Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes

2018 ◽  
pp. 403-430 ◽  
Author(s):  
Ellen J. Collarini ◽  
Philip A. Leighton ◽  
Marie-Cecile Van de Lavoir
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Transgenic Chickens

scholarly journals Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens

2020 ◽  
Vol 41 (3) ◽  
pp. 281-291
Author(s):  
Zi-Qin Jiang ◽  
◽  
Han-Yu Wu ◽  
Jing Tian ◽  
Ning Li ◽  
...  
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Lentiviral Vectors ◽  
Efficient Strategy ◽  
Transgenic Chickens

Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector

2010 ◽  
Vol 109 (4) ◽  
pp. 315-321 ◽  
Author(s):  
Makoto Motono ◽  
Yuki Yamada ◽  
Yuki Hattori ◽  
Ryo Nakagawa ◽  
Ken-ichi Nishijima ◽  
...  
Keyword(s):  
Germ Cells ◽  
Lentiviral Vector ◽  
Primordial Germ Cells ◽  
Transgenic Chickens

Primordial germ cells (PGCs) as a tool for creating transgenic chickens

2012 ◽  
Vol 15 (1) ◽  
pp. 181-188 ◽  
Author(s):  
L. Chojnacka-Puchta ◽  
K. Kasperczyk ◽  
G. Płucienniczak ◽  
D. Sawicka ◽  
M. Bednarczyk
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Heterologous Proteins ◽  
Transgenic Chicken ◽  
Migration Pathway ◽  
Pharmaceutical Proteins ◽  
Alternative Approach ◽  
Recent Developments ◽  
Transgenic Chickens

Primordial germ cells (PGCs) as a tool for creating transgenic chickens The transgenic chicken has great potential as a bioreactor for the production of valuable pharmaceutical proteins, notably in the oviduct/egg. Whereas conventional transgenic approaches have significant limitations in this species, an alternative approach employing primordial germ cells (PGCs), the progenitor cells to ova and spermatozoa, has now been successfully applied to the insertion of exogenous genes into birds. Recent developments in manipulating avian embryos make it possible to produce germline chimeras derived from transferred PGCs. In this review we describe the migration pathway of chicken PGCs during early development. We then summarize different methods for the isolation of PGCs and the diversity of techniques used to introduce genes into these cells. Finally, we describe an in vitro assay for testing tissue-specific vectors designed to express heterologous proteins in transgenic chickens.

Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

1992 ◽  
Vol 50 (1) ◽  
pp. 668-669
Author(s):  
Amreek Singh ◽  
Warren G. Foster ◽  
Anna Dykeman ◽  
David C. Villeneuve
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Surface Epithelium ◽  
Morphological Parameters ◽  
Comprehensive Investigation ◽  
Ovary Surface Epithelium ◽  
Rat Ovary ◽  
High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

Transfusion of Chick Primordial Germ Cells into Quail Embryos and their Settlement in Gonads

1998 ◽  
Vol 69 (10) ◽  
pp. 911-915 ◽  
Author(s):  
Tamao ONO ◽  
Ryohei YOKOI ◽  
Seishi MAEDA ◽  
Takao NISHIDA ◽  
Hirohiko AOYAMA
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells
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