Heme•G-Quadruplex DNAzymes: Conditions for Maximizing Their Peroxidase Activity

2019 ◽  
pp. 357-368
Author(s):  
Nisreen Shumayrikh ◽  
Dipankar Sen
Keyword(s):  
Peroxidase Activity ◽  
G Quadruplex

Backbone modification promotes peroxidase activity of G-quadruplex-based DNAzyme

2012 ◽  
Vol 48 (67) ◽  
pp. 8347 ◽  
Author(s):  
Cong Li ◽  
Ling Zhu ◽  
Zhi Zhu ◽  
Hao Fu ◽  
Gareth Jenkins ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
G Quadruplex ◽  
Backbone Modification

Characterization of the G-quadruplex structure of a catalytic DNA with peroxidase activity

Biopolymers ◽  
2009 ◽  
Vol 91 (5) ◽  
pp. 331-339 ◽  
Author(s):  
De-Ming Kong ◽  
Li-Li Cai ◽  
Jun-Hong Guo ◽  
Jing Wu ◽  
Han-Xi Shen
Keyword(s):  
Peroxidase Activity ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Catalytic Dna

G-quadruplex-proximity protein labeling based on peroxidase activity

2020 ◽  
Vol 56 (78) ◽  
pp. 11641-11644
Author(s):  
Tatsuki Masuzawa ◽  
Shinichi Sato ◽  
Tatsuya Niwa ◽  
Hideki Taguchi ◽  
Hiroyuki Nakamura ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
Protein Labeling ◽  
G Quadruplex ◽  
Close Proximity

G-quadruplex-proximity protein labeling was performed using a hemin-parallel G-quadruplex (G4) complex. A tyrosine labeling reaction was accelerated in close proximity to the hemin with enhanced peroxidase activity by binding to parallel G4.

scholarly journals Increasing the activity of DNAzyme based on the telomeric sequence: 2’-OMe-RNA and LNA modifications

2019 ◽  
Vol 17 (1) ◽  
pp. 1157-1166
Author(s):  
J. Kosman ◽  
K. Żukowski ◽  
B. Juskowiak
Keyword(s):  
Dna Sequences ◽  
Peroxidase Activity ◽  
Telomeric Sequence ◽  
Modified Oligonucleotides ◽  
Biological Applications ◽  
Telomeric Dna ◽  
Nucleotide Analogues ◽  
Dna Oligonucleotides ◽  
G Quadruplex ◽  
Low Activity

Abstract2’-OMe-RNA analogues and LNA point modifications of DNA oligonucleotides were applied for the modulation of the G-quadruplex topology and enhancement of peroxidase activity of the resulting DNAzymes. The effect of the 2’-OMe-RNA analogue was studied for full length modified oligonucleotides with various sequences. In the case of LNA-point modification, we have chosen a telomeric DNA sequence and investigated various numbers of modifications. Our main goal was to prove that the application of these modifications can influence the activity of DNAzyme, especially those, which normally form poor DNAzymes. As an example, we have chosen the telomeric HT22 sequence which is known to form DNAzyme characterized by low activity. In all cases, the DNAzymes formed by a telomeric sequence with the application of the 2’-OMe-RNA analogue as well as LNA-point modification, showed significantly higher peroxidase activity. We were also able to shift the formation of hybrid or antiparallel topology to parallel topology. These results are important for the development of probes for biological applications as well as for the design of probes based on DNA sequences that normally form DNAzymes with low activity. This paper also provides information on how the application of nucleotide analogues can transform the topology of G-quadruplexes.

A G‐Quadruplex/Hemin Complex with Switchable Peroxidase Activity by DNA Hybridization

2012 ◽  
Vol 30 (7) ◽  
pp. 1575-1581 ◽  
Author(s):  
Congying Shao ◽  
Na Lu ◽  
Dengming Sun
Keyword(s):  
Peroxidase Activity ◽  
Dna Hybridization ◽  
G Quadruplex

General Peroxidase Activity of G-Quadruplex−Hemin Complexes and Its Application in Ligand Screening

Biochemistry ◽  
2009 ◽  
Vol 48 (33) ◽  
pp. 7817-7823 ◽  
Author(s):  
Xiaohong Cheng ◽  
Xiangjun Liu ◽  
Tao Bing ◽  
Zehui Cao ◽  
Dihua Shangguan
Keyword(s):  
Peroxidase Activity ◽  
Ligand Screening ◽  
G Quadruplex

Peroxidase Activity of G-Quadruplex Hemin-Binding DNA Aptamers Determined by Electrochemical Measurement

2013 ◽  
Vol 50 (28) ◽  
pp. 1-7 ◽  
Author(s):  
I. Kubo ◽  
T. Eguchi ◽  
Y. Hoshino ◽  
M. Liu ◽  
H. Abe ◽  
...  
Keyword(s):  
Peroxidase Activity ◽  
Electrochemical Measurement ◽  
Dna Aptamers ◽  
G Quadruplex
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