Induction of Somatic Embryogenesis in Jatropha curcas

2018 ◽  
pp. 207-214
Author(s):  
Rosa M. Galaz-Ávalos ◽  
Heydi G. Martínez-Sánchez ◽  
Víctor M. Loyola-Vargas
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas

scholarly journals Effects of amino acids and spermidine on somatic embryogenesis of Jatropha curcas L.

2014 ◽  
Vol 35 (3se) ◽  
Author(s):  
Do Dang Giap ◽  
Nguyen Thi Kim Loan ◽  
Tran Trong Tuan ◽  
Le Thanh Tuan ◽  
Huynh Le Thien Tu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Jatropha Curcas L

scholarly journals Somatic Embryogenesis Media Optimization Study of Physic Nut (Jatropha curcas) as Biodiesel Feedstock

2014 ◽  
Vol 47 ◽  
pp. 21-28 ◽  
Author(s):  
Anggi Nindita ◽  
Bambang Sapta Purwoko ◽  
Darda Efendi ◽  
Iswari S. Dewi
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Media Optimization ◽  
Physic Nut ◽  
Optimization Study ◽  
Biodiesel Feedstock

scholarly journals Primary and secondary somatic embryogenesis in Jatropha curcas L. From leaf transverse thin cell layers

2018 ◽  
Vol 14 (4) ◽  
pp. 661-671
Author(s):  
Nguyen Thi Kim Loan ◽  
Do Dang Giap ◽  
Tran Trong Tuan ◽  
Nguyen Thi Thanh Hien ◽  
Nguyen Phuc Huy ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Somatic Embryos ◽  
Formation Rate ◽  
Ms Medium ◽  
Secondary Somatic Embryogenesis ◽  
Thin Cell Layers ◽  
Cell Layers ◽  
Secondary Embryos ◽  
Transverse Thin Cell Layers

An efficient method for plant regeneration in Jatropha curcas L. via primary and secondary somatic embryogenesis culture from ex vitro leaves of 6-month-old plants was presented in this study. Leaves were cut into transverse thin cell layers (tTCLs) and cultured on MS medium supplemented with kinetin (KIN) at 0.5, 1.0, 1.5, and 2.0 mg/l in combination with indole-3-butyric acid (IBA) at 0.1, 0.5, and 1.0 mg/l or 2,4-dichlorophenoxyacetic acid (2,4-D) at 1.0, 1.5 and 2.0 mg/l . The highest embryogenic callus formation rate (89.3%) was obtained on medium supplemented with 1.0 mg/l KIN and 1.5 mg/l 2,4-D. The calli were selected for the study of primary somatic embryogenesis on MS medium containing 2,4-D (0.01, 0.03, 0.05, and 0.07 mg/l) or KIN (0.5, 1.0, 1.5, and 2.0 mg/l). The highest primary somatic embryos formation rate (76.67%) was achieved on MS medium supplemented with 1.0 mg/l KIN. The primary embryos were cultured on medium supplemented with KIN (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) combined with 0.2 mg/l indole-3-butyric acid (IBA) or 0.05 mg/l 2,4-D. The combination of 1.5 mg/l KIN and 0.05 mg/l 2,4-D was suitable for secondary embryos formation. Embryos proliferated rapidly, and the highest number of secondary embryos (77.5 embryos) wasobtained from a single primary embryos inoculated. Results also showed that the addition of proline (0.75 g/l) or spermidine (0.15 mM) to the culture medium increased the number of secondary embryos considerably. The fully developed plantlets exhibiting healthy roots and shoots were obtained when somatic embryos were sub-cultured onto B5 medium containing 1.5 mg/l IBA.

Somatic Embryogenesis in Jatropha curcas

2016 ◽  
pp. 401-412 ◽  
Author(s):  
Rupesh Kumar Singh ◽  
Eliel Ruiz-May ◽  
Luz María García-Pérez ◽  
Francisco R. Quiroz-Figueroa
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas

Regeneration of Jatropha curcas through efficient somatic embryogenesis and suspension culture

GM Crops ◽  
2011 ◽  
Vol 2 (2) ◽  
pp. 110-117 ◽  
Author(s):  
Lin Cai ◽  
Lin Fu ◽  
Lianghui Ji
Keyword(s):  
Somatic Embryogenesis ◽  
Suspension Culture ◽  
Jatropha Curcas

Somatic embryogenesis in Jatropha curcas Linn., an important biofuel plant

2007 ◽  
Vol 1 (3) ◽  
pp. 135-140 ◽  
Author(s):  
Timir baran Jha ◽  
Priyanka Mukherjee ◽  
Mukul Manjari Datta
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas

scholarly journals Pengaruh Komposisi Media dan Sumber Eksplan Terhadap Induksi Kalus, Perkecambahan, dan Pertumbuhan Tunas Embrio Somatik Jarak Pagar

2016 ◽  
Vol 4 (2) ◽  
pp. 76
Author(s):  
Tantri Dyah Ayu Anggraeni ◽  
Emy Sulistyowati ◽  
Rully Dyah Purwati
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Jatropha Curcas ◽  
Stem Cuttings ◽  
Media Composition ◽  
Embryo Germination ◽  
Embryogenic Calli ◽  
Jatropha Curcas L ◽  
Somatic Embryo Germination ◽  
Somatic Embryogenic

<p>Jarak pagar (Jatropha curcas L.) merupakan tanaman penghasil minyak nabati sebagai bahan baku bio-diesel. Selama ini, kebutuhan bahan tanam diperoleh dari benih dan setek. Teknik mikropropagasi khususnya melalui embriogenesis somatik merupakan alternatif untuk penyediaan bahan tanam dalam jumlah besar dengan waktu relatif lebih singkat. Jenis eksplan, genotipe, dan kondisi fisiologis tanaman donor serta jenis dan kondisi fisik mediummerupakan faktor utama yang mempengaruhi keberhasilan embriogenesis somatik. Penelitian ini dilakukan untuk mengetahui eksplan dan komposisi media yang tepat untuk induksi kalus embriogenesis somatik, perkecambahan embrio somatik dan pertumbuhan tunas hasil embriogenesis somatik. Penelitian dilaksanakan di laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat mulai bulan April sampai dengan November 2011, meliputi tiga tahap, yaitu 1) menguji komposisi media untuk induksi kalus embriogenesis somatik antara lain M1=MS+0,5 mg/l BAP+0,5 mg 2,4 D; M2= MS+1 mg/l BAP +0,5 mg/l 2,4 D; M3= MS+0,5 mg/l BAP+0,2 mg/l TDZ, dan M4= MS+1 mg/l BAP+0,2 mg/l TDZ; 2) menguji komposisi media untuk induksi perkecambahan embrio somatik antara lain MK1= MS+0,5 mg/l BAP+0,1 mg/l NAA dan MK2= MS+0,5 mg/l BAP+0,4 mg/l IBA; dan 3) menguji komposisi media untuk pertumbuhan tunas embrio somatik antara lain MP1= MS+0,5 mg/l BAP+0,1 mg/l IBA dan MP2= MS+0,5 mg/l BAP+0,1 mg/l IAA. Bahan tanam yang digunakan adalah genotipe IP-3A dan IP-3M dengan sumber eksplan kotiledon dan daun. Hasil penelitian menunjukkan kombinasi MS+0,5 mg/l BAP+0,2 mg/l TDZ dengan sumber eksplan kotiledon paling sesuai untuk induksi kalus embriogenesis somatik. Genotipe IP-3M memiliki respon yang lebih baik disbanding IP-3A dan stabil dari tahap induksi kalus embriogenis somatik, induksi perkecambahan embrio somatik, dan pertumbuhan tunas embrio somatik.</p><p> </p><p>Jatropha (Jatropha curcas L.) is an oil producing plants as source of bio-diesel. Planting materials usually are obtained from seeds and stem-cuttings. Micro-propagation techniques especially through somatic embryo-genesis is an alternative to provide a large number of planting material in a relatively short time. Explant sources, genotype and physicological conditions of donor plants, also composition and physical condition of medium are the main factors affecting the successful of somatic embryogenesis. The study was conducted to determine the most suitable combination of explant and media composition for embryogenic calli induc-tion, somatic embryo germination, and shoots growth derived from somatic embryogenesis. The experiment was conducted in the Tissue Culture Laboratory, of Indonesian Sweetener and Fiber Crops Research Insti-tute from April to November 2011 covering three phases: 1) testing media composition to induce somatic embryogenic calli i.e. M1=MS+0.5 mg/l BAP+0.5 mg 2.4 D; M2 = MS+1 mg/l BAP+0.5 mg/l 2.4 D; M3 = MS+0.5 mg/l BAP+0.2 mg/l TDZ and M4 = MS+1 mg/l BAP+ 0.2 mg/l TDZ; 2) testing media composition to induce somatic embryo germination i.e. MK1 = MS+0.5 mg/l BAP+0.1 mg/l NAA and MK2 = MS+0.5 mg/l BAP+0.4 mg/l IBA; and 3) testing media composition to induce somatic embryo shoot growth i.e. MP1 = MS+0.5 mg/l BAP+0.1 mg/l IBA and MP2= MS+0.5 mg/l BAP+0.1 mg/l IAA. Plant material used are genotype IP-3A and IP-3M with cotyledone and leaf as explant sources. The results showed that combination of MS+0.5 mg/l BAP+0.2 mg/l TDZ and cotyledons as explants source is the most suitable for somatic embryogenic calli. IP-3M genotype showed a better response to IP-3A and stable from induction of somatic embryogenic calli, somatic embryo germination, and somatic embryo shoots growth.</p>

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