scholarly journals Somatic Embryogenesis from Leaf and Shoot tip Explants of Jatropha curcas L.

2014 ◽  
Vol 7 (11) ◽  
pp. 1842-1846 ◽  
Author(s):  
Srikanth Reddy Medipally
Keyword(s):  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Shoot Tip ◽  
Jatropha Curcas L ◽  
Shoot Tip Explants

scholarly journals Development of Efficient Micropropagation Protocols of Elite Accessions of Jatropha curcas L.

2014 ◽  
Vol 23 (2) ◽  
pp. 231-239
Author(s):  
B. Naresh ◽  
G. Dhanasri ◽  
M Srikanth Reddy ◽  
Prathibha Devi Cherku
Keyword(s):  
Jatropha Curcas ◽  
Adventitious Shoots ◽  
Cotyledonary Node ◽  
Botanical Garden ◽  
Axillary Node ◽  
Shoot Tip ◽  
Jatropha Curcas L ◽  
Shadow Area ◽  
Shoot Tip Explants ◽  
Cotyledonary Node Explants

Efficient micropropagation protocols were developed from explants of two accessions of Jatropha curcas L. viz. Rain Shadow Area Development (RSAD) department and Karimnagar (KM). Adventitious shoots were regenerated with higher frequency on medium-A from cotyledonary node explants, than those from axillary node and shoot-tip explants.  Shoots were also regenerated from calluses developed from cotyledonary node and shoot-tip explants on medium-B. Regenerated shoots were rooted on medium-G, transplanted to pots and later transferred to field. Somatic embryos developed on embryogenic calluses produced from cotyledonary node explants on medium-C were converted to plantlets on medium-F and transferred to pots. Out of the 199 plants established in the Botanical Garden, 118 plants were developed from cotyledonary node explants, which indicates that they are the most suitable explants for micropropagation of Jatropha. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17524 Plant Tissue Cult. & Biotech. 23(2): 231-239, 2013  (December)

scholarly journals Effects of amino acids and spermidine on somatic embryogenesis of Jatropha curcas L.

2014 ◽  
Vol 35 (3se) ◽  
Author(s):  
Do Dang Giap ◽  
Nguyen Thi Kim Loan ◽  
Tran Trong Tuan ◽  
Le Thanh Tuan ◽  
Huynh Le Thien Tu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Somatic Embryogenesis ◽  
Jatropha Curcas ◽  
Jatropha Curcas L

scholarly journals Pengaruh Komposisi Media dan Sumber Eksplan Terhadap Induksi Kalus, Perkecambahan, dan Pertumbuhan Tunas Embrio Somatik Jarak Pagar

2016 ◽  
Vol 4 (2) ◽  
pp. 76
Author(s):  
Tantri Dyah Ayu Anggraeni ◽  
Emy Sulistyowati ◽  
Rully Dyah Purwati
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Jatropha Curcas ◽  
Stem Cuttings ◽  
Media Composition ◽  
Embryo Germination ◽  
Embryogenic Calli ◽  
Jatropha Curcas L ◽  
Somatic Embryo Germination ◽  
Somatic Embryogenic

<p>Jarak pagar (Jatropha curcas L.) merupakan tanaman penghasil minyak nabati sebagai bahan baku bio-diesel. Selama ini, kebutuhan bahan tanam diperoleh dari benih dan setek. Teknik mikropropagasi khususnya melalui embriogenesis somatik merupakan alternatif untuk penyediaan bahan tanam dalam jumlah besar dengan waktu relatif lebih singkat. Jenis eksplan, genotipe, dan kondisi fisiologis tanaman donor serta jenis dan kondisi fisik mediummerupakan faktor utama yang mempengaruhi keberhasilan embriogenesis somatik. Penelitian ini dilakukan untuk mengetahui eksplan dan komposisi media yang tepat untuk induksi kalus embriogenesis somatik, perkecambahan embrio somatik dan pertumbuhan tunas hasil embriogenesis somatik. Penelitian dilaksanakan di laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat mulai bulan April sampai dengan November 2011, meliputi tiga tahap, yaitu 1) menguji komposisi media untuk induksi kalus embriogenesis somatik antara lain M1=MS+0,5 mg/l BAP+0,5 mg 2,4 D; M2= MS+1 mg/l BAP +0,5 mg/l 2,4 D; M3= MS+0,5 mg/l BAP+0,2 mg/l TDZ, dan M4= MS+1 mg/l BAP+0,2 mg/l TDZ; 2) menguji komposisi media untuk induksi perkecambahan embrio somatik antara lain MK1= MS+0,5 mg/l BAP+0,1 mg/l NAA dan MK2= MS+0,5 mg/l BAP+0,4 mg/l IBA; dan 3) menguji komposisi media untuk pertumbuhan tunas embrio somatik antara lain MP1= MS+0,5 mg/l BAP+0,1 mg/l IBA dan MP2= MS+0,5 mg/l BAP+0,1 mg/l IAA. Bahan tanam yang digunakan adalah genotipe IP-3A dan IP-3M dengan sumber eksplan kotiledon dan daun. Hasil penelitian menunjukkan kombinasi MS+0,5 mg/l BAP+0,2 mg/l TDZ dengan sumber eksplan kotiledon paling sesuai untuk induksi kalus embriogenesis somatik. Genotipe IP-3M memiliki respon yang lebih baik disbanding IP-3A dan stabil dari tahap induksi kalus embriogenis somatik, induksi perkecambahan embrio somatik, dan pertumbuhan tunas embrio somatik.</p><p> </p><p>Jatropha (Jatropha curcas L.) is an oil producing plants as source of bio-diesel. Planting materials usually are obtained from seeds and stem-cuttings. Micro-propagation techniques especially through somatic embryo-genesis is an alternative to provide a large number of planting material in a relatively short time. Explant sources, genotype and physicological conditions of donor plants, also composition and physical condition of medium are the main factors affecting the successful of somatic embryogenesis. The study was conducted to determine the most suitable combination of explant and media composition for embryogenic calli induc-tion, somatic embryo germination, and shoots growth derived from somatic embryogenesis. The experiment was conducted in the Tissue Culture Laboratory, of Indonesian Sweetener and Fiber Crops Research Insti-tute from April to November 2011 covering three phases: 1) testing media composition to induce somatic embryogenic calli i.e. M1=MS+0.5 mg/l BAP+0.5 mg 2.4 D; M2 = MS+1 mg/l BAP+0.5 mg/l 2.4 D; M3 = MS+0.5 mg/l BAP+0.2 mg/l TDZ and M4 = MS+1 mg/l BAP+ 0.2 mg/l TDZ; 2) testing media composition to induce somatic embryo germination i.e. MK1 = MS+0.5 mg/l BAP+0.1 mg/l NAA and MK2 = MS+0.5 mg/l BAP+0.4 mg/l IBA; and 3) testing media composition to induce somatic embryo shoot growth i.e. MP1 = MS+0.5 mg/l BAP+0.1 mg/l IBA and MP2= MS+0.5 mg/l BAP+0.1 mg/l IAA. Plant material used are genotype IP-3A and IP-3M with cotyledone and leaf as explant sources. The results showed that combination of MS+0.5 mg/l BAP+0.2 mg/l TDZ and cotyledons as explants source is the most suitable for somatic embryogenic calli. IP-3M genotype showed a better response to IP-3A and stable from induction of somatic embryogenic calli, somatic embryo germination, and somatic embryo shoots growth.</p>

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