Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry

2018 ◽  
pp. 255-264 ◽  
Author(s):  
Tânia G. Lucas ◽  
Cláudio M. Gomes ◽  
Bárbara J. Henriques
Keyword(s):  
Misfolded Proteins ◽  
Differential Scanning Fluorimetry ◽  
Thermal Shift

scholarly journals SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions

2015 ◽  
Author(s):  
Changye Sun ◽  
Yong Li ◽  
Edwin A Yates ◽  
David G Fernig
Keyword(s):  
Data Analysis ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Protein Ligand Interactions ◽  
Melting Curves ◽  
Differential Scanning Fluorimetry ◽  
Ligand Interactions ◽  
Protein Thermal Stability ◽  
User Friendly ◽  
Thermal Shift

Differential scanning fluorimetry (DSF) is used widely as a thermal shift assay to study protein stability and protein-ligand interactions. The benefit of DSF is that it is simple, cheap and can generate melting curves in 96-well plates providing good throughput. However, data analysis remains a challenge, and requires different methods to optimise and analyse the collected raw data. Here, the program SimpleDSFviewer is introduced to help view and analyse DSF data in an efficient way and with a user-friendly interface. The data analysis, optimisation and view methods provided by the program are described, using sample melting curves of fibroblast growth factors.

scholarly journals SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions

2015 ◽  
Author(s):  
Changye Sun ◽  
Yong Li ◽  
Edwin A Yates ◽  
David G Fernig
Keyword(s):  
Data Analysis ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Protein Ligand Interactions ◽  
Melting Curves ◽  
Differential Scanning Fluorimetry ◽  
Ligand Interactions ◽  
Protein Thermal Stability ◽  
User Friendly ◽  
Thermal Shift

Differential scanning fluorimetry (DSF) is used widely as a thermal shift assay to study protein stability and protein-ligand interactions. The benefit of DSF is that it is simple, cheap and can generate melting curves in 96-well plates providing good throughput. However, data analysis remains a challenge, and requires different methods to optimise and analyse the collected raw data. Here, the program SimpleDSFviewer is introduced to help view and analyse DSF data in an efficient way and with a user-friendly interface. The data analysis, optimisation and view methods provided by the program are described, using sample melting curves of fibroblast growth factors.

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