythroid Cells ◽  
Fluorescence Activated Cell Sorting ◽  
Fcm Analysis

scholarly journals High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow cytometry with fluorescence activated cell sorting

RSC Advances ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 4507-4513 ◽  
Author(s):  
Xu-Dong Zhu ◽  
Xiang Shi ◽  
Shu-Wen Wang ◽  
Ju Chu ◽  
Wei-Hong Zhu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Lactic Acid ◽  
High Throughput ◽  
Cell Sorting ◽  
High Throughput Screening ◽  
Bacillus Coagulans ◽  
Screening System ◽  
Fluorescence Activated Cell Sorting ◽  
Microfluidic Sorting

A high-throughput screening system based on droplet microfluidic sorting was developed and employed for screening of high lactic acid-producing Bacillus coagulans.

scholarly journals Application of flow cytometry and cell sorting to megakaryocytopoiesis

Blood ◽  
1979 ◽  
Vol 53 (4) ◽  
pp. 732-745 ◽  
Author(s):  
A Nakeff ◽  
F Valeriote ◽  
JW Gray ◽  
RJ Grabske
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
Significant Advance ◽  
Glass Slides ◽  
Acetylcholinesterase Staining ◽  
Fcm Analysis ◽  
Cell Fractions ◽  
Kinetics Of ◽  
Supravital Staining

Abstract We have employed flow cytometry (FCM) and cell sorting to quantitate and study megakaryocytes in mouse and rat femoral marrow following their 20- to 30-fold concentration by centrifugal elutriation (CE). This enrichment of megakaryocytes permitted the first determination of their DNA-related fluorescence by FCM analysis following DNA staining. Fluorescence distributions of CE-enriched cell fractions following supravital staining with Hoechst 33342 were similar to those following chromomycin A3 staining of ethanol-fixed cells. Microscopic examination of cells sorted onto glass slides on the basis of their DNA-related fluorescence following supravital staining together with specific acetylcholinesterase staining for megakaryocytes indicated that megakaryocytes generally increased in cell size with increasing DNA content. This technologic application represents a significant advance in the study of megakaryocytopoiesis, since the kinetics of either the normal or perturbed population can now be studied rapidly and quantitatively.

Flow Cytometry and Fluorescence-Activated Cell Sorting

2010 ◽  
pp. 19-1-19-39
Author(s):  
Chun-Hao Chen ◽  
Jessica Godin ◽  
Sung Cho ◽  
Frank Tsai ◽  
Wen Qiao ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Sorting ◽  
Fluorescence Activated Cell Sorting

A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting

Lab on a Chip ◽  
2004 ◽  
Vol 4 (6) ◽  
pp. 603 ◽  
Author(s):  
Bo Yao ◽  
Guo-an Luo ◽  
Xue Feng ◽  
Wei Wang ◽  
Ling-xin Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Device ◽  
Cell Sorting ◽  
Electric Force ◽  
Fluorescence Activated Cell Sorting
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