Analysis of Myelinating Schwann Cells in Human Skin Biopsies

2018 ◽  
pp. 359-369 ◽  
Author(s):  
Mario A. Saporta ◽  
Renata de Moraes Maciel
Keyword(s):  
Schwann Cells ◽  
Human Skin ◽  
Skin Biopsies

Changes in corneocyte element concentrations occur in skin inner stratum corneum

1990 ◽  
Vol 48 (2) ◽  
pp. 146-147
Author(s):  
R. R. Warner
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Element Concentration ◽  
Skin Barrier ◽  
Barrier Properties ◽  
Brick Wall ◽  
Inorganic Elements ◽  
Dry Skin ◽  
Skin Biopsies ◽  
Intercellular Lipid

Keratinocytes undergo maturation during their transit through the viable layers of skin, and then abruptly transform into flattened, anuclear corneocytes that constitute the cellular component of the skin barrier, the stratum corneum (SC). The SC is generally considered to be homogeneous in its structure and barrier properties, and is often shown schematically as a featureless brick wall, the “bricks” being the corneocytes, the “mortar” being intercellular lipid. Previously we showed the outer SC was not homogeneous in its composition, but contained steep gradients of the physiological inorganic elements Na, K and Cl, likely originating from sweat salts. Here we show the innermost corneocytes in human skin are also heterogeneous in composition, undergoing systematic changes in intracellular element concentration during transit into the interior of the SC.Human skin biopsies were taken from the lower leg of individuals with both “good” and “dry” skin and plunge-frozen in a stirred, cooled isopentane/propane mixture.

scholarly journals Laser capture microdissection for transcriptomic profiles in human skin biopsies

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Silvia Santoro ◽  
Ignazio Diego Lopez ◽  
Raffaella Lombardi ◽  
Andrea Zauli ◽  
Ana Maria Osiceanu ◽  
...  
Keyword(s):  
Human Skin ◽  
Laser Capture Microdissection ◽  
Skin Biopsies ◽  
Laser Capture

scholarly journals Three-dimensional Imaging of Nociceptive Intraepidermal Nerve Fibers in Human Skin Biopsies

10.3791/50331 ◽  
2013 ◽  
Author(s):  
Jacqueline R. Dauch ◽  
Chelsea N. Lindblad ◽  
John M. Hayes ◽  
Stephen I. Lentz ◽  
Hsinlin T. Cheng
Keyword(s):  
Human Skin ◽  
Three Dimensional ◽  
Nerve Fibers ◽  
Three Dimensional Imaging ◽  
Skin Biopsies

3D imaging of cleared human skin biopsies using light-sheet microscopy: A new way to visualize in-depth skin structure

2018 ◽  
Vol 24 (2) ◽  
pp. 294-303 ◽  
Author(s):  
S. Abadie ◽  
C. Jardet ◽  
J. Colombelli ◽  
B. Chaput ◽  
A. David ◽  
...  
Keyword(s):  
Human Skin ◽  
3D Imaging ◽  
Light Sheet ◽  
Skin Structure ◽  
Light Sheet Microscopy ◽  
Skin Biopsies

scholarly journals Schwann cells as underestimated, major players in human skin physiology and pathology

2019 ◽  
Vol 29 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Eric R. Bray ◽  
Jérémy Chéret ◽  
Gil Yosipovitch ◽  
Ralf Paus
Keyword(s):  
Schwann Cells ◽  
Human Skin ◽  
Skin Physiology

A semi-automated method to assess intraepidermal nerve fibre density in human skin biopsies

2015 ◽  
Vol 68 (5) ◽  
pp. 657-665 ◽  
Author(s):  
Shanon Seger ◽  
Manuel Stritt ◽  
Kathrin Doppler ◽  
Stephan Frank ◽  
Adrian Panaite ◽  
...  
Keyword(s):  
Nerve Fibre ◽  
Human Skin ◽  
Fibre Density ◽  
Automated Method ◽  
Skin Biopsies

Circadian clocks and tumor biology: What is to learn from human skin biopsies?

2013 ◽  
Vol 188 ◽  
pp. 67-74 ◽  
Author(s):  
Zsuzsanna Lengyel ◽  
Zita Battyáni ◽  
György Szekeres ◽  
Valér Csernus ◽  
András D. Nagy
Keyword(s):  
Human Skin ◽  
Tumor Biology ◽  
Circadian Clocks ◽  
Skin Biopsies

Proteolytic activation of prochemerin by kallikrein 7 breaks an ionic linkage and results in C-terminal rearrangement

2013 ◽  
Vol 452 (2) ◽  
pp. 271-280 ◽  
Author(s):  
Stephan Schultz ◽  
Anja Saalbach ◽  
John T. Heiker ◽  
Rene Meier ◽  
Tristan Zellmann ◽  
...  
Keyword(s):  
Human Skin ◽  
Underlying Mechanism ◽  
Structural Rearrangement ◽  
Proteolytic Activation ◽  
Psoriasis Therapy ◽  
Specific Protease ◽  
Skin Biopsies ◽  
Processing Protease ◽  
Excessive Accumulation

The excessive accumulation of adipose tissue in obesity is associated with multiple inflammatory dermatological diseases. Chemerin, a chemoattractant adipokine, dependent on proteolytical activation, is highly expressed in skin. Different proteases have been reported to activate prochemerin, but none is inherently expressed in human skin. In the present study, we identified a tissue-specific protease and investigated the underlying mechanism of activation at the molecular level. We characterized human KLK7 (kallikrein 7) as a prochemerin processing protease in vitro converting prochemerin into active chemerinF156. The activating truncation by the protease might trigger a structural rearrangement leading to an increased affinity of chemerin to CMKLR1 (chemokine-like receptor 1). Molecular modelling and experimental data suggest an underlying ionic interaction in prochemerin C-terminal domains. These findings provide a general molecular basis for the necessity of C-terminal processing of prochemerin. Moreover, immunohistochemistry was used to investigate prochemerin, KLK7 and the recently identified KLK7 inhibitor vaspin expression in human skin biopsies, and distinct co-localization in psoriatic biopsies was observed. On the basis of these results, it is hypothesized that KLK7 activity may contribute to the development of psoriatic lesions as a consequence of excessive chemerin activation and impaired protease activity regulation by vaspin. Therefore this interaction represents an interesting target for psoriasis therapy and treatment of other obesity-related diseases.

Expression of cytosolic retinoid-binding protein genes in human skin biopsies and cultured keratinocytes and fibroblasts

2006 ◽  
Vol 131 (2) ◽  
pp. 243-249 ◽  
Author(s):  
H. TÖRMÄ ◽  
W. LÖNTZ ◽  
WEI LIU ◽  
O. ROLLMAN ◽  
A. VAHLQUIST
Keyword(s):  
Human Skin ◽  
Binding Protein ◽  
Cultured Keratinocytes ◽  
Skin Biopsies
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