scholarly journals How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

10.3791/58666 ◽  
2019 ◽  
Author(s):  
Daniel Bruce ◽  
Emily Cardew ◽  
Stefanie Freitag-Pohl ◽  
Ehmke Pohl
Keyword(s):  
Protein Stability ◽  
Differential Scanning Fluorimetry ◽  
Thermal Shift

Protein Stability Using Thermal Shift Assay (TSA): pH Tolerance

2021 ◽  
pp. 35-41
Author(s):  
Srinivasan Swaminathan ◽  
A. L. Theerthan
Keyword(s):  
Protein Stability ◽  
Thermal Shift Assay ◽  
Ph Tolerance ◽  
Thermal Shift

Differential Scanning Fluorimetry Measurement of Protein Stability Changes upon Binding to Glycosaminoglycans: A Screening Test for Binding Specificity

2010 ◽  
Vol 82 (9) ◽  
pp. 3796-3802 ◽  
Author(s):  
Katarzyna A. Uniewicz ◽  
Alessandro Ori ◽  
Ruoyan Xu ◽  
Yassir Ahmed ◽  
Mark C. Wilkinson ◽  
...  
Keyword(s):  
Protein Stability ◽  
Screening Test ◽  
Binding Specificity ◽  
Differential Scanning Fluorimetry

Protein Stability Using Thermal Shift Assay (TSA): DMSO Tolerance

2021 ◽  
pp. 43-50
Author(s):  
V. Swarnakumari ◽  
A. L. Theerthan
Keyword(s):  
Protein Stability ◽  
Thermal Shift Assay ◽  
Thermal Shift

scholarly journals SimpleDSFviewer: a tool to analyse and view differential scanning fluorimetry data for characterising protein thermal stability and interactions

2015 ◽  
Author(s):  
Changye Sun ◽  
Yong Li ◽  
Edwin A Yates ◽  
David G Fernig
Keyword(s):  
Data Analysis ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Protein Ligand Interactions ◽  
Melting Curves ◽  
Differential Scanning Fluorimetry ◽  
Ligand Interactions ◽  
Protein Thermal Stability ◽  
User Friendly ◽  
Thermal Shift

Differential scanning fluorimetry (DSF) is used widely as a thermal shift assay to study protein stability and protein-ligand interactions. The benefit of DSF is that it is simple, cheap and can generate melting curves in 96-well plates providing good throughput. However, data analysis remains a challenge, and requires different methods to optimise and analyse the collected raw data. Here, the program SimpleDSFviewer is introduced to help view and analyse DSF data in an efficient way and with a user-friendly interface. The data analysis, optimisation and view methods provided by the program are described, using sample melting curves of fibroblast growth factors.

scholarly journals Membrane (and Soluble) Protein Stability and Binding Measurements in the Lipid Cubic Phase Using Label-Free Differential Scanning Fluorimetry

2018 ◽  
Vol 90 (20) ◽  
pp. 12152-12160 ◽  
Author(s):  
Coilín Boland ◽  
Samir Olatunji ◽  
Jonathan Bailey ◽  
Nicole Howe ◽  
Dietmar Weichert ◽  
...  
Keyword(s):  
Protein Stability ◽  
Soluble Protein ◽  
Cubic Phase ◽  
Label Free ◽  
Differential Scanning Fluorimetry

Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry

2011 ◽  
Vol 13 (30) ◽  
pp. 13614 ◽  
Author(s):  
João V. Rodrigues ◽  
Vesna Prosinecki ◽  
Isabel Marrucho ◽  
Luís Paulo N. Rebelo ◽  
Cláudio M. Gomes
Keyword(s):  
Ionic Liquid ◽  
Protein Stability ◽  
Differential Scanning Fluorimetry
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