Measurement of the Caspase-1-Like Activity of Vacuolar Processing Enzyme in Plants

2018 ◽  
pp. 163-171 ◽  
Author(s):  
Noriyuki Hatsugai ◽  
Ikuko Hara-Nishimura
Keyword(s):  
Vacuolar Processing Enzyme ◽  
Caspase 1 ◽  
Processing Enzyme

scholarly journals Vacuolar Processing Enzymes Modulating Susceptibility Response to Fusarium oxysporum f. sp. cubense Tropical Race 4 Infections in Banana

2022 ◽  
Vol 12 ◽  
Author(s):  
Wan Muhamad Asrul Nizam Wan Abdullah ◽  
Noor Baity Saidi ◽  
Mohd Termizi Yusof ◽  
Chien-Yeong Wee ◽  
Hwei-San Loh ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Proteinase Inhibitors ◽  
Cysteine Proteinase ◽  
Resistant Cultivar ◽  
Susceptible Cultivar ◽  
Proteomic Profiling ◽  
Vacuolar Processing Enzyme ◽  
Caspase 1 ◽  
Processing Enzyme ◽  
Race 4

Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4) is a destructive necrotrophic fungal pathogen afflicting global banana production. Infection process involves the activation of programmed cell death (PCD). In this study, seven Musa acuminata vacuolar processing enzyme (MaVPE1–MaVPE7) genes associated with PCD were successfully identified. Phylogenetic analysis and tissue-specific expression categorized these MaVPEs into the seed and vegetative types. FocTR4 infection induced the majority of MaVPE expressions in the susceptible cultivar “Berangan” as compared to the resistant cultivar “Jari Buaya.” Consistently, upon FocTR4 infection, high caspase-1 activity was detected in the susceptible cultivar, while low level of caspase-1 activity was recorded in the resistant cultivar. Furthermore, inhibition of MaVPE activities via caspase-1 inhibitor in the susceptible cultivar reduced tonoplast rupture, decreased lesion formation, and enhanced stress tolerance against FocTR4 infection. Additionally, the Arabidopsis VPE-null mutant exhibited higher tolerance to FocTR4 infection, indicated by reduced sporulation rate, low levels of H2O2 content, and high levels of cell viability. Comparative proteomic profiling analysis revealed increase in the abundance of cysteine proteinase in the inoculated susceptible cultivar, as opposed to cysteine proteinase inhibitors in the resistant cultivar. In conclusion, the increase in vacuolar processing enzyme (VPE)-mediated PCD played a crucial role in modulating susceptibility response during compatible interaction, which facilitated FocTR4 colonization in the host.

Vacuolar Processing Enzyme Responsible for Maturation of Seed Proteins

1995 ◽  
Vol 145 (5-6) ◽  
pp. 632-640 ◽  
Author(s):  
Ikuko Hara-Nishimura ◽  
Tomoo Shimada ◽  
Nagako Hiraiwa ◽  
Mikio Nishimura
Keyword(s):  
Seed Proteins ◽  
Vacuolar Processing Enzyme ◽  
Processing Enzyme

scholarly journals The vacuolar processing enzyme OsVPE1 is required for efficient glutelin processing in rice

2009 ◽  
Vol 58 (4) ◽  
pp. 606-617 ◽  
Author(s):  
Yihua Wang ◽  
Susong Zhu ◽  
Shijia Liu ◽  
Ling Jiang ◽  
Liangming Chen ◽  
...  
Keyword(s):  
Vacuolar Processing Enzyme ◽  
Processing Enzyme

scholarly journals A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development

2005 ◽  
Vol 17 (3) ◽  
pp. 876-887 ◽  
Author(s):  
Satoru Nakaune ◽  
Kenji Yamada ◽  
Maki Kondo ◽  
Tomohiko Kato ◽  
Satoshi Tabata ◽  
...  
Keyword(s):  
Seed Development ◽  
Seed Coat ◽  
Early Stage ◽  
Vacuolar Processing Enzyme ◽  
Processing Enzyme

scholarly journals Vacuolar processing enzyme in plant programmed cell death

2015 ◽  
Vol 6 ◽  
Author(s):  
Noriyuki Hatsugai ◽  
Kenji Yamada ◽  
Shino Goto-Yamada ◽  
Ikuko Hara-Nishimura
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Vacuolar Processing Enzyme ◽  
Processing Enzyme

scholarly journals Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death

Autophagy ◽  
2020 ◽  
pp. 1-15
Author(s):  
Paula Teper-Bamnolker ◽  
Raz Danieli ◽  
Hadas Peled-Zehavi ◽  
Eduard Belausov ◽  
Mohamad Abu-Abied ◽  
...  
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Vacuolar Processing Enzyme ◽  
Processing Enzyme ◽  
Autophagy Pathway
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