Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development

2019 ◽  
pp. 3-21
Author(s):  
Frank Costantini
Keyword(s):  
Kidney Development ◽  
Mouse Embryos ◽  
Genetic Mosaic

scholarly journals Klf6 Is a Zinc Finger Protein Expressed in a Cell-Specific Manner during Kidney Development

2001 ◽  
Vol 12 (4) ◽  
pp. 726-735
Author(s):  
EVELYNE A. FISCHER ◽  
MARIE-CHRISTINE VERPONT ◽  
LEE ANN GARRETT-SINHA ◽  
PIERRE M. RONCO ◽  
JEROME A. ROSSERT
Keyword(s):  
Zinc Finger ◽  
Kidney Development ◽  
Zinc Finger Protein ◽  
Collecting Duct ◽  
Mouse Embryos ◽  
Wolffian Duct ◽  
Duct System ◽  
Finger Protein ◽  
Mesangial Area ◽  
Renal Collecting Duct

Abstract. Molecular mechanisms that are responsible for the development of the renal collecting duct system during embryogenesis are still poorly understood. A mouse cDNA encoding a zinc finger protein, called Klf6, which is a member of the Krüppel-like family of transcription factors, has been cloned. Northern blot analyses showed that Klf6 was already expressed in 11.5-d postconception mouse embryos and that its expression persisted after birth. They also disclosed that Klf6 had a restricted pattern of expression. In situ hybridization experiments using mouse embryos showed that during kidney development, Klf6 was expressed selectively in the Wolffian duct and in its derivatives. During mesonephros development, it was expressed in the Wolffian duct but not in the mesonephric mesenchyme. Thereafter, Klf6 was expressed in the ureteric bud and its branches and in the collecting ducts, whereas it was not expressed in tubular structures that derive from the metanephric mesenchyme. Glomeruli were not labeled during early stages of differentiation, and it is only at the capillary stage that a staining of the mesangial area was observed, which persisted after birth. This pattern of expression is strikingly similar to the one of GATA-3, which is another zinc finger protein. It suggests that Klf6 may play a role during kidney development and in particular during the development of the renal collecting duct system, possibly in association with GATA-3.

scholarly journals Kidney Regeneration in Later-Stage Mouse Embryos via Transplanted Renal Progenitor Cells

2019 ◽  
Vol 30 (12) ◽  
pp. 2293-2305 ◽  
Author(s):  
Shuichiro Yamanaka ◽  
Yatsumu Saito ◽  
Toshinari Fujimoto ◽  
Tsuyoshi Takamura ◽  
Susumu Tajiri ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cell Transplantation ◽  
Progenitor Cell ◽  
Kidney Development ◽  
Fluorescent Protein ◽  
Mouse Embryos ◽  
Kidney Regeneration ◽  
Renal Progenitor Cells ◽  
Renal Progenitor

BackgroundThe limited availability of donor kidneys for transplantation has spurred interest in investigating alternative strategies, such as regenerating organs from stem cells transplanted into animal embryos. However, there is no known method for transplanting cells into later-stage embryos, which may be the most suitable host stages for organogenesis, particularly into regions useful for kidney regeneration.MethodsWe demonstrated accurate transplantation of renal progenitor cells expressing green fluorescent protein to the fetal kidney development area by incising the opaque uterine muscle layer but not the transparent amniotic membrane. We allowed renal progenitor cell–transplanted fetuses to develop for 6 days postoperatively before removal for analysis. We also transplanted renal progenitor cells into conditional kidney-deficient mouse embryos. We determined growth and differentiation of transplanted cells in all cases.ResultsRenal progenitor cell transplantation into the retroperitoneal cavity of fetuses at E13–E14 produced transplant-derived, vascularized glomeruli with filtration function and did not affect fetal growth or survival. Cells transplanted to the nephrogenic zone produced a chimera in the cap mesenchyme of donor and host nephron progenitor cells. Renal progenitor cells transplanted to conditional kidney-deficient fetuses induced the formation of a new nephron in the fetus that is connected to the host ureteric bud.ConclusionsWe developed a cell transplantation method for midstage to late-stage fetuses. In vivo kidney regeneration from renal progenitor cells using the renal developmental environment of the fetus shows promise. Our findings suggest that fetal transplantation methods may contribute to organ regeneration and developmental research.

Fulminant metanephric apoptosis and abnormal kidney development in bcl-2-deficient mice

1995 ◽  
Vol 268 (1) ◽  
pp. F73-F81 ◽  
Author(s):  
C. M. Sorenson ◽  
S. A. Rogers ◽  
S. J. Korsmeyer ◽  
M. R. Hammerman
Keyword(s):  
Growth And Development ◽  
Kidney Development ◽  
Apoptotic Cell ◽  
Mouse Embryos ◽  
Kidney Morphology ◽  
Metanephric Kidney ◽  
Renal Size ◽  
Culture Growth ◽  
Renal Organogenesis

Apoptosis of the developing metanephric kidney plays an important role in renal organogenesis. The bcl-2 is an oncogene that inhibits apoptotic cell death in a variety of settings. The bcl-2 (-/-) mice complete embryonic development but, in contrast to bcl-2 (+/-) and bcl-2 (+/+) littermates, manifest growth retardation, hypopigmentation of hair, lymphoid apoptosis, abnormal kidney morphology, and renal failure postnatally. To provide insight into the mechanism for the latter abnormalities, we examined metanephric kidneys from bcl-2 (-/-), bcl-2 (+/-), and bcl-2 (+/+) mice, as well as embryonic day 12 (E12) mouse embryos, and compared growth and development of metanephroi in vitro. Kidneys from bcl-2 (+/-) mice developed normally. In contrast, development of kidneys from bcl-2 (-/-) mice was abnormal as reflected by a marked reduction of renal size in newborns compared with kidneys of bcl-2 (+/-) littermates. In addition, kidneys from bcl-2 (-/-) mice contained far fewer nephrons and had smaller nephrogenic zones. Although metanephroi obtained from E12 bcl-2 (+/-) and bcl-2 (-/-) mouse embryos were comparable in size, apoptosis of cells within metanephric blastemas of metanephroi from E12 bcl-2 (-/-) embryos was strikingly enhanced compared with that in blastemas of metanephroi from bcl-2 (+/-) embryos. During 3 days in culture, growth and development of metanephroi from bcl-2 (-/-) embryos were visibly reduced compared with those from bcl-2 (+/-) embryos.(ABSTRACT TRUNCATED AT 250 WORDS)

Human Adenovirus Uptake by Preirnplantation Mouse Embryos

1972 ◽  
Vol 30 ◽  
pp. 268-269
Author(s):  
D. G. Chase ◽  
W. Winters ◽  
L. Piko
Keyword(s):  
Hela Cells ◽  
Human Adenovirus ◽  
Mouse Embryos ◽  
Equilibrium Density ◽  
Cell Stage ◽  
Virus Attachment ◽  
Preimplantation Mouse Embryos ◽  
Embryo Culture Medium ◽  
Preimplantation Mouse ◽  
Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

Preplate neurons in embryonic mouse cortex: Ultrastructural observations using photoconversion of the fluorescent lipophilic dye dil

1992 ◽  
Vol 50 (1) ◽  
pp. 906-907
Author(s):  
Linda C. Hassinger ◽  
James E. Crandall
Keyword(s):  
Mouse Embryos ◽  
Embryonic Mouse ◽  
Cortical Afferents ◽  
Mouse Cortex ◽  
Carbocyanine Dye ◽  
Increased Sensitivity

We have begun to look directly at small numbers of afferent axons to early generated neurons that form the preplate in the developing mouse cortex. The carbocyanine dye Dil (1’1, dioctadecyl-3,3,3’3’-tetramethyl-indocarbocyanine) has proved especially useful for this goal. DiI labels axons and their terminals with greater sensitivity and without some of the disadvantages of axon filling with HRP. The increased sensitivity provided by labeling embryonic axons with DiI has given us new insights into the development of cortical afferents. For instance, we reported originally that afferents from the thalamus were present below the cortex as early as embryonic day 15 (E15) based on HRP injections into mouse embryos. By using DiI placements into the thalamus in aldehyde-fixed brains, we now know that thalamic fibers reach the cortex 24 hrs earlier.

Localization of Intracisternal a Particle Antigens in Neoplastic Cells and Preimplantation Mouse Embryos

1980 ◽  
Vol 38 ◽  
pp. 696-697
Author(s):  
Thomas T.F. Huang ◽  
Patricia G. Calarco
Keyword(s):  
Endoplasmic Reticulum ◽  
Normal Development ◽  
Mouse Embryos ◽  
Neoplastic Cells ◽  
Large Numbers ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Intracisternal A Particle ◽  
Normal Feature

The stage specific appearance of a retravirus, termed the Intracisternal A particle (IAP) is a normal feature of early preimplantation development. To date, all feral and laboratory strains of Mus musculus and even Asian species such as Mus cervicolor and Mus pahari express the particles during the 2-8 cell stages. IAP form by budding into the endoplasmic reticulum and appear singly or as groups of donut-shaped particles within the cisternae (fig. 1). IAP are also produced in large numbers in several neoplastic cells such as certain plasmacytomas and rhabdomyosarcomas. The role of IAP, either in normal development or in neoplastic behavior, is unknown.

Incorporation of substrate carbon into mouse embryos during culture in media containing sodium pyruvate

Reproduction ◽  
1970 ◽  
Vol 21 (2) ◽  
pp. 374-374
Author(s):  
R. Wales ◽  
D. Whittingham
Keyword(s):  
Mouse Embryos ◽  
Sodium Pyruvate

Effect of blastocyst artificial collapse prior to vitrification on pluripotency-specific genes expression in mouse embryos

2014 ◽  
Author(s):  
Mojtaba Dashtizad ◽  
Mehdi Shamsara ◽  
Morteza Daliri ◽  
Ghazaleh Zandi ◽  
Parisa Fathalizadeh ◽  
...  
Keyword(s):  
Mouse Embryos ◽  
Genes Expression

Diabetic environment and genetic predisposition as causes of congenital malformations in NOD mouse embryos

Diabetes ◽  
1991 ◽  
Vol 40 (10) ◽  
pp. 1245-1250 ◽  
Author(s):  
H. Otani ◽  
O. Tanaka ◽  
R. Tatewaki ◽  
H. Naora ◽  
T. Yoneyama
Keyword(s):  
Genetic Predisposition ◽  
Congenital Malformations ◽  
Mouse Embryos ◽  
Nod Mouse

PGE2 prevents anomalies induced by hyperglycemia or diabetic serum in mouse embryos

Diabetes ◽  
1992 ◽  
Vol 41 (12) ◽  
pp. 1644-1650 ◽  
Author(s):  
M. P. Goto ◽  
A. S. Goldman ◽  
M. R. Uhing
Keyword(s):  
Mouse Embryos
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